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. 1974 Oct;18(2):177–186.

Rosette formation by mouse lymphocytes. II. T-cell specificities in a CRL subpopulation

A Arnaiz-Villena, Maria I C Gyöngyössy, J H L Playfair
PMCID: PMC1537890  PMID: 4219768

Abstract

Mouse spleen and lymph node lymphocytes with receptors for complement (CRL) were prepared by a standard rosetting method and the identity of the central lymphocyte studied by indirect immunofluorescence with heterologous antisera specific for immunoglobulin and for T cells. About 30% of CRL were shown to be T cells and the remainder immunoglobulin-bearing (B) cells.

In thymus-less `nude' mice a population of immunoglobulin-negative, non-T cell CRL was found.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bianco C., Nussenzweig V. Theta-bearing and complement-receptor lymphocytes are distinct populations of cells. Science. 1971 Jul 9;173(3992):154–156. doi: 10.1126/science.173.3992.154. [DOI] [PubMed] [Google Scholar]
  2. Bianco C., Patrick R., Nussenzweig V. A population of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. I. Separation and characterization. J Exp Med. 1970 Oct 1;132(4):702–720. doi: 10.1084/jem.132.4.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. COHN Z. A., WIENER E. THE PARTICULATE HYDROLASES OF MACROPHAGES. I. COMPARATIVE ENZYMOLOGY, ISOLATION, AND PROPERTIES. J Exp Med. 1963 Dec 1;118:991–1008. doi: 10.1084/jem.118.6.991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dukor P., Bianco C., Nussenzweig V. Tissue localization of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. Proc Natl Acad Sci U S A. 1970 Oct;67(2):991–997. doi: 10.1073/pnas.67.2.991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eden A., Bianco C., Nussenzweig Mechanism of binding of soluble immune complexes to lymphocytes. Cell Immunol. 1973 Jun;7(3):459–473. doi: 10.1016/0008-8749(73)90210-4. [DOI] [PubMed] [Google Scholar]
  6. Eden A., Bianco C., Nussenzweig V. A population of lymphocytes bearing a membrane receptor antigen-antibody-complement complexes. II. Specific isolation. Cell Immunol. 1971 Dec;2(6):658–669. doi: 10.1016/0008-8749(71)90013-x. [DOI] [PubMed] [Google Scholar]
  7. Gyöngyössy M. I., Playfair J. H. Indirect immunofluorescence of mouse thymus-derived cells using heterologous anti-brain serum. Cell Immunol. 1973 Apr;7(1):118–123. doi: 10.1016/0008-8749(73)90187-1. [DOI] [PubMed] [Google Scholar]
  8. Gyöngyössy M. I., Playfair J. H. Rosette formation by mouse lymphocytes. I. Demonstration by indirect immunofluorescence of T cells binding sheep erythrocytes. Clin Exp Immunol. 1974 Oct;18(2):169–176. [PMC free article] [PubMed] [Google Scholar]
  9. Henson P. M. The adherence of leucocytes and platelets induced by fixed IgG antibody or complement. Immunology. 1969 Jan;16(1):107–121. [PMC free article] [PubMed] [Google Scholar]
  10. Huber H., Polley M. J., Linscott W. D., Fudenberg H. H., Müller-Eberhard H. J. Human monocytes: distinct receptor sites for the third component of complement and for immunoglobulin G. Science. 1968 Dec 13;162(3859):1281–1283. doi: 10.1126/science.162.3859.1281. [DOI] [PubMed] [Google Scholar]
  11. Jondal M., Holm G., Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972 Aug 1;136(2):207–215. doi: 10.1084/jem.136.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Komuro K., Boyse E. A. Induction of T lymphocytes from precursor cells in vitro by a product of the thymus. J Exp Med. 1973 Aug 1;138(2):479–482. doi: 10.1084/jem.138.2.479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lay W. H., Nussenzweig V. Ca++-dependent binding of antigen-19 S antibody complexes to macrophages. J Immunol. 1969 May;102(5):1172–1178. [PubMed] [Google Scholar]
  14. Lay W. H., Nussenzweig V. Receptors for complement of leukocytes. J Exp Med. 1968 Nov 1;128(5):991–1009. doi: 10.1084/jem.128.5.991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Loor F., Kindred B. Differentiation of T-cell precursors in nude mice demonstrated by immunofluorescence of T-cell membrane markers. J Exp Med. 1973 Nov 1;138(5):1044–1055. doi: 10.1084/jem.138.5.1044. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pincus S., Bianco C., Nussenzweig V. Increased proportion of complement-receptor lymphocytes in the peripheral blood of patients with chronic lymphocytic leukemia. Blood. 1972 Sep;40(3):303–310. [PubMed] [Google Scholar]
  17. Playfair J. H. Strain differences in the immune response of mice. I. The neonatal response to sheep red cells. Immunology. 1968 Jul;15(1):35–50. [PMC free article] [PubMed] [Google Scholar]
  18. Ross G. D., Rabellino E. M., Polley M. J., Grey H. M. Combined studies of complement receptor and surface immunoglobulin-bearing cells and sheep erythrocyte rosette-forming cells in normal and leukemic human lymphocytes. J Clin Invest. 1973 Feb;52(2):377–385. doi: 10.1172/JCI107194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schlossman S. F., Hudson L. Specific purification of lymphocyte populations on a digestible immunoabsorbent. J Immunol. 1973 Jan;110(1):313–319. [PubMed] [Google Scholar]
  20. Silveira N. P., Mendes N. F., Tolnai M. E. Tissue localization of two populations of human lymphocytes distinguished by membrane receptors. J Immunol. 1972 May;108(5):1456–1460. [PubMed] [Google Scholar]
  21. Soteriades-Vlachos C., Gyöngyössy M. I., Playfair J. H. Rosette formation by mouse lymphocytes. III. Receptors for immunoglobulin on normal and activated T cells. Clin Exp Immunol. 1974 Oct;18(2):187–192. [PMC free article] [PubMed] [Google Scholar]
  22. Uhr J. W. Passive sensitization of lymphocytes and macrophages by antigen-antibody complexes. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1599–1606. doi: 10.1073/pnas.54.6.1599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wortis H. H., Nehlsen S., Owen J. J. Abnormal development of the thymus in "nude" mice. J Exp Med. 1971 Sep 1;134(3 Pt 1):681–692. doi: 10.1084/jem.134.3.681. [DOI] [PMC free article] [PubMed] [Google Scholar]

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