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. 1980 Feb;39(2):538–543.

Hidden anti-nuclear antibodies in rheumatic diseases.

P S Mach, D Piatier, A Le Go, F Delbarre
PMCID: PMC1538074  PMID: 6993073

Abstract

Hidden anti-nuclear antibodies are demonstrated by immunofluorescence using smears of rat nuclei as substrate and rat liver section technique when sera are incubated with penicillamine. The non-detection of hidden anti-nuclear antibodies by tissue sections in the absence of a splitting agent may be due to the formation of high molecular weight complexes between rheumatoid factors and anti-nuclear antibodies. These high molecular weight complexes containing anti-nuclear antibodies do not have access to tissue nuclear antigens, but can react directly with free nuclei. It is postulated that anti-nuclear antibodies may represent the early pathway of both rheumatoid arthritis and connective tissue diseases. The demonstration of hidden anti-nuclear antibodies in seropositive sera indicates that rheumatoid factors may have a protective effect. It may explain dissimilarities observed in the clinico-immunological profile of rheumatoid arthritis and systemic lupus erythematosus. The splitting effect of penicillamine observed in vitro may be similar in vivo. It can explain clinical improvement and immunological side effects observed in rheumatoid arthritis patients treated with this drug.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bacon P. A., Tribe C. R., Mackenzie J. C., Verrier-Jones J., Cumming R. H., Amer B. Penicillamine nephropathy in rheumatoid arthritis. A clinical, pathological and immunological study. Q J Med. 1976 Oct;45(180):661–684. [PubMed] [Google Scholar]
  2. Barnett E. V., Bienenstock J., Bloch K. J. Antinuclear factors in synovia. Possible participants in the rheumatoid inclusion body. JAMA. 1966 Oct 10;198(2):143–148. [PubMed] [Google Scholar]
  3. Blomjous F. J., Feltkamp-Vroom T. M. Hidden anti-nuclear antibodies in sero-negative systemic lupus erythematosus patients and in NZB and (NZB x NZW) F 1 mice. Eur J Immunol. 1971 Nov;1(5):396–398. doi: 10.1002/eji.1830010519. [DOI] [PubMed] [Google Scholar]
  4. Crouzet J., Camus J. P., Leca A. P., Guillien P., Lièvre J. A. Lupus induit par la D-pénicillamine au cours du traitement de la polyarthrite rhumatoïde. Deux observation et étude immunologique systématique au cours de ce traitment. Ann Med Interne (Paris) 1974 Jan;125(1):71–79. [PubMed] [Google Scholar]
  5. Dissanayake S., Hay F. C., Roitt I. M. The binding constants of IgM rheumatoid factors and their univalent fragments for native and aggregated human IgG;. Immunology. 1977 Mar;32(3):309–318. [PMC free article] [PubMed] [Google Scholar]
  6. Koffler D., Schur P. H., Kunkel H. G. Immunological studies concerning the nephritis of systemic lupus erythematosus. J Exp Med. 1967 Oct 1;126(4):607–624. doi: 10.1084/jem.126.4.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Krishnan C., Kaplan M. H. Immunopathologic studies of systemic lupus erythematosus. II. Antinuclear reaction of gamma-globulin eluted from homogenates and isolated glomeruli of kidneys from patients with lupus nephritis. J Clin Invest. 1967 Apr;46(4):569–579. doi: 10.1172/JCI105558. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mach P. S., Kahan A., de Mayo C., Delbarre F. Etude cytochimique des ragocytes synoviaux. C R Seances Soc Biol Fil. 1968 Oct 19;162(3):604–609. [PubMed] [Google Scholar]
  9. Mach P. S., Piatier D., Le Go A., Delbarre F. Interactions between IgM antiglobulins and IgG antinuclear antibodies. Some aspects of D-penicillamine activities. Clin Exp Immunol. 1979 May;36(2):311–316. [PMC free article] [PubMed] [Google Scholar]
  10. Mannik M., Arend M. P., Hall A. P., Gilliland B. C. Studies on antigen-antibody complexes. I. Elimination of soluble complexes from rabbit circulation. J Exp Med. 1971 Apr 1;133(4):713–739. doi: 10.1084/jem.133.4.713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nies K. M., Louie J. S. Impaired immunoglobulin synthesis by peripheral blood lymphocytes in systemic lupus erythematosus. Arthritis Rheum. 1978 Jan-Feb;21(1):51–57. doi: 10.1002/art.1780210109. [DOI] [PubMed] [Google Scholar]
  12. Winfield J. B., Koffler D., Kunkel H. G. Role of DNA-anti-DNA complexes in the immunopathogenesis of tissue injury in systemic lupus erythematosus. Scand J Rheumatol Suppl. 1975;11:59–64. doi: 10.3109/03009747509095630. [DOI] [PubMed] [Google Scholar]

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