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. 1975 May;20(2):227–237.

Immunoglobulin E (IgE) and IgE-containing cells in human gastrointestinal fluids and tissues.

W R Brown, B K Borthistle, S T Chen
PMCID: PMC1538192  PMID: 813925

Abstract

Human gastric, small intestinal, colonic and rectal mucosae were examined for IgE-containing cells by single- and double-antibody immunofluorescence techniques, and IgE in intesinal fluids was measured by a double-antibody radioimmunoassay. IgE-containing cells were identified in all tissue specimens and comprised about 2% of all immunoglobulin-containing cells. Although less numerous than cells containing IgA, IgM or IgG, they were remarkably numerous in relation to the concentration of IgE in serum (about 0-001% of total immunoglobulin). IgE immunocytes were significantly more numerous in stomach and proximal small bowel than in colon and rectum, and were very numerous at bases of gastric and duodenal peptic ulcers. Measurable IgE was found in seventy-eight of eighty-five (92%) intestinal fluids. Sucrose gradient ultracentrifugation analysis of four of the fluids revealed that the immunologically reactive IgE was largely in fractions corresponding to molecules of lower molecular weight than that of albumin, which suggests that IgE in gut contents is degraded by proteolytic enzymes. The presence of IgE-forming cells in gastrointestinal tissues, and IgE or a fragment of IgE in intestinal fluids, suggests that IgE antibodies are available for participation in local reaginic-type reactions in the gut.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barratt T. M., Turner M. W., Johansson S. G. Urinary excretion of immunoglobulin E in the nephrotic syndrome and atopic eczema. Lancet. 1971 Aug 21;2(7721):402–403. doi: 10.1016/s0140-6736(71)90116-4. [DOI] [PubMed] [Google Scholar]
  2. Brauninger G. E., Centifanto Y. M. Immunoglobulin E in human tears. Am J Ophthalmol. 1971 Sep;72(3):558–561. doi: 10.1016/0002-9394(71)90853-1. [DOI] [PubMed] [Google Scholar]
  3. Brown W. R., Lansford C. L., Hornbrook M. Serum immunoglobulin E (IGE) concentrations in patients with gastrointestinal disorders. Am J Dig Dis. 1973 Aug;18(8):641–645. doi: 10.1007/BF01072034. [DOI] [PubMed] [Google Scholar]
  4. Brown W. R., Newcomb R. W., Ishizaka K. Proteolytic degradation of exocrine and serum immunoglobulins. J Clin Invest. 1970 Jul;49(7):1374–1380. doi: 10.1172/JCI106354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cebra J. J., Goldstein G. Chromatographic purification of tetramethylrhodamine-immune globulin conjugates and their use in the cellular localization of rabbit gamma-globulin polypeptide chains. J Immunol. 1965 Aug;95(2):230–245. [PubMed] [Google Scholar]
  6. Chen S. T. Cellular sites of immunoglobulins. II. The relative proportions of mucosal cells containing IgG, IgA, and IgM, and light polypeptide chains of kappa and lambda immunoglobulin in human appendices. Acta Pathol Jpn. 1971 Feb;21(1):67–83. [PubMed] [Google Scholar]
  7. Dandliker W. B., De Saussure V. A. Antibody purification at neutral pH utilizing immunospecific adsorbents. Immunochemistry. 1968 Jul;5(4):357–365. doi: 10.1016/0019-2791(68)90131-6. [DOI] [PubMed] [Google Scholar]
  8. Deuschl H., Johansson S. G. Immunoglobulins in tracheo-bronchial secretion with special reference to IgE. Clin Exp Immunol. 1974 Mar;16(3):401–412. [PMC free article] [PubMed] [Google Scholar]
  9. Gleich G. J., Averbeck A. K., Swedlund H. A. Measurement of IgE in normal and allergic serum by radioimmunoassay. J Lab Clin Med. 1971 Apr;77(4):690–698. [PubMed] [Google Scholar]
  10. Heiner D. C., Rose B. Elevated levels of gamma-E (IgE) in conditions other than classical allergy. J Allergy. 1970 Jan;45(1):30–42. doi: 10.1016/0021-8707(70)90014-6. [DOI] [PubMed] [Google Scholar]
  11. Hobbs J. R., Hepner G. W., Douglas A. P., Crabbé P. A., Johansson S. G. Immunological mystery of coeliac disease. Lancet. 1969 Sep 20;2(7621):649–650. doi: 10.1016/s0140-6736(69)90362-6. [DOI] [PubMed] [Google Scholar]
  12. Ishizaka K., Ishizaka T., Lee E. H. Biologic function of the Fc fragments of E myeloma protein. Immunochemistry. 1970 Aug;7(8):687–702. doi: 10.1016/0019-2791(70)90175-8. [DOI] [PubMed] [Google Scholar]
  13. Ishizaka K., Newcomb R. W. Presence of gammaE in nasal washings and sputum from asthmatic patients. J Allergy. 1970 Oct;46(4):197–204. doi: 10.1016/0021-8707(70)90023-7. [DOI] [PubMed] [Google Scholar]
  14. Johansson S. G., Bennich H. Immunological studies of an atypical (myeloma) immunoglobulin. Immunology. 1967 Oct;13(4):381–394. [PMC free article] [PubMed] [Google Scholar]
  15. Johansson S. G., Mellbin T., Vahlquist B. Immunoglobulin levels in Ethiopian preschool children with special reference to high concentrations of immunoglobulin E (IgND). Lancet. 1968 May 25;1(7552):1118–1121. doi: 10.1016/s0140-6736(68)90187-6. [DOI] [PubMed] [Google Scholar]
  16. Klinman N. R., Taylor R. B. General methods for the study of cells and serum during the immune response: the response to dinitrophenyl in mice. Clin Exp Immunol. 1969 Apr;4(4):473–487. [PMC free article] [PubMed] [Google Scholar]
  17. Murray M. Secretory antibody and local immunity: effector mechanisms of immediate hypersensitivity at mucosal surfaces. Proc R Soc Med. 1972 Aug;65(8):659–662. [PMC free article] [PubMed] [Google Scholar]
  18. Newcomb R. W., Ishizaka K. Physicochemical and antigenic studies on human gamma E in respiratory fluid. J Immunol. 1970 Jul;105(1):85–89. [PubMed] [Google Scholar]
  19. Ogilvie B. M., Jones V. E. Parasitological review. Nippostrongylus brasiliensis: a review of immunity and host-parasite relationship in the rat. Exp Parasitol. 1971 Feb;29(1):138–177. doi: 10.1016/0014-4894(71)90021-x. [DOI] [PubMed] [Google Scholar]
  20. Ozkaragöz K., Smith H. J., Gökcen M. IgE levels in serum, saliva, and urine of normal individuals. Acta Allergol. 1972 Dec;27(5):392–396. doi: 10.1111/j.1398-9995.1972.tb01438.x. [DOI] [PubMed] [Google Scholar]
  21. Polmar S. H., Waldmann T. A., Terry W. D. A comparison of three radioimmunoassay techniques for the measurement of serum IgE. J Immunol. 1973 May;110(5):1253–1261. [PubMed] [Google Scholar]
  22. Rosenberg E. B., Polmar S. H., Whalen G. E. Increased circulating IgE in trichinosis. Ann Intern Med. 1971 Oct;75(4):575–578. doi: 10.7326/0003-4819-75-4-575. [DOI] [PubMed] [Google Scholar]
  23. Rosenberg E. B., Whalen G. E., Bennich H., Johansson S. G. Increased circulating IgE in a new parasitic disease--human intestinal capillariasis. N Engl J Med. 1970 Nov 19;283(21):1148–1149. doi: 10.1056/NEJM197011192832107. [DOI] [PubMed] [Google Scholar]
  24. Savilahti E. Immunoglobulin-containing cells in the intestinal mucosa and immunoglobulins in the intestinal juice in children. Clin Exp Immunol. 1972 Jul;11(3):415–425. [PMC free article] [PubMed] [Google Scholar]
  25. Stanworth D. R., Humphrey J. H., Bennich H., Johansson S. G. Inhibition of Prausnitz-Küstner reaction by proteolytic-cleavage fragments of a human myeloma protein of immunoglobulin class E. Lancet. 1968 Jul 6;2(7558):17–18. doi: 10.1016/s0140-6736(68)92889-4. [DOI] [PubMed] [Google Scholar]
  26. Tada T., Ishizaka K. Distribution of gamma E-forming cells in lymphoid tissues of the human and monkey. J Immunol. 1970 Feb;104(2):377–387. [PubMed] [Google Scholar]
  27. Waldman R. H., Virchow C., Rowe D. S. IgE levels in external secretions. Int Arch Allergy Appl Immunol. 1973;44(2):242–248. doi: 10.1159/000230933. [DOI] [PubMed] [Google Scholar]

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