Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1979 Aug;37(2):267–275.

Monoclonal IgG1 immunoglobulinaemia with strictly pH dependent cryoprecipitability.

Y Aizawa, Z A Zawadzki
PMCID: PMC1537821  PMID: 40721

Abstract

A monoclonal IgG1 lambda protein with peculiar cryoprecipitable characteristics was found in the serum of a patient with advanced multiple myeloma. The cryoprecipitate was observed only when the serum was exposed to the air. Further studies defined this phenomenon as being strictly pH-dependent. No antiglobulin activity (rheumatoid factor) was detected in the cryoglobulin. The isoelctric focussing of isolated cryoglobulin showed four distinct fractions around the pH 7.8 zone, which was comparable to the pH at which the cryoprecipitate began to form. Electrostatic bonds probably contribute to the mechanism involved in the formation of cryoprecipitate in this case.

Full text

PDF
267

Images in this article

269FIG. 1
on p.269

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bobrzecka K., Janicki K., Konieczny L. Cold aggregation properties of some macroglobulins. Clin Chim Acta. 1969 Dec;26(3):580–582. doi: 10.1016/0009-8981(69)90091-6. [DOI] [PubMed] [Google Scholar]
  2. Brouet J. C., Clauvel J. P., Danon F., Klein M., Seligmann M. Biologic and clinical significance of cryoglobulins. A report of 86 cases. Am J Med. 1974 Nov;57(5):775–788. doi: 10.1016/0002-9343(74)90852-3. [DOI] [PubMed] [Google Scholar]
  3. FUDENBERG H. H., DREWS G., NISONOFF A. SEROLOGIC DEMONSTRATION OF DUAL SPECIFICITY OF RABBIT BIVALENT HYBRID ANTIBODY. J Exp Med. 1964 Jan 1;119:151–166. doi: 10.1084/jem.119.1.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Grey H. M., Kohler P. F., Terry W. D., Franklin E. C. Human monoclonal gamma G-cryoglobulins with anti-gamma-globulin activity. J Clin Invest. 1968 Aug;47(8):1875–1884. doi: 10.1172/JCI105878. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hay F. C., Nineham L. J., Roitt I. M. Routine assay for detection of IgG and IgM antiglobulins in seronegative and seropositive rheumatoid arthritis. Br Med J. 1975 Jul 26;3(5977):203–204. doi: 10.1136/bmj.3.5977.203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. JANDL J. H., SIMMONS R. L. The agglutination and sensitization of red cells by metallic cations: interactions between multivalent metals and the red-cell membrane. Br J Haematol. 1957 Jan;3(1):19–38. doi: 10.1111/j.1365-2141.1957.tb05768.x. [DOI] [PubMed] [Google Scholar]
  7. MULLER-EBERHARD H. J., KUNKEL H. G. The carbohydrate of gamma-globulin and myeloma proteins. J Exp Med. 1956 Aug 1;104(2):253–269. doi: 10.1084/jem.104.2.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Meltzer M., Franklin E. C. Cryoglobulinemia--a study of twenty-nine patients. I. IgG and IgM cryoglobulins and factors affecting cryoprecipitability. Am J Med. 1966 Jun;40(6):828–836. doi: 10.1016/0002-9343(66)90199-9. [DOI] [PubMed] [Google Scholar]
  9. Rimington C. Seromucoid and the bound carbohydrate of the serum proteins. Biochem J. 1940 Jun;34(6):931–940. doi: 10.1042/bj0340931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Saha A., Edwards M. A., Sargent A. U., Rose B. Mechanism of cryo-precipitation. I. Characteristics of a human cryoglobulin. Immunochemistry. 1968 Jul;5(4):341–356. doi: 10.1016/0019-2791(68)90130-4. [DOI] [PubMed] [Google Scholar]
  11. Saulk P. H., Clem W. Studies on the cryoprecipitation of a human IGG3 cryoglobulin: the effects of temperature-induced comformational changes on the primary interaction. Immunochemistry. 1975 Jan;12(1):29–37. doi: 10.1016/0019-2791(75)90046-4. [DOI] [PubMed] [Google Scholar]
  12. WEIMER H. E., MOSHIN J. R. Serum glyco protein concentrations in experimental tuberculosis of guinea pigs. Am Rev Tuberc. 1953 Oct;68(4):594–602. doi: 10.1164/art.1953.68.4.594. [DOI] [PubMed] [Google Scholar]
  13. WINZLER R. J. Determination of serum glycoproteins. Methods Biochem Anal. 1955;2:279–311. doi: 10.1002/9780470110188.ch10. [DOI] [PubMed] [Google Scholar]
  14. Wrigley C. W. Analytical fractionation of plant and animal proteins by gel electrofocusing. J Chromatogr. 1968 Aug 27;36(3):362–365. doi: 10.1016/s0021-9673(01)92959-0. [DOI] [PubMed] [Google Scholar]
  15. Zinneman H. H., Levi D., Seal U. S. On the nature of cryoglobulins. J Immunol. 1968 Mar;100(3):594–603. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES