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. 1975 Sep;21(3):501–509.

Quantification of IgG subclasses in sera of normal adults and healthy children between 4 and 12 years of age.

M van der Giessen, E Rossouw, T A van Veen, E van Loghem, B J Zegers, P C Sander
PMCID: PMC1538307  PMID: 54236

Abstract

The concentration of the four subclasses of IgG was determined in sera of normal adults and healthy children between 4 and 12 years of age, using the radial immunodiffusion technique. A relation between the concentration of IgG subclasses and Gm type was studied in adults. No influence of Gm type on IgG1 concentration could be shown, except that the group of Gm(fb) individuals had a higher level than the others. The mean concentration of IgG2 was higher in sera positive for Gm(n) than in those lacking this genetic marker. High IgG3 concentrations corresponded to the presence of Gm(b). No clearcut evidence was obtained for a relation between IgG4 concentration and Gm factors, although in general Gm(n) positive individuals had higher and Gm (zag) positive individuals lower concentrations of this subclass in their serum. Quantification of IgG subclasses in sera from healthy children of different ages revealed that the amount of IgG2 rises slowly with age, having not yet reached the adult level at the age of 12 years. This also holds for IgG4, although in a lesser degree. No significant differences from the adult level were found for the concentrations of IgG1 and IgG3.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allansmith M., McClellan B. H., Butterworth M., Maloney J. R. The development of immunoglobulin levels in man. J Pediatr. 1968 Feb;72(2):276–290. doi: 10.1016/s0022-3476(68)80324-5. [DOI] [PubMed] [Google Scholar]
  2. Berg T., Johansson S. G. Immunoglobulin levels during childhood, with special regard to IgE. Acta Paediatr Scand. 1969 Sep;58(5):513–524. doi: 10.1111/j.1651-2227.1969.tb04753.x. [DOI] [PubMed] [Google Scholar]
  3. Collins-Williams C., Tkachyk S. J., Toft B., Moscarello M. Quantitative immunoglobulin levels (IgG, IgA and IgM) in children. Int Arch Allergy Appl Immunol. 1967;31(1):94–103. doi: 10.1159/000229857. [DOI] [PubMed] [Google Scholar]
  4. Froland S. S., Natvig J. B. Surface-bound immunoglobulin on lymphocytes from normal and immunodeficient humans. Scand J Immunol. 1972;1(1):1–12. doi: 10.1111/j.1365-3083.1972.tb03730.x. [DOI] [PubMed] [Google Scholar]
  5. Fröland S. S., Natvig J. B. Lymphocytes with membrane-bound immunoglobulin (B-lymphocytes) in new-born babies. Clin Exp Immunol. 1972 Aug;11(4):495–505. [PMC free article] [PubMed] [Google Scholar]
  6. Geny B., Rodary C., Griscelli C., Mozziconacci P. Immunoglobulin D (IgD) in childhood. I. Maturation of serum IgD: study of a population of healthy children. Biomedicine. 1974 Jan;20(1):40–45. [PubMed] [Google Scholar]
  7. Kunkel H. G., Joslin F. G., Penn G. M., Natvig J. B. Genetic variants of G4 globulin. A unique relationship to other classes of G globulin. J Exp Med. 1970 Sep 1;132(3):508–520. doi: 10.1084/jem.132.3.508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kunkel H. G., Smith W. K., Joslin F. G., Natvig J. B., Litwin S. D. Genetic marker of the gamma-A2 subgroup of gamma-A immunoglobulins. Nature. 1969 Sep 20;223(5212):1247–1248. doi: 10.1038/2231247a0. [DOI] [PubMed] [Google Scholar]
  9. Litwin S. D., Balaban S. Quantitative method for determining human G allotype antigens (Gm). II. Differences in Gm gene expression for G1 and G3 H chains in sera. J Immunol. 1972 Apr;108(4):991–999. [PubMed] [Google Scholar]
  10. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  11. Morell A., Skvaril F., Hitzig W. H., Barandun S. IgG subclasses: development of the serum concentrations in "normal" infants and children. J Pediatr. 1972 Jun;80(6):960–964. doi: 10.1016/s0022-3476(72)80007-6. [DOI] [PubMed] [Google Scholar]
  12. Morell A., Skvaril F., Steinberg A. G., Van Loghem E., Terry W. D. Correlations between the concentrations of the four sub-classes of IgG and Gm Allotypes in normal human sera. J Immunol. 1972 Jan;108(1):195–206. [PubMed] [Google Scholar]
  13. Rowe D. S., Hug K., Faulk W. P., McCormick J. N., Gerber H. IgD on the surface of peripheral blood lymphocytes of the human newborn. Nat New Biol. 1973 Apr 4;242(118):155–157. doi: 10.1038/newbio242155a0. [DOI] [PubMed] [Google Scholar]
  14. Rowe D. S., Hug K., Forni L., Pernis B. Immunoglobulin D as a lymphocyte receptor. J Exp Med. 1973 Oct 1;138(4):965–972. doi: 10.1084/jem.138.4.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Steinberg A. G., Morell A., Skvaril F., van Loghem E. The effect of Gm(23) on the concentration of IgG2 and IgG4 in normal human serum. J Immunol. 1973 Jun;110(6):1642–1645. [PubMed] [Google Scholar]
  16. Stiehm E. R., Fudenberg H. H. Serum levels of immune globulins in health and disease: a survey. Pediatrics. 1966 May;37(5):715–727. [PubMed] [Google Scholar]
  17. Stoop J. W., Zegers B. J., Sander P. C., Ballieux R. E. Serum immunoglobulin levels in healthy children and adults. Clin Exp Immunol. 1969 Jan;4(1):101–112. [PMC free article] [PubMed] [Google Scholar]
  18. Uffelman J. A., Engelhard W. E., Jolliff C. R. Quantitation of immunoglobulins in normal children. Clin Chim Acta. 1970 Apr;28(1):185–192. doi: 10.1016/0009-8981(70)90178-6. [DOI] [PubMed] [Google Scholar]
  19. Van Boxel J. A., Paul W. E., Terry W. D., Green I. Communications. IgD-bearing human lymphocytes. J Immunol. 1972 Sep;109(3):648–651. [PubMed] [Google Scholar]
  20. Wells J. V., Fudenberg H. H., MacKay I. R. RElation of the human antibody response to flagellin to GM genotype. J Immunol. 1971 Dec;107(6):1505–1511. [PubMed] [Google Scholar]
  21. Yount W. J., Kunkel H. G., Litwin S. D. Studies of the Vi (gamma-2c) subgroup of gamma-globulin. A relationship between concentration and genetic type among normal individuals. J Exp Med. 1967 Jan 1;125(1):177–190. doi: 10.1084/jem.125.1.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. van der Giessen M., Freyee W., Rossouw E., van Loghem E. Qualitative and quantitative studies on IgG2 globulins in individual human sera with an antiserum capable of differentiating between Gm(n+) and Gm(n-) proteins. Clin Exp Immunol. 1973 May;14(1):127–139. [PMC free article] [PubMed] [Google Scholar]
  23. van der Giessen M., de Lange B., van der Lee B. The production of precipitating antiglobulin reagents specific for the subclasses of human IgG. Immunology. 1974 Oct;27(4):655–663. [PMC free article] [PubMed] [Google Scholar]

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