Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1975 Mar;19(3):407–416.

The leucocyte migration technique as an assay of presensitization to human transplantation antigens.

A J D'Apice, B D Tait, P J Morris
PMCID: PMC1538146  PMID: 1204243

Abstract

An assay of cell-mediated immunity, the leucocyte migration technique (LMT), has been compared with the commonly used assay of humoral immunity to transplantation antigens, the microlymphocytotoxicity test. Five recently multiply transfused patients and nineteen haemodialysis patients who had been potentially immunized against transplantation antigens were studied by both tests. All five transfused patients gave positive LMT results while only two gave a positive serological test. Sixteen out of nineteen dialysis patients gave positive LMT results compared with nine with known anti-HL-A antibodies and four showing positive serological direct cross-matches. There was no correlation between cell-mediated and humoral immunity as detected by these methods, although there was a tendency, which was not absolute, for positive direct cross-matches between patients' sera and the panel's lymphocytes, to be associated with positive LMT results. There was a significant correlation (P less than 0-05) between the number of blood transfusions received by the dialysis patients and the detection of cytotoxic antibodies but not with the LMT results. It is felt that some of these positive LMT results are due to reactivity with non-HL-A antigens, and that false negative results also occur. For this reason the technique is not of great value in determining presensitization against HL-A before transplantation.

Full text

PDF
407

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clausen J. E., Soborg M. In vitro detection of tuberculin hypersensitivity in man. Specific migration inhibition of white blood cells from tuberculin-positive persons. Acta Med Scand. 1969 Sep;186(3):227–230. [PubMed] [Google Scholar]
  2. Cochrum K. C., Davis W. C., Kountz S. L., Fudenberg H. H. Renal autograft rejection initiated by passive transfer of immune plasma. Transplant Proc. 1969 Mar;1(1):301–304. [PubMed] [Google Scholar]
  3. Cooper M. D., Raymond D. A., Peterson R. D., South M. A., Good R. A. The functions of the thymus system and the bursa system in the chicken. J Exp Med. 1966 Jan 1;123(1):75–102. doi: 10.1084/jem.123.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. D'Apice A. J., Morris P. J. The role of antibody-dependent cell-mediated cytotoxicity in renal allograft rejection. Transplantation. 1974 Jul;18(1):20–26. doi: 10.1097/00007890-197407000-00004. [DOI] [PubMed] [Google Scholar]
  5. Dossetor J. B., Olson L. A. Evidence of latent sensitization to HL-A antigens. Transplantation. 1972 Jun;13(6):576–579. doi: 10.1097/00007890-197206000-00004. [DOI] [PubMed] [Google Scholar]
  6. Eddleston A. L., Smith M. G., Mitchell C. G., Williams R. Application of the leucocyte migration test to the diagnosis of rejection after liver transplantation. Transplantation. 1971 Jul;12(1):11–17. doi: 10.1097/00007890-197107000-00002. [DOI] [PubMed] [Google Scholar]
  7. Eddleston A. L., Williams R., Calne R. Y. Cellular immune response during rejection of a liver transplant in man. Nature. 1969 May 17;222(5194):674–675. doi: 10.1038/222674a0. [DOI] [PubMed] [Google Scholar]
  8. Falk R. E., Collste L., Möller G. In vitro detection of transplantation immunity: the inhibition of migration of immune spleen cells and peripheral blood leukocytes by specific antigen. J Immunol. 1970 May;104(5):1287–1292. [PubMed] [Google Scholar]
  9. Falk R. E., Guttmann R. D., Falk J. A., Beaudoin J. G., Deveber G., Morehouse D. D., Wilson D. R. Assessment of the cellular immune response to HL-A antigens in human renal allograft recipients. Clin Exp Immunol. 1973 May;14(1):47–56. [PMC free article] [PubMed] [Google Scholar]
  10. Falk R. E., Thorsby E., Möller E., Möller G. In vitro assay of cell-mediated immunity: the inhibition of migration of sensitized human lymphocytes by HL-A antigens. Clin Exp Immunol. 1970 Apr;6(4):445–455. [PMC free article] [PubMed] [Google Scholar]
  11. Garovoy M. R., Franco V., Zschaeck D., Carpenter C. B., Strom T. B., Merrill J. P. Derect lymphocyte-mediated cytotoxicity as an assay of presensitisation. Lancet. 1973 Mar 17;1(7803):573–576. doi: 10.1016/s0140-6736(73)90717-4. [DOI] [PubMed] [Google Scholar]
  12. Girard J. P. Valeur du test d'inhibition de la migration des cellules lymphoïdes, in vitro, appliqué au diagnostic des allergies médicamenteuses. Schweiz Med Wochenschr. 1968 Oct 19;98(42):1655–1656. [PubMed] [Google Scholar]
  13. KRETSCHMER R. R., PEREZ-TAMAYO R. The role of humoral antibodies in rejection of skin homografts in rabbits. II. Passive transfer of transplantation immunity by sensitized lymph node cells within diffusion chambers. J Exp Med. 1962 Dec 1;116:879–896. doi: 10.1084/jem.116.6.879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kissmeyer-Nielsen F., Olsen S., Petersen V. P., Fjeldborg O. Hyperacute rejection of kidney allografts, associated with pre-existing humoral antibodies against donor cells. Lancet. 1966 Sep 24;2(7465):662–665. doi: 10.1016/s0140-6736(66)92829-7. [DOI] [PubMed] [Google Scholar]
  15. MITCHISON N. A. Passive transfer of transplantation immunity. Proc R Soc Lond B Biol Sci. 1954 Feb 18;142(906):72–87. doi: 10.1098/rspb.1954.0007. [DOI] [PubMed] [Google Scholar]
  16. MITCHISON N. A. Studies on the immunological response to foreign tumor transplants in the mouse. I. The role of lymph node cells in conferring immunity by adoptive transfer. J Exp Med. 1955 Aug 1;102(2):157–177. doi: 10.1084/jem.102.2.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mittal K. K., Mickey M. R., Singal D. P., Terasaki P. I. Serotyping for homotransplantation. 18. Refinement of microdroplet lymphocyte cytotoxicity test. Transplantation. 1968 Nov;6(8):913–927. doi: 10.1097/00007890-196811000-00006. [DOI] [PubMed] [Google Scholar]
  18. Morris P. J., Dumble L. J. Use of lymphoblastoid cell-lines to determine responsiveness in cadaveric renal transplantation. Lancet. 1973 Jul 7;2(7819):16–18. doi: 10.1016/s0140-6736(73)91948-x. [DOI] [PubMed] [Google Scholar]
  19. Morris P. J., Kincaid-Smith P., McKenzie I. F., Marshall V. C., Ting A. Leukocyte antigens in renal transplantation. 7. Immediate renal allograft rejection with a negative cross match. Med J Aust. 1969 Aug 23;2(8):379–382. [PubMed] [Google Scholar]
  20. Myburgh J. A., Cohen I., Gecelter L., Meyers A. M., Abrahams C., Furman K. I., Goldberg B., van Blerk P. J. Hyperacute rejection in human-kidney allografts-shwartzman or arthus reaction? N Engl J Med. 1969 Jul 17;281(3):131–135. doi: 10.1056/NEJM196907172810305. [DOI] [PubMed] [Google Scholar]
  21. Opelz G., Mickey M. R., Terasaki P. I. Identification of unresponsive kidney-transplant recipients. Lancet. 1972 Apr 22;1(7756):868–871. doi: 10.1016/s0140-6736(72)90739-8. [DOI] [PubMed] [Google Scholar]
  22. Patel R., Terasaki P. I. Significance of the positive crossmatch test in kidney transplantation. N Engl J Med. 1969 Apr 3;280(14):735–739. doi: 10.1056/NEJM196904032801401. [DOI] [PubMed] [Google Scholar]
  23. Smith M. G., Eddleston A. L., Dominguez J. A., Evans D. B., Bewick M., Williams R. Changes in leucocyte migration after renal transplantation. Br Med J. 1969 Nov 1;4(5678):275–278. doi: 10.1136/bmj.4.5678.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Soborg M., Bendixen G. Human lymphocyte migration as a parameter of hypersensitivity. Acta Med Scand. 1967 Feb;181(2):247–256. doi: 10.1111/j.0954-6820.1967.tb07255.x. [DOI] [PubMed] [Google Scholar]
  25. Soborg M. In citro migration of peripheral human leucocytes in cellular hypersensitivity. Acta Med Scand. 1968 Jul-Aug;184(1-2):135–139. [PubMed] [Google Scholar]
  26. Soborg M. In vitro detection of cellular hypersensitivity in man. Specific migration inhibition of white blood cells from brucella-positive persons. Acta Med Scand. 1967 Aug;182(2):167–174. [PubMed] [Google Scholar]
  27. Soborg M. Interaction of human peripheral lymphocytes and granulocytes in the migration-inhibition reaction. Acta Med Scand. 1969 Mar;185(3):221–226. [PubMed] [Google Scholar]
  28. Starzl T. E., Lerner R. A., Dixon F. J., Groth C. G., Brettschneider L., Terasaki P. I. Shwartzman reaction after human renal homotransplantation. N Engl J Med. 1968 Mar 21;278(12):642–648. doi: 10.1056/NEJM196803212781202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Terasaki P. I., Kreisler M., Mickey R. M. Presensitization and kidney transplant failures. Postgrad Med J. 1971 Feb;47(544):89–100. [PMC free article] [PubMed] [Google Scholar]
  30. Weeke E., Weeke B., Bendixen G. Organ-specific, antirenal cellular hypersensitivity after kidney transplantation. Acta Med Scand. 1970 Oct;188(4):307–315. doi: 10.1111/j.0954-6820.1970.tb08043.x. [DOI] [PubMed] [Google Scholar]
  31. Williams G. M., Hume D. M., Hudson R. P., Jr, Morris P. J., Kano K., Milgrom F. "Hyperacute" renal-homograft rejection in man. N Engl J Med. 1968 Sep 19;279(12):611–618. doi: 10.1056/NEJM196809192791201. [DOI] [PubMed] [Google Scholar]
  32. Wood R. F., Gray A. C., Briggs J. D., Bell P. R. The prediction of acute rejection in human renal transplantation using the leucocyte migration test. Transplantation. 1973 Jul;16(1):41–45. doi: 10.1097/00007890-197307000-00008. [DOI] [PubMed] [Google Scholar]
  33. Wunderlich J. R., Rogentine G. N., Jr, Yankee R. A. Rapid in vitro detection of cellular immunity in man against freshly explanted allogeneic cells. Transplantation. 1972 Jan;13(1):31–37. doi: 10.1097/00007890-197201000-00008. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES