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. 1975 May;20(2):265–272.

In vitro production of IgE by lymphocytes from a patient with hyperimmunoglobulinaemia E, eosinophilia and increased lymphocytes carrying surface IgE.

R Patterson, I M Suszko, C C Hsu, M Roberts, S H Oh
PMCID: PMC1538202  PMID: 1212809

Abstract

The peripheral blood lymphocytes of a patient with massive hyperimmunoglobulinaemia E were used for in vitro studies. The serum IgE ranged from 140,000-210,000 u/ml. Peripheral blood lymphocytes had approximately 7% of cells staining for surface IgE. When these cells were cultured in vitro, IgE was produced as measured by the double antibody radioimmunoassay technique. The total IgE produced ranged from 140 to 484 units per 24 hr in different cultures. IgE production was greatest in the first 24 hr of culture and declined progressively thereafter. Some cultures still had measurable IgE at 48 hr. If the lymphocytes staining for surface IgE were the cells producing the IgE, it was estimated that between 1-7 and 2-8 molecules per cell per second were produced. No definite effect of concanavalin A, pokeweed mitogen or phytohaemagglutinin on in vitro IgE production could be demonstrated under the conditions of these experiments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bazaral M., Hamburger R. N. Standardization and stability of immunoglobulin E (IgE). J Allergy Clin Immunol. 1972 Mar;49(3):189–191. doi: 10.1016/0091-6749(72)90113-3. [DOI] [PubMed] [Google Scholar]
  2. Bazaral M., Orgel H. A., Hamburger R. N. IgE levels in normal infants and mothers and an inheritance hypothesis. J Immunol. 1971 Sep;107(3):794–801. [PubMed] [Google Scholar]
  3. Cooper M. D., Lawton A. R., Kincade P. W. A two-stage model for development of antibody-producing cells. Clin Exp Immunol. 1972 May;11(1):143–149. [PMC free article] [PubMed] [Google Scholar]
  4. Douglas S. D., Fudenberg H. H. In vitro development of plasma cells from lymphocytes following pokeweed mitogen stimulation: a fine structural study. Exp Cell Res. 1969 Feb;54(2):277–279. doi: 10.1016/0014-4827(69)90252-3. [DOI] [PubMed] [Google Scholar]
  5. Forbes I. J., Lawton J. M. Autoradiographic analysis of proteins synthesized by human lymphocytes. I. Multiple myeloma and macroglobulinaemia. Evidence relevant to clonal selection theory. Australas Ann Med. 1969 Feb;18(1):12–18. doi: 10.1111/imj.1969.18.1.12. [DOI] [PubMed] [Google Scholar]
  6. Ghanta V. K., McGhee J. R., Hamlin N. M., Hiramoto R. N. Measurement of antibody release from single cells. 3. Rate of release of IgM from MOPC 104E plasmacytoma. J Immunol. 1974 Jan;112(1):266–270. [PubMed] [Google Scholar]
  7. Gleich G. J., Averbeck A. K., Swedlund H. A. Measurement of IgE in normal and allergic serum by radioimmunoassay. J Lab Clin Med. 1971 Apr;77(4):690–698. [PubMed] [Google Scholar]
  8. Greaves M. F., Roitt I. M. The effect of phytohaemagglutinin and other lymphocyte mitogens on immunoglobulin synthesis by human peripheral blood lymphocytes in vitro. Clin Exp Immunol. 1968 Jun;3(5):393–412. [PMC free article] [PubMed] [Google Scholar]
  9. Ishizaka K., Ishizaka T. The significance of immunoglobulin E in reaginic hypersensitivity. Ann Allergy. 1970 May;28(5):189–202. [PubMed] [Google Scholar]
  10. Moore R. D., Schoenberg M. D. Restimulation of antibody synthesis by antigen in cultures of lymphocytes. Nature. 1968 Jul 20;219(5151):297–298. doi: 10.1038/219297a0. [DOI] [PubMed] [Google Scholar]
  11. PATTERSON R., SUSZKO I. M., PRUZANSKY J. J. IN VITRO PRODUCTION OF CHICKEN GLOBULINS AND PRECIPITATING ANTIBODY. Immunology. 1964 Jul;7:440–448. [PMC free article] [PubMed] [Google Scholar]
  12. Wall J. R., Good B. F., Forbes I. J., Hetzel B. S. Demonstration of the production of the long acting thyroid stimulator (LATS) by peripheral lymphocytes cultured in vitro. Clin Exp Immunol. 1973 Aug;14(4):555–561. [PMC free article] [PubMed] [Google Scholar]
  13. Walters C. S., Wigzell H. Demonstration of heavy and light chain antigenic determinants on the cell-bound receptor for antigen. Similarities between membrane-attached and humoral antibodies produced by the same cell. J Exp Med. 1970 Dec 1;132(6):1233–1249. doi: 10.1084/jem.132.6.1233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wu L. Y., Lawton A. R., Cooper M. D. Differentiation capacity of cultured B lymphocytes from immunodeficient patients. J Clin Invest. 1973 Dec;52(12):3180–3189. doi: 10.1172/JCI107518. [DOI] [PMC free article] [PubMed] [Google Scholar]

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