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. 1975 Aug;21(2):226–235.

Cytotoxic lymphocytes from normal donors. A functional marker of human non-T lymphocytes.

H F Pross, M Jondal
PMCID: PMC1538269  PMID: 810282

Abstract

A phenomenon we have termed spontaneous lymphocyte-mediated cytotoxicity (SLMC) by non-thymus-derived lymphocytes from normal donors has been described. The phenomenon can be demonstrated using human and xenogeneic (mouse) cell lines as the target cell in a microplate 51Cr release assay which is simple and reproducible. In this paper, SLMC against xenogeneic targets has been evaluated as a potential marker of lumphocytotoxic function with respect to: (a) the nature of the target cell; (b) the variability of the cytotoxic function of lymphocytes from different donors, and from the same donor tested on different days; (c) the nature of the effector cell. Using buoyant density centrifugation of iron plus magnetpurified lymphocytes forming rosettes with sheep red blood cells (SRBC) (T-cells) or SRBC-rabbit 19S anti-SRBC-mouse complement (complement receptor lymphocyte), it has been demonstrated that the cytotoxic activity lies in the non-T-lymphocyte fraction, and is probably caused by the complement receptor-bearing lymphocyte. The potential usefulness of this phenomenon as a functional marker of non-T-LYMPHOCYTE CYTOTOXIC Ability, and for the assessment of serological factors ehich may affect this cytotoxicity, has been discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bertrams J., Kuwert E., Liedtke U. HL-A antigens and multiple sclerosis. Tissue Antigens. 1972;2(5):405–408. doi: 10.1111/j.1399-0039.1972.tb00060.x. [DOI] [PubMed] [Google Scholar]
  2. Bianco C., Patrick R., Nussenzweig V. A population of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. I. Separation and characterization. J Exp Med. 1970 Oct 1;132(4):702–720. doi: 10.1084/jem.132.4.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boyle W. An extension of the 51Cr-release assay for the estimation of mouse cytotoxins. Transplantation. 1968 Sep;6(6):761–764. doi: 10.1097/00007890-196809000-00002. [DOI] [PubMed] [Google Scholar]
  4. Cerottini J. C., Brunner K. T. Cell-mediated cytotoxicity, allograft rejection, and tumor immunity. Adv Immunol. 1974;18:67–132. doi: 10.1016/s0065-2776(08)60308-9. [DOI] [PubMed] [Google Scholar]
  5. DUNN T. B., POTTER M. A transplantable mast-cell neoplasm in the mouse. J Natl Cancer Inst. 1957 Apr;18(4):587–601. [PubMed] [Google Scholar]
  6. Jersild C., Svejgaard A., Fog T., Ammitzboll T. HL-A antigens and diseases. I. Multiple sclerosis. Tissue Antigens. 1973;3(4):243–250. doi: 10.1111/j.1399-0039.1973.tb01002.x. [DOI] [PubMed] [Google Scholar]
  7. Jondal M., Holm G., Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972 Aug 1;136(2):207–215. doi: 10.1084/jem.136.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jondal M., Klein G. Surface markers on human B and T lymphocytes. II. Presence of Epstein-Barr virus receptors on B lymphocytes. J Exp Med. 1973 Dec 1;138(6):1365–1378. doi: 10.1084/jem.138.6.1365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jondal M. Surface markers on human B and T lymphocytes. IV. Distribution of surface markers on resting and blast-transformed lymphocytes. Scand J Immunol. 1974;3(6):739–747. doi: 10.1111/j.1365-3083.1974.tb01309.x. [DOI] [PubMed] [Google Scholar]
  10. Jondal M., Wigzell H., Aiuti F. Human lymphocyte subpopulations: classification according to surface markers and-or functional characteristics. Transplant Rev. 1973;16:163–195. doi: 10.1111/j.1600-065x.1973.tb00120.x. [DOI] [PubMed] [Google Scholar]
  11. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  12. LANDY M., MICHAEL J. G., TRAPANI R. J., ACHINSTEIN B., WOODS M. W., SHEAR M. J. An antibody-complement system in normal serum lethal to mouse tumor cells. Cancer Res. 1960 Oct;20:1279–1291. [PubMed] [Google Scholar]
  13. Lozzio C. B., Lozzio B. B. Cytotoxicity of a factor isolated from human spleen. J Natl Cancer Inst. 1973 Feb;50(2):535–538. doi: 10.1093/jnci/50.2.535. [DOI] [PubMed] [Google Scholar]
  14. Lundgren G., Zukoski C. F., Möller G. Differential effects of human granulocytes and lymphocytes on human fibroblasts in vitro. Clin Exp Immunol. 1968 Oct;3(8):817–836. [PMC free article] [PubMed] [Google Scholar]
  15. Naito S., Namerow N., Mickey M. R., Terasaki P. I. Multiple sclerosis: association with HL-A3. Tissue Antigens. 1972;2(1):1–4. doi: 10.1111/j.1399-0039.1972.tb00111.x. [DOI] [PubMed] [Google Scholar]
  16. Petrányi G. G., Benczur M., Onody C. E., Hollán S. R. Letter: HL-A 3,7 and lymphocyte cytotoxic activity. Lancet. 1974 Apr 20;1(7860):736–736. doi: 10.1016/s0140-6736(74)92943-2. [DOI] [PubMed] [Google Scholar]
  17. Relations of HL-A and Rh systems to immune reactivity. Joint report of the results of 'HL-A and immune response' workshop, Budapest 1972. Vox Sang. 1974;26(5):470–482. [PubMed] [Google Scholar]
  18. Stathopoulos G., Elliott E. V. Formation of mouse or sheep red-blood-cell rosettes by lymphocytes from normal and leukaemic individuals. Lancet. 1974 Apr 6;1(7858):600–601. doi: 10.1016/s0140-6736(74)92655-5. [DOI] [PubMed] [Google Scholar]
  19. WIGZELL H. QUANTITATIVE TITRATIONS OF MOUSE H-2 ANTIBODIES USING CR-51-LABELLED TARGET CELLS. Transplantation. 1965 May;3:423–431. doi: 10.1097/00007890-196505000-00011. [DOI] [PubMed] [Google Scholar]

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