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. 1979 Apr;36(1):97–101.

5'-nucleotidase of B and T lymphocytes isolated from human peripheral blood.

M Rowe, C G de Gast, T A Platts-Mills, G L Asherson, A D Webster, S M Johnson
PMCID: PMC1537698  PMID: 313862

Abstract

The ecto-5'-nucleotidase activities of highly purified T and B lymphocytes from human peripheral blood have been investigated using biochemical and histochemical techniques. The enzyme activity of the purified B cells was about 3.5 times that of the T cells. Using a histochemical assay, 21--55% of the B cells stained positively for 5'-nucleotidase, but only 2--22% of the T cells were positive. These results are discussed in relation to the low 5'-nucleotidase activities found on peripheral blood lymphocytes from patients with chronic lymphatic leukaemia and some patients with primary hypogammaglobulinaemia.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avruch J., Wallach D. F. Preparation and properties of plasma membrane and endoplasmic reticulum fragments from isolated rat fat cells. Biochim Biophys Acta. 1971 Apr 13;233(2):334–347. doi: 10.1016/0005-2736(71)90331-2. [DOI] [PubMed] [Google Scholar]
  2. Belfield A., Goldberg D. M. Inhibition of the nucleotidase effect of alkaline phosphatase by beta-glycerophosphate. Nature. 1968 Jul 6;219(5149):73–75. doi: 10.1038/219073a0. [DOI] [PubMed] [Google Scholar]
  3. Berman J. D., Johnson W. D., Jr Monocyte function in human neonates. Infect Immun. 1978 Mar;19(3):898–902. doi: 10.1128/iai.19.3.898-902.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cooper M. D., Lawton A. R. Circulating B-cells in patients with immunodeficiency. Am J Pathol. 1972 Dec;69(3):513–528. [PMC free article] [PubMed] [Google Scholar]
  5. DePierre J. W., Karnovsky M. L. Ecto-enzymes of the guinea pig polymorphonuclear leukocyte. I. Evidence for an ecto-adenosine monophosphatase, adenosine triphosphatase, and -p-nitrophenyl phosphates. J Biol Chem. 1974 Nov 25;249(22):7111–7120. [PubMed] [Google Scholar]
  6. DePierre J. W., Karnovsky M. L. Ecto-enzymes of the guinea pig polymorphonuclear leukocyte. II. Properties and suitability as markers for the plasma membrane. J Biol Chem. 1974 Nov 25;249(22):7121–7129. [PubMed] [Google Scholar]
  7. Edwards N. L., Magilavy D. B., Cassidy J. T., Fox I. H. Lymphocyte ecto-5'-nucleotidase deficiency in agammaglobulinemia. Science. 1978 Aug 18;201(4356):628–630. doi: 10.1126/science.27864. [DOI] [PubMed] [Google Scholar]
  8. Gmelig-Meyling F., Ballieux R. E. Simplified procedure for the separation of human T and non-T cells. Vox Sang. 1977 Jul;33(1):5–8. doi: 10.1111/j.1423-0410.1977.tb02229.x. [DOI] [PubMed] [Google Scholar]
  9. Johnson S. M., North M. E., Asherson G. L., Allsop J., Watts R. W., Webster A. D. Lymphocyte purine 5'-nucleotidase edficiency in primary hypogammaglobulinaemia. Lancet. 1977 Jan 22;1(8004):168–170. doi: 10.1016/s0140-6736(77)91765-2. [DOI] [PubMed] [Google Scholar]
  10. Lopes J., Zucker-Franklin D., Silber R. Heterogeneity of 5'-nucleotidase activity in lymphocytes in chronic lymphocytic leukemia. J Clin Invest. 1973 May;52(5):1297–1300. doi: 10.1172/JCI107298. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Müller-Hermelink H. K. Characterization of the B-cell and T-cell regions of human lymphatic tissue through enzyme histochemical demonstration of ATPase and 5'-nucleotidase activities. Virchows Arch B Cell Pathol. 1974;16(4):371–378. doi: 10.1007/BF02894089. [DOI] [PubMed] [Google Scholar]
  12. Parish C. R., Hayward J. A. The lymphocyte surface. I. Relation between Fc receptors, C'3 receptors and surface immunoglobulin. Proc R Soc Lond B Biol Sci. 1974 Aug 27;187(1086):47–63. doi: 10.1098/rspb.1974.0060. [DOI] [PubMed] [Google Scholar]
  13. Quagliata F., Faig D., Conklyn M., Silber R. Studies on the lymphocyte 5'-nucleotidase in chronic lymphocytic leukemia, infectious mononucleosis, normal subpopulations, and phytohemagglutinin-stimulated cells. Cancer Res. 1974 Dec;34(12):3197–3202. [PubMed] [Google Scholar]
  14. Silber R., Conklyn M., Grusky G., Zucker-Franklin D. Human lymphocytes: 5'-nucleotidase-positive and -negative subpopulations. J Clin Invest. 1975 Nov;56(5):1324–1327. doi: 10.1172/JCI108209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Uusitalo R. J., Karnovsky M. J. 5'-Nucleotidase in different populations of mouse lymphocytes. J Histochem Cytochem. 1977 Feb;25(2):97–103. doi: 10.1177/25.2.300085. [DOI] [PubMed] [Google Scholar]
  16. Webster A. D., North M., Allsop J., Asherson G. L., Watts R. W. Purine metabolism in lymphocytes from patients with primary hypogammaglobulinaemia. Clin Exp Immunol. 1978 Mar;31(3):456–463. [PMC free article] [PubMed] [Google Scholar]

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