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. 1974 Oct;18(2):169–176.

Rosette formation by mouse lymphocytes. I. Demonstration by indirect immunofluorescence of T cells binding sheep erythrocytes

Maria I C Gyöngyössy, J H L Playfair
PMCID: PMC1537897  PMID: 4549624

Abstract

A method is described for indirect immunofluorescent staining of mouse T cells forming rosettes, involving fixation of rosettes by glutaraldehyde and staining with a rabbit anti-mouse brain serum absorbed so as to be specific for T cells. Using this technique, it was shown that about 44% of spleen cells forming rosettes with sheep erythrocytes both in non-immunized and immunized mice, are T cells. The number of T rosette-forming cells increased about ten-fold after immunization with sheep erythrocytes, but only when serum antibody was detectable.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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