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. 1979 Apr;36(1):102–106.

Histochemical studies for 5'-nucleotidase and alpha-naphthyl (non-specific) esterase in lymphocytes from patients with primary immunoglobulin deficiencies.

N Matamoros, D A Horwitz, C Newton, G L Asherson, A D Webster
PMCID: PMC1537683  PMID: 466856

Abstract

Lymphocytes from patients with primary immunodeficiency were tested histochemically for ecto 5'-nucleotidase (5'N) and alpha-naphthyl (non-specific) esterase. More than half the patients with 'common variable' hypogammaglobulinaemia, all those with X-linked hypogammaglobulinaemia and some of those with selective IgA deficiency had a very low percentage of lymphocytes staining for 5'N as compared to controls. A lack of B cells probably explains the finding in X-linked hypogammaglobulinaemia, but does not fully explain the results in the other groups. Most patients with 'common variable' hypogammaglobulinaemia had a very low percentage of lymphocytes with granular staining for alpha-naphthyl (non-specific) esterase in contrast to normal numbers in those with X-linked hypogammaglobulinaemia and most of those with selective IgA deficiency. Granules containing non-specific esterase are characteristically found in 'mature' T lymphocytes. The enzyme abnormalities in the T and B cells of 'common variable' hypogammaglobulinaemic patients could be explained by 'immature' cell types.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown G., Greaves M. F. Cell surface markers for human T and B lymphocytes. Eur J Immunol. 1974 Apr;4(4):302–310. doi: 10.1002/eji.1830040414. [DOI] [PubMed] [Google Scholar]
  2. Gordon J., Howlett A. R., Smith J. L. Free light chain synthesis by neoplastic cells in chronic lymphocytic leukaemia and non-Hodgkin's lymphoma. Immunology. 1978 Mar;34(3):397–404. [PMC free article] [PubMed] [Google Scholar]
  3. Grossi C. E., Webb S. R., Zicca A., Lydyard P. M., Moretta L., Mingari M. C., Cooper M. D. Morphological and histochemical analyses of two human T-cell subpopulations bearing receptors for IgM or IgG. J Exp Med. 1978 May 1;147(5):1405–1417. doi: 10.1084/jem.147.5.1405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hayward A. R., Lawton A. R. Induction of plasma cell differentiation of human fetal lymphocytes: evidence for functional immaturity of T and B cells. J Immunol. 1977 Oct;119(4):1213–1217. [PubMed] [Google Scholar]
  5. Horwitz D. A., Allison A. C., Ward P., Kight N. Identification of human mononuclear leucocyte populations by esterase staining. Clin Exp Immunol. 1977 Nov;30(2):289–298. [PMC free article] [PubMed] [Google Scholar]
  6. Horwitz D. A., Webster A. D., Newton C. B and L lymphocytes in primary hypogammaglobulinaemia. Lancet. 1977 Oct 15;2(8042):823–824. doi: 10.1016/s0140-6736(77)90758-9. [DOI] [PubMed] [Google Scholar]
  7. Johnson S. M., North M. E., Asherson G. L., Allsop J., Watts R. W., Webster A. D. Lymphocyte purine 5'-nucleotidase edficiency in primary hypogammaglobulinaemia. Lancet. 1977 Jan 22;1(8004):168–170. doi: 10.1016/s0140-6736(77)91765-2. [DOI] [PubMed] [Google Scholar]
  8. Kulenkampff J., Janossy G., Greaves M. F. Acid esterase in human lymphoid cells and leukaemic blasts: a marker for T lymphocytes. Br J Haematol. 1977 Jun;36(2):231–240. doi: 10.1111/j.1365-2141.1977.tb00644.x. [DOI] [PubMed] [Google Scholar]
  9. Mueller J., Keller H. U., Brun del Re G., Buerki H., Hess M. W. Nonspecific esterase activity in T cells. Adv Exp Med Biol. 1976;66:117–122. doi: 10.1007/978-1-4613-4355-4_17. [DOI] [PubMed] [Google Scholar]
  10. Nell P. A., Ammann A. J., Hong R., Stiehm E. R. Familial selective IgA deficiency. Pediatrics. 1972 Jan;49(1):71–79. [PubMed] [Google Scholar]
  11. Pichler W. J., Lum L., Broder S. Fc-receptors on human T lymphocytes. I. Transition of Tgamma to Tmu cells. J Immunol. 1978 Oct;121(4):1540–1548. [PubMed] [Google Scholar]
  12. Rowe M., de Gast C. G., Platts-Mills T. A., Asherson G. L., Webster A. D., Johnson S. M. 5'-nucleotidase of B and T lymphocytes isolated from human peripheral blood. Clin Exp Immunol. 1979 Apr;36(1):97–101. [PMC free article] [PubMed] [Google Scholar]
  13. Silber R., Conklyn M., Grusky G., Zucker-Franklin D. Human lymphocytes: 5'-nucleotidase-positive and -negative subpopulations. J Clin Invest. 1975 Nov;56(5):1324–1327. doi: 10.1172/JCI108209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Waldmann T. A., Durm M., Broder S., Blackman M., Blaese R. M., Strober W. Role of suppressor T cells in pathogenesis of common variable hypogammaglobulinaemia. Lancet. 1974 Sep 14;2(7881):609–613. doi: 10.1016/s0140-6736(74)91940-0. [DOI] [PubMed] [Google Scholar]
  15. Webster A. D., Asherson G. L. Identification and function of T cells in the peripheral blood of patients with hypogammaglobulinaemia. Clin Exp Immunol. 1974 Dec;18(4):499–504. [PMC free article] [PubMed] [Google Scholar]
  16. Webster A. D., North M., Allsop J., Asherson G. L., Watts R. W. Purine metabolism in lymphocytes from patients with primary hypogammaglobulinaemia. Clin Exp Immunol. 1978 Mar;31(3):456–463. [PMC free article] [PubMed] [Google Scholar]
  17. de la Concha E. G., Oldham G., Webster A. D., Asherson G. L., Platts-Mills T. A. Quantitative measurements of T- and B-cell function in "variable" primary hypogammaglobulinaemia: evidence for a consistent B-cell defect. Clin Exp Immunol. 1977 Feb;27(2):208–215. [PMC free article] [PubMed] [Google Scholar]

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