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. 1979 Apr;36(1):8–15.

Circulating antibodies to peripheral nerve in American trypanosomiasis (Chagas' disease).

E L Khoury, V Ritacco, P M Cossio, R P Laguens, A Szarfman, C Diez, R M Arana
PMCID: PMC1537703  PMID: 111884

Abstract

An antibody reacting with Schwann sheaths of myelinated somatic and unmyelinated autonomic peripheral nerve was found in sixty-one out of seventy-one chronic, and nine out of ten acute, Chagas' disease sera. Indirect immunofluorescence (IFL) was carried out on rat, mouse and human somatic nerves and rat sympathetic nerves with initial serum dilutions of 1 : 10, and the staining reached a final titre of 1 : 320 in some cases. The antibodies fixed complement and were absorbed out by lyophilized epimastigotes of T. cruzi. Lipid extraction of the tissue sections enhanced the staining of myelinated nerve, whereas unfixed unmyelinated sympathetic nerve was strongly reactive. Central nervous tissue did not display any positive staining on neurons, glial cells or periaxonal sheaths. Furthermore, by using a double-labelled IFL technique, it was possible to show that a rabbit antiserum raised against guinea-pig spinal cord and the chagasic anti-nerve antibodies reacted with different structures in the rat sciatic nerve. These findings suggest that the reactive antigen(s) could be located on Schwann cells. The majority, but not all, of the chagasic individuals with anti-nerve antibodies also showed the sarcolemmal and endothelial staining (EVI) previously described in Chagas' disease. The possible recognition of Schwann cell antigens by circulating antibodies in Chagas' disease could be relevant, since an autonomic denervation has been postulated as a pathogenic mechanism of cardiomyopathy and megaviscera in this condition.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLERAND C. D., YAHR M. D. GAMMA-GLOBULIN AFFINITY FOR NORMAL HUMAN TISSUE OF THE CENTRAL NERVOUS SYSTEM. Science. 1964 May 29;144(3622):1141–1147. doi: 10.1126/science.144.3622.1141. [DOI] [PubMed] [Google Scholar]
  2. Aarli J. A., Aparicio S. R., Lumsden C. E., Tönder O. Binding of normal human IgG to myelin sheaths, glia and neurons. Immunology. 1975 Jan;28(1):171–185. [PMC free article] [PubMed] [Google Scholar]
  3. Abramsky O., Lisak R. P., Silberberg D. H., Pleasure D. E. Antibodies to oligodendroglia in patients with multiple sclerosis. N Engl J Med. 1977 Dec 1;297(22):1207–1211. doi: 10.1056/NEJM197712012972204. [DOI] [PubMed] [Google Scholar]
  4. Alvarez M., Cerisola J. A., Rohwedder R. W. Test de inmunofluorescencia para diagnóstico de la enfermedad de Chagas. Bol Chil Parasitol. 1968 Jan-Jun;23(1):4–8. [PubMed] [Google Scholar]
  5. Beutner E. H., Witebsky E., Djanian A. Y. Serologic studies of myasthenia gravis. Ann N Y Acad Sci. 1965 Jun 30;124(2):709–729. doi: 10.1111/j.1749-6632.1965.tb18997.x. [DOI] [PubMed] [Google Scholar]
  6. Cossio P. M., Cornejo L., Herrera M., Vasquez C., Arana R. M. Skeletal and heart muscle antibodies in myasthenia gravis. A new immunofluorescence pattern. Biomedicine. 1973 Jun 20;19(6):261–266. [PubMed] [Google Scholar]
  7. Cossio P. M., Diez C., Szarfman A., Kreutzer E., Candiolo B., Arana R. M. Chagasic cardiopathy. Demonstration of a serum gamma globulin factor which reacts with endocardium and vascular structures. Circulation. 1974 Jan;49(1):13–21. doi: 10.1161/01.cir.49.1.13. [DOI] [PubMed] [Google Scholar]
  8. Cossio P. M., Laguens R. P., Diez C., Szarfman A., Segal A., Arana R. M. Chagasic cardiopathy. Antibodies reacting with plasma membrane of striated muscle and endothelial cells. Circulation. 1974 Dec;50(6):1252–1259. doi: 10.1161/01.cir.50.6.1252. [DOI] [PubMed] [Google Scholar]
  9. Edgington T. S., Dalessio D. J. The assessment by immunofluorescence methods of humoral anti-myelin antibodies in man. J Immunol. 1970 Jul;105(1):248–255. [PubMed] [Google Scholar]
  10. Hoff R., Teixeira R. S., Carvalho J. S., Mott K. E. Trypanosoma cruzi in the cerebrospinal fluid during the acute stage of Chagas' disease. N Engl J Med. 1978 Mar 16;298(11):604–606. doi: 10.1056/NEJM197803162981106. [DOI] [PubMed] [Google Scholar]
  11. Hübsch R. M., Sulzer A. J., Kagan I. G. Evaluation of an autoimmune type antibody in the sera of patients with Chagas' disease. J Parasitol. 1976 Aug;62(4):523–527. [PubMed] [Google Scholar]
  12. KOBERLE F., NADOR E. Etiologia e patogenia do megaesôfago no Brasil. Rev Paul Med. 1955 Dec;47(6):643–661. [PubMed] [Google Scholar]
  13. MAEKELT G. A. [The complement fixation reaction in Chagas' disease]. Z Tropenmed Parasitol. 1960 Aug;11:152–186. [PubMed] [Google Scholar]
  14. MOTT K. E., HAGSTROM J. W. THE PATHOLOGIC LESIONS OF THE CARDIAC AUTONOMIC NERVOUS SYSTEM IN CHRONIC CHAGAS' MYOCARDITIS. Circulation. 1965 Feb;31:273–286. doi: 10.1161/01.cir.31.2.273. [DOI] [PubMed] [Google Scholar]
  15. Nairn R. C. Standardization in immunofluorescence. Clin Exp Immunol. 1968 Jul;3(6):465–476. [PMC free article] [PubMed] [Google Scholar]
  16. Szarfman A., Otatti L., Schmuñis G. A., Vilches A. M. A simple method for the detection of human congenital Chagas' disease. J Parasitol. 1973 Aug;59(4):723–723. [PubMed] [Google Scholar]
  17. Teixeira A. R., Teixeira M. L., Santos-Buch C. A. The immunology of experimental Chagas' disease. IV. Production of lesions in rabbits similar to those of chronic Chagas' disease in man. Am J Pathol. 1975 Jul;80(1):163–180. [PMC free article] [PubMed] [Google Scholar]
  18. Tse K. S., Arbesman C. E., Tomasi T. B., Jr, Tourville D. Demonstration of antimyelin antibodies by immunofluorescence in Guillain-Barré syndrome. Clin Exp Immunol. 1971 Jun;8(6):881–887. [PMC free article] [PubMed] [Google Scholar]
  19. Vattuone N. H., Yanovsky J. F. Trypanosoma cruzi: agglutination activity of enzyme-treated epimastigotes. Exp Parasitol. 1971 Dec;30(3):349–355. doi: 10.1016/0014-4894(71)90098-1. [DOI] [PubMed] [Google Scholar]

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