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. 1974 Oct;18(2):213–224.

Immunological and virological studies of cultured labial biopsy cells from patients with Sjögren's syndrome

Natalie E Cremer, T E Daniels, L S Oshiro, F Marcus, R Claypool, R A Sylvester, N Talal
PMCID: PMC1537885  PMID: 4468195

Abstract

Labial salivary gland tissues from twenty-five patients were cultured in vitro for virus studies and for use as target cells in cellular and antibody-mediated cytotoxicity assays. Fourteen patients had definite Sjögren's Syndrome (SS), four had possible SS and seven did not have SS. No evidence for the presence of a virus in the cultured cells or after chemical treatment of the cultured cells was obtained. Tubuloreticular structures were present in three of the original biopsies but were not seen in the corresponding cultured cells, although in two of these cell lines rare bundles of intranuclear microfibrils occurred. The significance of these structures is unknown. Autologous serum and autologous lymphocytes were not cytotoxic for the cultured cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albegger K. W., Auböck L. Elektronenmikroskopischer Nachweis "virusähnlicher" Einschlusskörper bei myoepithelialer Sialadenitis im Rahmen des "Sjögrens"-Syndroms. Arch Klin Exp Ohren Nasen Kehlkopfheilkd. 1972;203(2):153–165. [PubMed] [Google Scholar]
  2. Berry H., Bacon P. A., Davis J. D. Cell-mediated immunity in Sjögren's syndrome. Ann Rheum Dis. 1972 Jul;31(4):298–302. doi: 10.1136/ard.31.4.298. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bunim J. J. The frequent occurrence of hypergammaglobulinemia and multiple tissue antibodies in Sjögren's syndrome. Ann N Y Acad Sci. 1965 Jun 30;124(2):852–859. doi: 10.1111/j.1749-6632.1965.tb19007.x. [DOI] [PubMed] [Google Scholar]
  4. Calder B. A., Penhale W. J., Barnes E. W., Irvine W. J. Cytotoxic lymphocytes in Hashimoto thyroiditis. An in vitro assay system using 51 Cr-labelled chicken red blood cells coated with thyroglobulin. Clin Exp Immunol. 1973 May;14(1):19–23. [PMC free article] [PubMed] [Google Scholar]
  5. Chisholm D. M., Mason D. K. Labial salivary gland biopsy in Sjögren's disease. J Clin Pathol. 1968 Sep;21(5):656–660. doi: 10.1136/jcp.21.5.656. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chisholm D. M., Waterhouse J. P., Mason D. K. Lymphocytic sialadenitis in the major and minor glands: a correlation in postmortem subjects. J Clin Pathol. 1970 Nov;23(8):690–694. doi: 10.1136/jcp.23.8.690. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dawkins R. L., Mastaglia F. L. Cell-mediated cytotoxicity to muscle in polymyositis. Effect of immunosuppression. N Engl J Med. 1973 Mar 1;288(9):434–438. doi: 10.1056/NEJM197303012880903. [DOI] [PubMed] [Google Scholar]
  8. Feldman M., Cohen I. R., Wekerle H. T-cell mediated immunity in vitro: an analysis of antigen recognition and target cell lysis. Transplant Rev. 1972;12:57–90. doi: 10.1111/j.1600-065x.1972.tb00053.x. [DOI] [PubMed] [Google Scholar]
  9. Ginsburg H. Graft versus host reaction in tissue culture. I. Lysis of monolayers of embryo mouse cells from strains differing in the H-2 histocompatibility locus by rat lymphocytes sensitized in vitro. Immunology. 1968 May;14(5):621–635. [PMC free article] [PubMed] [Google Scholar]
  10. Györkey F., Sinkovics J. G. Microtubules of systemic lupus erythematosus. Lancet. 1971 Jan 16;1(7690):131–132. doi: 10.1016/s0140-6736(71)90859-2. [DOI] [PubMed] [Google Scholar]
  11. Karlsson U. Three-dimensional studies of neurons in the lateral geniculate nucleus of the rat. I. Organelle organization in the perikaryon and its proximal branches. J Ultrastruct Res. 1966 Dec;16(5):429–481. doi: 10.1016/s0022-5320(66)80001-1. [DOI] [PubMed] [Google Scholar]
  12. Leventhal B. G., Waldorf D. S., Talal N. Impaired Lymphocyte Transformation and Delayed Hypersensitivity in Sjögren's Syndrome. J Clin Invest. 1967 Aug;46(8):1338–1345. doi: 10.1172/JCI105626. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MacSween R. N., Goudie R. B., Anderson J. R., Armstrong E., Murray M. A., Mason D. K., Jasani M. K., Boyle J. A., Buchanan W. W., Williamson J. Occurrence of antibody to salivary duct epithelium in Sjogren's disease, rheumatoid arthritis, and other arthritides. A clinical and laboratory study. Ann Rheum Dis. 1967 Sep;26(5):402–411. doi: 10.1136/ard.26.5.402. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Magalhães M. M. Intranuclear bodies in cells of rabbit and rat retina. Exp Cell Res. 1967 Sep;47(3):628–632. doi: 10.1016/0014-4827(67)90022-5. [DOI] [PubMed] [Google Scholar]
  15. Podleski W. K. Cytotoxic lymphocytes in Hashimoto's thyroiditis. Clin Exp Immunol. 1972 Aug;11(4):543–548. [PMC free article] [PubMed] [Google Scholar]
  16. SIEGESMUND K. A., DUTTA C. R., FOX C. A. THE ULTRASTRUCTURE OF THE INTRANUCLEAR RODLET IN CERTAIN NERVE CELLS. J Anat. 1964 Jan;98:93–97. [PMC free article] [PubMed] [Google Scholar]
  17. Schaff Z., Heine U., Dalton A. J. Ultramorphological and ultracytochemical studies on tubuloreticular structures in lymphoid cells. Cancer Res. 1972 Dec;32(12):2696–2706. [PubMed] [Google Scholar]
  18. Shearn M. A., Tu W. H., Stephens B. G., Lee J. C. Virus-like structure in Sjögren's syndrome. Lancet. 1970 Mar 14;1(7646):568–569. doi: 10.1016/s0140-6736(70)90804-4. [DOI] [PubMed] [Google Scholar]
  19. Stewart S. E., Kasnic G., Jr, Draycott C., Ben T. Activation of viruses in human tumors by 5-iododeoxyuridine and dimethyl sulfoxide. Science. 1972 Jan 14;175(4018):198–199. doi: 10.1126/science.175.4018.198. [DOI] [PubMed] [Google Scholar]
  20. TALAL N., BUNIM J. J. THE DEVELOPMENT OF MALIGNANT LYMPHOMA IN THE COURSE OF SJOEGREN'S SYNDROME. Am J Med. 1964 Apr;36:529–540. doi: 10.1016/0002-9343(64)90101-9. [DOI] [PubMed] [Google Scholar]
  21. Talal N. Sjögren's syndrome, lymphoproliferation, and renal tubular acidosis. Ann Intern Med. 1971 Apr;74(4):633–634. doi: 10.7326/0003-4819-74-4-633. [DOI] [PubMed] [Google Scholar]
  22. Talal N., Sylvester R. A., Daniels T. E., Greenspan J. S., Williams R. C., Jr T and B lymphocytes in peripheral blood and tissue lesions in Sjögren's syndrome. J Clin Invest. 1974 Jan;53(1):180–189. doi: 10.1172/JCI107536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Whaley K., Glen A. C., MacSween R. N., Deodhar S., Dick W. C., Nuki G., Williamson J., Buchanan W. W. Immunological responses in Sjogren's syndrome and rheumatoid arthritis. Clin Exp Immunol. 1971 Dec;9(6):721–732. [PMC free article] [PubMed] [Google Scholar]

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