Abstract
Human blood B-lymphocytes were co-cultured with in vitro irradiated allogeneic or autologous T-lymphocytes in the presence of pokeweed mitogen (PWM). The production of IgG, IgM and IgA, as assessed by the enzyme-linked immunosorbent assay (ELISA) was increased 2--7 times, as compared to values obtained with non-irradiated T-lymphocytes. It is suggested that the increase of Ig production is due to the selective radiosensitivity of T-lymphocytes with suppressor function.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baral E., Blomgren H. Response of human lymphocytes to mitogenic stimuli after irradiation in vitro. Acta Radiol Ther Phys Biol. 1976 Apr;15(2):149–161. doi: 10.3109/02841867609132716. [DOI] [PubMed] [Google Scholar]
- Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
- Gupta S., Good R. A. Subpopulations of human T lymphocytes. II. Effect of thymopoietin, corticosteroids, and irradiation. Cell Immunol. 1977 Nov;34(1):10–18. doi: 10.1016/0008-8749(77)90224-6. [DOI] [PubMed] [Google Scholar]
- Moretta L., Webb S. R., Grossi C. E., Lydyard P. M., Cooper M. D. Functional analysis of two human T-cell subpopulations: help and suppression of B-cell responses by T cells bearing receptors for IgM or IgG. J Exp Med. 1977 Jul 1;146(1):184–200. doi: 10.1084/jem.146.1.184. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Penhale W. J., Farmer A., Irvine W. J. Thyroiditis in T cell-depleted rats. Influence of strain, radiation dose, adjuvants and antilymphocyte serum. Clin Exp Immunol. 1975 Sep;21(3):362–375. [PMC free article] [PubMed] [Google Scholar]
- Petrini B., Wasserman J., Blomgren H., Baral E. Blood lymphocyte subpopulations in breast cancer patients following radiotherapy. Clin Exp Immunol. 1977 Jul;29(1):36–42. [PMC free article] [PubMed] [Google Scholar]
- Siegal F. P., Siegal M. Enhancement by irradiated T cells of human plasma cell production: dissection of helper and suppressor functions in vitro. J Immunol. 1977 Feb;118(2):642–647. [PubMed] [Google Scholar]