Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1975 Jul;21(1):115–120.

Complement-induced mixed aggregation of T and B lymphocytes and myeloid cells and the effect of adjuvant.

A Arnaiz-Villena, I M Roitt
PMCID: PMC1538232  PMID: 171110

Abstract

Complement induces mixed aggregation of murine spleen cells bearing C3 receptors. In the present studies, we show with immunofluorescent techniques that these aggregates are formed by T and B lymphocytes and myeloid cells, B cells constituting the predominant component in normal mice. There are striking changes in the quantity and quality of the clumps after in vitro treatment with adjuvant, mostly due to an increase in macrophages and possibly in T cells. The possible meaning of these phenomena in T-cell-B-cell co-operation and in the mode of action of adjuvants is discussed.

Full text

PDF
115

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnaiz-Villena A., Gyöngyössy M. I., Playfair J. H. Rosette formation by mouse lymphocytes. II. T-cell specificities in a CRL subpopulation. Clin Exp Immunol. 1974 Oct;18(2):177–186. [PMC free article] [PubMed] [Google Scholar]
  2. Arnaiz-Villena A., Playfair J. H., Roitt I. M. C3 receptor. A marker of a thymus-dependent B-cell subpopulation. Clin Exp Immunol. 1975 May;20(2):375–378. [PMC free article] [PubMed] [Google Scholar]
  3. Bianchi F. B., Penfold P. L., Roitt I. M. Mitochondrial antibodies in primary biliary cirrhosis. V. Ultrastructural localization of the antigen to the inner mitochondrial membrane using a direct peroxidase conjugate. Br J Exp Pathol. 1973 Dec;54(6):652–657. [PMC free article] [PubMed] [Google Scholar]
  4. Bianco C., Patrick R., Nussenzweig V. A population of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. I. Separation and characterization. J Exp Med. 1970 Oct 1;132(4):702–720. doi: 10.1084/jem.132.4.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Feldmann M., Pepys M. B. Role of C3 in in vitro lymphocyte cooperation. Nature. 1974 May 10;249(453):159–161. doi: 10.1038/249159a0. [DOI] [PubMed] [Google Scholar]
  6. Gyöngyössy M. I., Playfair J. H. Indirect immunofluorescence of mouse thymus-derived cells using heterologous anti-brain serum. Cell Immunol. 1973 Apr;7(1):118–123. doi: 10.1016/0008-8749(73)90187-1. [DOI] [PubMed] [Google Scholar]
  7. Gyöngyössy M. I., Playfair J. H. Rosette formation by mouse lymphocytes. I. Demonstration by indirect immunofluorescence of T cells binding sheep erythrocytes. Clin Exp Immunol. 1974 Oct;18(2):169–176. [PMC free article] [PubMed] [Google Scholar]
  8. Hanifin J. M., Cline M. J. Human monocytes and macrophages. Interaction with antigen and lymphocytes. J Cell Biol. 1970 Jul;46(1):97–105. doi: 10.1083/jcb.46.1.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lay W. H., Nussenzweig V. Receptors for complement of leukocytes. J Exp Med. 1968 Nov 1;128(5):991–1009. doi: 10.1084/jem.128.5.991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Miller H. R., Avrameas S. Association between macrophages and specific antibody producing cells. Nat New Biol. 1971 Feb 10;229(6):184–185. doi: 10.1038/newbio229184a0. [DOI] [PubMed] [Google Scholar]
  11. Modolell M., Luckenbach G. A., Parant M., Munder P. G. The adjuvant activity of a mycobacterial water soluble adjuvant (WSA) in vitro. I. The requirement of macrophages. J Immunol. 1974 Jul;113(1):395–403. [PubMed] [Google Scholar]
  12. Mosier D. E. Cell interactions in the primary immune response in vitro: a requirement for specific cell clusters. J Exp Med. 1969 Feb 1;129(2):351–362. doi: 10.1084/jem.129.2.351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pepys M. B. Complement-mediated mixed aggregation of murine spleen cells. Nature. 1974 May 3;249(452):51–53. doi: 10.1038/249051a0. [DOI] [PubMed] [Google Scholar]
  14. Pepys M. B. Role of complement in induction of the allergic response. Nat New Biol. 1972 May 31;237(74):157–159. doi: 10.1038/newbio237157a0. [DOI] [PubMed] [Google Scholar]
  15. Robinson P. J., Roitt I. M. Identification of a population of mouse leukocytes using wheat germ agglutinin. Nature. 1974 Aug 9;250(5466):517–517. doi: 10.1038/250517a0. [DOI] [PubMed] [Google Scholar]
  16. Soteriades-Vlachos C., Gyöngyössy M. I., Playfair J. H. Rosette formation by mouse lymphocytes. III. Receptors for immunoglobulin on normal and activated T cells. Clin Exp Immunol. 1974 Oct;18(2):187–192. [PMC free article] [PubMed] [Google Scholar]
  17. Sulitzeanu D., Kleinman R., Benezra D., Gery I. Cellular interactions and the secondary response in vitro. Nat New Biol. 1971 Feb 24;229(8):254–255. doi: 10.1038/newbio229254a0. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES