Abstract
A lymphoblastoid cell line of human origin (`Moore') showed short or long microvilli when stained in a smeared state by indirect immunofluorescence using human serum containing smooth muscle antibodies (SMA), horse anti-lymphocyte immunoglobulin (ALI) or rabbit anti-species serum. A prerequisite for obtaining a positive reaction with SMA-positive serum was that a chelating agent was present in the suspending medium when preparing the smears. ALI and anti-species serum, however, stained the microvilli in the presence of calcium. These sera also stained live suspended lymphoblastoid cells, which SMA did not. The brush-border of monkey duodenal epithelial cells showed the same prerequisites for staining with SMA as the lymphoblastoid cells.
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Selected References
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- Biberfeld G., Fagraeus A., Lenkei R. Reaction of human smooth muscle antibody with thyroid cells. Clin Exp Immunol. 1974 Nov;18(3):371–377. [PMC free article] [PubMed] [Google Scholar]
- FORD C. E., HAMERTON J. L. A colchicine, hypotonic citrate, squash sequence for mammalian chromosomes. Stain Technol. 1956 Nov;31(6):247–251. doi: 10.3109/10520295609113814. [DOI] [PubMed] [Google Scholar]
- Fagraeus A., Espmark J. A., Jonsson J. Mixed haemadsorption: a mixed antiglobulin reaction applied to antigens on a glass surface. Preparation and evaluation of indicator red cells; survey of present applications. Immunology. 1965 Aug;9(2):161–175. [PMC free article] [PubMed] [Google Scholar]
- Fagraeus A., Lidman K., Biberfeld G. Reaction of human smooth muscle antibodies with human blood lymphocytes and lymphoid cell lines. Nature. 1974 Nov 15;252(5480):246–247. doi: 10.1038/252246a0. [DOI] [PubMed] [Google Scholar]
- Fagraeus A., The H., Biberfeld G. Reaction of human smooth muscle antibody with thymus medullary cells. Nat New Biol. 1973 Nov 28;246(152):113–115. doi: 10.1038/newbio246113a0. [DOI] [PubMed] [Google Scholar]
- Farrow L. J., Holborow E. J., Brighton W. D. Reaction of human smooth muscle antibody with liver cells. Nat New Biol. 1971 Aug 11;232(2):186–187. doi: 10.1038/newbio232186a0. [DOI] [PubMed] [Google Scholar]
- Gabbiani G., Ryan G. B., Lamelin J. P., Vassalli P., Majno G., Bouvier C. A., Cruchaud A., Lüscher E. F. Human smooth muscle autoantibody. Its identification as antiactin antibody and a study of its binding to "nonmuscular" cells. Am J Pathol. 1973 Sep;72(3):473–488. [PMC free article] [PubMed] [Google Scholar]
- Klein G., Bregula U., Wiener F., Harris H. The analysis of malignancy by cell fusion. I. Hybrids between tumour cells and L cell derivatives. J Cell Sci. 1971 May;8(3):659–672. doi: 10.1242/jcs.8.3.659. [DOI] [PubMed] [Google Scholar]
- Matter A., Lisowska-Bernstein B., Ryser J. E., Lamelin J. P., Vassalli P. Mouse thymus-independent and thymus-derived lymphoid cells. II. Ultrastructural studies. J Exp Med. 1972 Nov 1;136(5):1008–1030. doi: 10.1084/jem.136.5.1008. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Svehag S. E., Haxner P., Manhem L., Balner H., Sundqvist K., Schilling W. Further studies on antihuman thymocyte IgG. Antibody specificities, opsonin activity correlated to immunosuppression, lymphopenia, and hyporesponsiveness to horse IgG in primates. Transplantation. 1972 Jul;14(1):21–28. doi: 10.1097/00007890-197207000-00003. [DOI] [PubMed] [Google Scholar]
- Tilney L. G., Mooseker M. Actin in the brush-border of epithelial cells of the chicken intestine. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2611–2615. doi: 10.1073/pnas.68.10.2611. [DOI] [PMC free article] [PubMed] [Google Scholar]