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. 1980 Jan;39(1):215–221.

Measurement of lymphocyte traffic with indium-111.

T Issekutz, W Chin, J B Hay
PMCID: PMC1537949  PMID: 7389193

Abstract

This study was designed to assess the use of 111indium as a radioactive marker for the investigation of lymphocyte recirculation in the sheep. Lymphocytes were collected from sheep with indwelling catheters in the efferent lymphatic ducts of peripheral lymph nodes and labelled with 111In-oxine or Na2 51CrO at doses of 10 microCi and 50 microCi/10(8) cells respectively. After intravenous injection the lymphocyte specific activity (c.p.m./10(7) cells) in blood and lymph was measured for several days. The maximum specific activity in efferent lymph was twelve-fold greater with 111In than with 51Cr-labelled cells. The kinetics of lymphocyte traffic as measured in double labelling experiments was very similar. The modal transit time was 21.6 hr with each isotope. The recovery of 111In-labelled cells was not significantly different from cells labelled with 51Cr. In vivo viability of the labelled cells was further supported by the normal proliferative response observed with 111In-labelled lymphocytes compared to unlabelled cells in the normal lymphocyte transfer reaction. In conclusion, 111In-oxine is an excellent radioactive label for lymphocytes in the sheep. Because of its high counting efficiency and cell labelling characteristics one can label as few as 10 million lymphocytes, or a subpopulation of cells, and assess their recirculation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cahill R. N., Frost H., Trnka Z. The effects of antigen on the migration of recirculating lymphocytes through single lymph nodes. J Exp Med. 1976 Apr 1;143(4):870–888. doi: 10.1084/jem.143.4.870. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ford W. L. Lymphocyte migration and immune responses. Prog Allergy. 1975;19:1–59. doi: 10.1159/000313381. [DOI] [PubMed] [Google Scholar]
  3. Ford W. L., Simmonds S. J. The tempo of lymphocyte recirculation from blood to lymph in the rat. Cell Tissue Kinet. 1972 Mar;5(2):175–189. doi: 10.1111/j.1365-2184.1972.tb01014.x. [DOI] [PubMed] [Google Scholar]
  4. Frost P., Smith J., Frost H. The radiolabeling of lymphocytes and tumor cells with 111indium. Proc Soc Exp Biol Med. 1978 Jan;157(1):61–65. doi: 10.3181/00379727-157-39991. [DOI] [PubMed] [Google Scholar]
  5. GOWANS J. L. The recirculation of lymphocytes from blood to lymph in the rat. J Physiol. 1959 Apr 23;146(1):54–69. doi: 10.1113/jphysiol.1959.sp006177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HALL J. G., MORRIS B. The lymph-borne cells of the immune response. Q J Exp Physiol Cogn Med Sci. 1963 Jul;48:235–247. doi: 10.1113/expphysiol.1963.sp001660. [DOI] [PubMed] [Google Scholar]
  7. HALL J. G., MORRIS B. The output of cells in lymph from the popliteal node of sheep. Q J Exp Physiol Cogn Med Sci. 1962 Oct;47:360–369. doi: 10.1113/expphysiol.1962.sp001620. [DOI] [PubMed] [Google Scholar]
  8. Hall J., Scollay R., Smith M. Studies on the lymphocytes of sheep. I. Recirculation of lymphocytes through peripheral lymph nodes and tissues. Eur J Immunol. 1976 Feb;6(2):117–120. doi: 10.1002/eji.1830060209. [DOI] [PubMed] [Google Scholar]
  9. Hersey P. The separation and 51 chromium labeling of human lymphocytes with in vivo studies of survival and migration. Blood. 1971 Sep;38(3):360–371. [PubMed] [Google Scholar]
  10. Lafferty K. J., Jones M. A. Reactions of the graft versus host (GVH) type. Aust J Exp Biol Med Sci. 1969 Feb;47(1):17–54. doi: 10.1038/icb.1969.3. [DOI] [PubMed] [Google Scholar]
  11. Rannie G. H., Donald K. J. Estimation of the migration of thoracic duct lymphocytes to non-lymphoid tissues. A comparison of the distribution of radioactivity at intervals following i.v. transfusion of cells labelled with 3H, 14C, 75Se, 99mTc, 125I and 51Cr in the rat. Cell Tissue Kinet. 1977 Nov;10(6):523–541. [PubMed] [Google Scholar]
  12. Rannie G. H., Thakur M. L., Ford W. L. An experimental comparison of radioactive labels with potential application to lymphocyte migration studies in patients. Clin Exp Immunol. 1977 Sep;29(3):509–514. [PMC free article] [PubMed] [Google Scholar]
  13. Thakur M. L., Coleman R. E., Welch M. J. Indium-111-labeled leukocytes for the localization of abscesses: preparation, analysis, tissue distribution, and comparison with gallium-67 citrate in dogs. J Lab Clin Med. 1977 Jan;89(1):217–228. [PubMed] [Google Scholar]

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