Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1979 Apr;36(1):78–84.

Altered regulation of mitogen responsiveness by suppressor cells in multiple sclerosis.

R L Gonzalez, P C Dau, L E Spitler
PMCID: PMC1537686  PMID: 313860

Abstract

Suppression of the lymphocyte response to concanavalin A (Con A), phytohaemagglutinin (PHA) and protein A from Staphylococcus aureus (SpA) by Con A-induced suppressor cells was measured in twenty-four patients with recently active-recovering multiple sclerosis (MS), twelve with inactive MS and twenty-three healthy controls. Patients with recently active disease displayed significantly greater suppression of the response to Con A. Suppression of the responses to PHA and SpA did not differ among the groups. Lymphocyte stimulation in cultures not showing suppression was similar in all three types of subjects. These results suggest a disturbance of lymphocyte regulation in patients with recently active-recovering MS and illustrate the potential usefulness of measuring the suppression of responsiveness to several mitogens.

Full text

PDF
78

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Antel J. P., Weinrich M., Arnason B. G. Circulating suppressor cells in man as a function of age. Clin Immunol Immunopathol. 1978 Jan;9(1):134–141. doi: 10.1016/0090-1229(78)90130-7. [DOI] [PubMed] [Google Scholar]
  2. Arnason B. G., Antel J. Suppressor cell function in multiple sclerosis. Ann Immunol (Paris) 1978 Feb-Mar;129(2-3):159–170. [PubMed] [Google Scholar]
  3. Boldt S., Skinner A. M., Kornfeld S. Studies of two subpopulations of human lymphocytes differing in responsiveness to concanavalin A. J Clin Invest. 1972 Dec;51(12):3225–3234. doi: 10.1172/JCI107149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brody J. A., Sever J. L., Edgar A., McNew J. Measles antibody titers of multiple sclerosis patients and their siblings. Neurology. 1972 May;22(5):492–499. doi: 10.1212/wnl.22.5.492. [DOI] [PubMed] [Google Scholar]
  5. Caspary E. A. Humoral factors involved in immune processes in multiple sclerosis and allergic encephalomyelitis. Br Med Bull. 1977 Jan;33(1):50–53. doi: 10.1093/oxfordjournals.bmb.a071396. [DOI] [PubMed] [Google Scholar]
  6. De Gast G. C., The T. H., Ponds E., Kallenberg C. Suppression of DNA synthesis by Con A-activated human lymphocytes: Stimulation by con A bound to non-T cells unless removed after activation. Clin Exp Immunol. 1977 Dec;30(3):457–464. [PMC free article] [PubMed] [Google Scholar]
  7. Diamond B., Knight S. C., Lance E. M. Some observations on the in vitro reactivity of lymphoid subpopulations. Cell Immunol. 1974 Mar 30;11(1-3):239–246. doi: 10.1016/0008-8749(74)90024-0. [DOI] [PubMed] [Google Scholar]
  8. Esiri M. M. Immunoglobulin-containing cells in multiple-sclerosis plaques. Lancet. 1977 Sep 3;2(8036):478–478. doi: 10.1016/s0140-6736(77)91603-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Forsgren A., Svedjelund A., Wigzell H. Lymphocyte stimulation by protein A of Staphylococcus aureus. Eur J Immunol. 1976 Mar;6(3):207–213. doi: 10.1002/eji.1830060312. [DOI] [PubMed] [Google Scholar]
  10. Ganrot K., Laurell C. B. Measurement of IgG and albumin content of cerebrospinal fluid, and its interpretation. Clin Chem. 1974 May;20(5):571–573. [PubMed] [Google Scholar]
  11. Hallgren H. M., Yunis E. J. Suppressor lymphocytes in young and aged humans. J Immunol. 1977 Jun;118(6):2004–2008. [PubMed] [Google Scholar]
  12. Hodgson H. J., Wands J. R., Isselbacher K. J. Alteration in suppressor cell activity in chronic active hepatitis. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1549–1553. doi: 10.1073/pnas.75.3.1549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Horowitz S., Borcherding W., Moorthy A. V., Chesney R., Schulte-Wisserman H., Hong R. Induction of suppressor T cells in systemic lupus erythematosus by thymosin and cultured thymic epithelium. Science. 1977 Sep 2;197(4307):999–1001. doi: 10.1126/science.302032. [DOI] [PubMed] [Google Scholar]
  14. Hubert C., Delespesse G., Govaerts A. Concanavalin A-activated suppressor cells in normal human peripheral blood lymphocytes. Clin Exp Immunol. 1976 Oct;26(1):95–98. [PMC free article] [PubMed] [Google Scholar]
  15. Häyry P., Roberts P. J., Ranki A., Weber T. Selective responses of mouse T lymphocytes to different T mitogens and in mixed lymphocyte culture induced cytolysis reaction. Cell Immunol. 1976 Jul;25(1):121–128. doi: 10.1016/0008-8749(76)90102-7. [DOI] [PubMed] [Google Scholar]
  16. Lumsden C. E. The immunogenesis of the multiple sclerosis plaque. Brain Res. 1971 May 21;28(3):365–390. doi: 10.1016/0006-8993(71)90052-7. [DOI] [PubMed] [Google Scholar]
  17. Moretta L., Webb S. R., Grossi C. E., Lydyard P. M., Cooper M. D. Functional analysis of two human T-cell subpopulations: help and suppression of B-cell responses by T cells bearing receptors for IgM or IgG. J Exp Med. 1977 Jul 1;146(1):184–200. doi: 10.1084/jem.146.1.184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Möller G., Landwall P. The polyclonal B-cell-activating property of protein A is not due to its interaction with the FC part of immunoglobulin receptors. Scand J Immunol. 1977;6(4):357–366. doi: 10.1111/j.1365-3083.1977.tb00405.x. [DOI] [PubMed] [Google Scholar]
  19. Ozer H., Jr, Waksman B. H. The response of rabbit lymphocytes to mitogens and alloantigens: evidence for T cell heterogeneity. J Immunol. 1974 Dec;113(6):1780–1792. [PubMed] [Google Scholar]
  20. Rawson A. J., Daniele R. P. The separation of lymphocytes responsive only to concanavalin A from those also responsive to phytohemagglutinin. J Reticuloendothel Soc. 1976 Dec;20(6):421–427. [PubMed] [Google Scholar]
  21. Rawson A. J., Huang T. C. The response to phytohemagglutinin or to concanavalin A as a probe for subpopulations of human peripheral blood lymphocytes. Cell Immunol. 1975 May;17(1):310–314. doi: 10.1016/s0008-8749(75)80032-3. [DOI] [PubMed] [Google Scholar]
  22. Sakane T., Green I. Human suppressor T cells induced by concanavalin A: suppressor T cells belong to distinctive T cell subclasses. J Immunol. 1977 Sep;119(3):1169–1178. [PubMed] [Google Scholar]
  23. Sakane T., Green I. Protein A from Staphylococcus aureus-a mitogen for human T lymphocytes and B lymphocytes but not L lymphocytes. J Immunol. 1978 Jan;120(1):302–311. [PubMed] [Google Scholar]
  24. Santoli D., Moretta L., Lisak R., Gilden D., Koprowski H. Imbalances in T cell subpopulations in multiple sclerosis patients. J Immunol. 1978 Apr;120(4):1369–1371. [PubMed] [Google Scholar]
  25. Shou L., Schwartz S. A., Good R. A. Suppressor cell activity after concanavalin A treatment of lymphocytes from normal donors. J Exp Med. 1976 May 1;143(5):1100–1110. doi: 10.1084/jem.143.5.1100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Stobo J. D. Phytohemagglutin and concanavalin A: probes for murine 'T' cell activivation and differentiation. Transplant Rev. 1972;11:60–86. doi: 10.1111/j.1600-065x.1972.tb00046.x. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES