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. 1974 Oct;18(2):273–282.

Inhibition by C3 fragments of C3-dependent rosette formation and antigen-induced lymphocyte transformation

M B Pepys, A E Butterworth
PMCID: PMC1537889  PMID: 4619599

Abstract

The interaction in vitro between human lymphocytes and fixed C3 was inhibited by fluid phase C3b and C3c prepared by trypsinization of purified human C3. These C3 fragments suppressed in vitro transformation of human lymphocytes by specific antigens, PPD and Candida albicans somatic antigen, but not by phytohaemagglutinin, a non-specific mitogen. These observations indicate the form of C3 which interacts with complement receptor lymphocytes and suggest that this interaction may play a part in lymphocyte activation by antigen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bokisch V. A., Müller-Eberhard H. J., Cochrane C. G. Isolation of a fragment (C3a) of the third component of human complement containing anaphylatoxin and chemotactic activity and description of an anaphylatoxin inactivator of human serum. J Exp Med. 1969 May 1;129(5):1109–1130. doi: 10.1084/jem.129.5.1109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Eden A., Bianco C., Nussenzweig V., Mayer M. M. C3 split products inhibit the binding of antigen-antibody-complement complexes to B lymphocytes. J Immunol. 1973 May;110(5):1452–1453. [PubMed] [Google Scholar]
  3. Feldmann M., Pepys M. B. Role of C3 in in vitro lymphocyte cooperation. Nature. 1974 May 10;249(453):159–161. doi: 10.1038/249159a0. [DOI] [PubMed] [Google Scholar]
  4. Geha R. S., Schneeberger E., Rosen F. S., Merler E. Interaction of human thymus-derived and non-thymus-derived lymphocytes in vitro. Induction of proliferation and antibody synthesis in B lymphocytes by a soluble factor released from antigen-stimulated T lymphocytes. J Exp Med. 1973 Nov 1;138(5):1230–1247. doi: 10.1084/jem.138.5.1230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hallberg T., Gurner B. W., Coombs R. R. Opsonic adherence of sensitized ox red cells to human lymphocytes as measured by rosette formation. Int Arch Allergy Appl Immunol. 1973;44(4):500–513. doi: 10.1159/000230956. [DOI] [PubMed] [Google Scholar]
  6. Janossy G., Humphrey J. H., Pepys M. B., Greaves M. F. Complement independence of stimulation of mouse splenic B lymphocytes by mitogens. Nat New Biol. 1973 Sep 26;245(143):108–112. doi: 10.1038/newbio245108a0. [DOI] [PubMed] [Google Scholar]
  7. Jondal M., Holm G., Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972 Aug 1;136(2):207–215. doi: 10.1084/jem.136.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LACHMANN P. J., MULLER-EBERHARD H. J., KUNKEL H. G., PARONETTO F. The localization of in vivo bound complement in tissue section. J Exp Med. 1962 Jan 1;115:63–82. doi: 10.1084/jem.115.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lachmann P. J., Liske R. The preparation and properties of alexinated intermediates that react with conglutinin. II. Equine, rabbit and human complement. Immunology. 1966 Sep;11(3):255–262. [PMC free article] [PubMed] [Google Scholar]
  10. Michlmayr G., Huber H. Receptor sites for complement on certain human peripheral blood lymphocytes. J Immunol. 1970 Sep;105(3):670–676. [PubMed] [Google Scholar]
  11. Moorhead J. W., Walters C. S., Claman H. N. Immunologic reactions to haptens on autologous carriers. I. Participation of both thymus-derived and bone marrow-derived cells in the secondary in vitro response. J Exp Med. 1973 Feb 1;137(2):411–423. doi: 10.1084/jem.137.2.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Müllerèberhard H. J., Dalmasso A. P., Calcott M. A. The reaction mechanism of beta-1C-globulin (C'3) in immune hemolysis. J Exp Med. 1966 Jan 1;123(1):33–54. doi: 10.1084/jem.123.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. PEPYS J. The relationship of nonspecific and specific factors in the tuberculin reaction: a review. Am Rev Tuberc. 1955 Jan;71(1):49–73. doi: 10.1164/artpd.1955.71.1.49. [DOI] [PubMed] [Google Scholar]
  14. Pepys M. B. Role of complement in induction of antibody production in vivo. Effect of cobra factor and other C3-reactive agents on thymus-dependent and thymus-independent antibody responses. J Exp Med. 1974 Jul 1;140(1):126–145. doi: 10.1084/jem.140.1.126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pepys M. B. Role of complement in induction of the allergic response. Nat New Biol. 1972 May 31;237(74):157–159. doi: 10.1038/newbio237157a0. [DOI] [PubMed] [Google Scholar]
  16. Pincus S., Bianco C., Nussenzweig V. Increased proportion of complement-receptor lymphocytes in the peripheral blood of patients with chronic lymphocytic leukemia. Blood. 1972 Sep;40(3):303–310. [PubMed] [Google Scholar]
  17. Ross G. D., Polley M. J., Rabellino E. M., Grey H. M. Two different complement receptors on human lymphocytes. One specific for C3b and one specific for C3b inactivator-cleaved C3b. J Exp Med. 1973 Oct 1;138(4):798–811. doi: 10.1084/jem.138.4.798. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ross G. D., Rabellino E. M., Polley M. J., Grey H. M. Combined studies of complement receptor and surface immunoglobulin-bearing cells and sheep erythrocyte rosette-forming cells in normal and leukemic human lymphocytes. J Clin Invest. 1973 Feb;52(2):377–385. doi: 10.1172/JCI107194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stjernswärd J., Jondal M., Vánky F., Wigzell H., Sealy R. Lymphopenia and change in distribution of human B and T lymphocytes in peripheral blood induced by irradiation for mammary carcinoma. Lancet. 1972 Jun 24;1(7765):1352–1356. doi: 10.1016/s0140-6736(72)91091-4. [DOI] [PubMed] [Google Scholar]
  20. Vischer T. L., Jaquet C. Effect of antibodies against immunoglobulins and the theta antigen on the specific and non-specific stimulation of mouse spleen cells in vitro. Immunology. 1972 Feb;22(2):259–266. [PMC free article] [PubMed] [Google Scholar]
  21. West C., Davis N. C., Forristal J., Herbst J., Spitzer R. Antigenic determinants of human beta-1c and beta-1g-globulins. J Immunol. 1966 Apr;96(4):650–658. [PubMed] [Google Scholar]

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