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. 1975 Jan;19(1):1–9.

Antibody-dependent lymphocyte killer function in human immunodeficiency diseases.

G S Rachelefsky, P R McConnachie, A J Ammann, P I Terasaki, E R Stiehm
PMCID: PMC1538024  PMID: 1081921

Abstract

Antibody-dependent cell immunity to the lymphocyte system (ABCIL) has been shown to be a function of a non-thymus-processed cell in the experimental animal. To evaluate its role in the human and to assess its clinical usefulness, we assessed ABCIL in twenty-five patients with various immunodeficiency (ID) syndromes. Our technique measures the lysis of 51Cr-labelled normal human lymphocytes coated with HL-A-specific antibody. Cytotoxicity is expressed as a percentage of 51Cr released after subtracting the spontaneous target cell release. Mean values in normals are 20+/-2 (s.e.). The ten patients with AB deficiency had a mean ABCIL of 7-9+/-2 (Pless than0-01). All eight patients with cellular ID had normal ABCIL (18+/-2), while the ten patients with combined ID had variable results. Effector cell function in the ABCIL test correlated (r=0-74; Pless than0-05) with the percentage of B cells in the peripheral blood. No correlation was found between ABCIL function and serum immunoglobulin levels or rosette-forming cells in the peripheral blood. There is a function for B lymphocytes other than as a precursor of antibody-synthesizing cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Basten A., Miller J. F., Sprent J., Pye J. A receptor for antibody on B lymphocytes. I. Method of detection and functional significance. J Exp Med. 1972 Mar 1;135(3):610–626. doi: 10.1084/jem.135.3.610. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brunner K. T., Mauel J., Cerottini J. C., Chapuis B. Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs. Immunology. 1968 Feb;14(2):181–196. [PMC free article] [PubMed] [Google Scholar]
  3. Carlsson H. E., Hammarström S., Lanteli M., Perlmann P. Antibody-induced destruction of colon antigen-coated chicken erythrocytes by normal human lymphocytes. Eur J Immunol. 1971 Aug;1(4):281–285. doi: 10.1002/eji.1830010413. [DOI] [PubMed] [Google Scholar]
  4. Dickler H. B., Kunkel H. G. Interaction of aggregated -globulin with B lymphocytes. J Exp Med. 1972 Jul 1;136(1):191–196. doi: 10.1084/jem.136.1.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Greaves M. F. Biological effects of anti-immunoglobulins: evidence for immunoglobulin receptors on 'T' and 'B' lymphocytes. Transplant Rev. 1970;5:45–75. doi: 10.1111/j.1600-065x.1970.tb00356.x. [DOI] [PubMed] [Google Scholar]
  6. Lawlor G. J., Jr, Stiehm E. R., Kaplan M. S., Sengar D. P., Terasaki P. I. Phytohemagglutinin (PHA) skin test in the diagnosis of cellular immunodeficiency. J Allergy Clin Immunol. 1973 Jul;52(1):31–37. doi: 10.1016/0091-6749(73)90118-8. [DOI] [PubMed] [Google Scholar]
  7. MOELLER E. CONTACT-INDUCED CYTOTOXICITY BY LYMPHOID CELLS CONTAINING FOREIGN ISOANTIGENS. Science. 1965 Feb 19;147(3660):873–879. doi: 10.1126/science.147.3660.873. [DOI] [PubMed] [Google Scholar]
  8. MacLennan I. C. Competition for receptors for immunoglobulin on cytotoxic lymphocytes. Clin Exp Immunol. 1972 Feb;10(2):275–283. [PMC free article] [PubMed] [Google Scholar]
  9. MacLennan I. C., Harding B. Failure of certain cytotoxic lymphocytes to respond mitotically to phytohaemagglutinin. Nature. 1970 Sep 19;227(5264):1246–1248. doi: 10.1038/2271246a0. [DOI] [PubMed] [Google Scholar]
  10. McConnachie P. R., Dossetor J. B. Antibody-mediated cell-dependent immunity in human lymphocytes. Tissue Antigens. 1973;3(4):303–308. doi: 10.1111/j.1399-0039.1973.tb01007.x. [DOI] [PubMed] [Google Scholar]
  11. McConnachie P. R., Rachelefsky G., Stiehm E. R., Terasaki P. I. Antibody-dependent lymphocyte killer function and age. Pediatrics. 1973 Dec;52(6):795–800. [PubMed] [Google Scholar]
  12. Mittal K. K., Mickey M. R., Singal D. P., Terasaki P. I. Serotyping for homotransplantation. 18. Refinement of microdroplet lymphocyte cytotoxicity test. Transplantation. 1968 Nov;6(8):913–927. doi: 10.1097/00007890-196811000-00006. [DOI] [PubMed] [Google Scholar]
  13. Möller G., Svehag S. E. Specificiy of lymphocyte-mediated cytotoxicity induced by in vitro antibody-coated target cells. Cell Immunol. 1972 May;4(1):1–19. doi: 10.1016/0008-8749(72)90001-9. [DOI] [PubMed] [Google Scholar]
  14. Perlmann P., Holm G. Cytotoxic effects of lymphoid cells in vitro. Adv Immunol. 1969;11:117–193. doi: 10.1016/s0065-2776(08)60479-4. [DOI] [PubMed] [Google Scholar]
  15. Perlmann P., Perlmann H. Contactual lysis of antibody-coated chicken erythrocytes by purified lymphocytes. Cell Immunol. 1970 Sep;1(3):300–315. doi: 10.1016/0008-8749(70)90051-1. [DOI] [PubMed] [Google Scholar]
  16. Sengar D. P., Terasaki P. I. A semimicro mixed leukocyte culture test. Transplantation. 1971 Mar;11(3):260–267. doi: 10.1097/00007890-197103000-00004. [DOI] [PubMed] [Google Scholar]
  17. Stiehm E. R., Fudenberg H. H. Serum levels of immune globulins in health and disease: a survey. Pediatrics. 1966 May;37(5):715–727. [PubMed] [Google Scholar]
  18. Van Boxel J. A., Stobo J. D., Paul W. E., Green I. Antibody-dependent lymphoid cell-mediated cytotoxicity: no requirement for thymus-derived lymphocytes. Science. 1972 Jan 14;175(4018):194–196. doi: 10.1126/science.175.4018.194. [DOI] [PubMed] [Google Scholar]
  19. Wisloff F., Froland S. S. Antibody-dependent lymphocyte-mediated cytotoxicity in man: no requirement for lymphocytes with membrane-bound immunoglobulin. Scand J Immunol. 1973;2(2):151–157. doi: 10.1111/j.1365-3083.1973.tb02026.x. [DOI] [PubMed] [Google Scholar]
  20. Wybran J., Levin A. S., Spitler L. E., Fudenberg H. H. Rosette-forming cells, immunologic deficiency diseases and transfer factor. N Engl J Med. 1973 Apr 5;288(14):710–713. doi: 10.1056/NEJM197304052881405. [DOI] [PubMed] [Google Scholar]

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