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. 1976 Jan;23(1):61–68.

Morphological, immunological and immunocytochemical identification of lymphocytes extracted from cutaneous infiltrates.

A L Claudy, D Schmitt, J Viac, A Alario, M J Staquet, J Thivolet
PMCID: PMC1538365  PMID: 1083319

Abstract

The identification of mononuclear cells extracted from various skin disorders has been investigated and the relative amount of B and T cells determined according to various assays such as E-rosette test, FITC and peroxidase anti-immunoglobulin conjugates. It has been found that: (a) there were two different patterns of distribution of the cells in the infiltrates; one with a T/B ratio above 3 (closest to a delayed type reaction), the other with a T/B ratio below 2. (b) The morphological appearance of tissue lymphocytes differs from that of circulating lymphocytes by the presence of both membrane and cytoplasmic immunoglobulins.

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Selected References

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  1. Abramson N., Gelfand E. W., Jandl J. H., Rosen F. S. The interaction between human monocytes and red cells. Specificity for IgG subclasses and IgG fragments. J Exp Med. 1970 Dec 1;132(6):1207–1215. doi: 10.1084/jem.132.6.1207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Avrameas S. Immunoenzyme techniques: enzymes as markers for the localization of antigens and antibodies. Int Rev Cytol. 1970;27:349–385. doi: 10.1016/s0074-7696(08)61250-4. [DOI] [PubMed] [Google Scholar]
  3. Bianco C., Patrick R., Nussenzweig V. A population of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. I. Separation and characterization. J Exp Med. 1970 Oct 1;132(4):702–720. doi: 10.1084/jem.132.4.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dukor P., Bianco C., Nussenzweig V. Tissue localization of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. Proc Natl Acad Sci U S A. 1970 Oct;67(2):991–997. doi: 10.1073/pnas.67.2.991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Edelson R. L., Smith R. W., Frank M. M., Green I. Identification of subpopulations of mononuclear cells in cutaneous infiltrates. I. Differentiation between B cells, T cells, and histiocytes. J Invest Dermatol. 1973 Aug;61(2):82–89. doi: 10.1111/1523-1747.ep12675403. [DOI] [PubMed] [Google Scholar]
  6. Fröland S., Natvig J. B., Berdal P. Surface-bound immunoglobulin as a marker of B lymphocytes in man. Nat New Biol. 1971 Dec 22;234(51):251–252. doi: 10.1038/newbio234251a0. [DOI] [PubMed] [Google Scholar]
  7. Huber H., Polley M. J., Linscott W. D., Fudenberg H. H., Müller-Eberhard H. J. Human monocytes: distinct receptor sites for the third component of complement and for immunoglobulin G. Science. 1968 Dec 13;162(3859):1281–1283. doi: 10.1126/science.162.3859.1281. [DOI] [PubMed] [Google Scholar]
  8. Jondal M., Holm G., Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972 Aug 1;136(2):207–215. doi: 10.1084/jem.136.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Reyes F., Lejonc J. L., Gourdin M. F., Mannoni P., Dreyfus B. Démonstration de la présence d'immunoglobulines de membrane sur les villosités des lymphocytes humains. C R Acad Sci Hebd Seances Acad Sci D. 1974 Apr 29;278(18):2373–2375. [PubMed] [Google Scholar]
  10. Ross G. D., Rabellino E. M., Polley M. J., Grey H. M. Combined studies of complement receptor and surface immunoglobulin-bearing cells and sheep erythrocyte rosette-forming cells in normal and leukemic human lymphocytes. J Clin Invest. 1973 Feb;52(2):377–385. doi: 10.1172/JCI107194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Silveira N. P., Mendes N. F., Tolnai M. E. Tissue localization of two populations of human lymphocytes distinguished by membrane receptors. J Immunol. 1972 May;108(5):1456–1460. [PubMed] [Google Scholar]
  12. Wybran J., Carr M. C., Fudenberg H. H. The human rosette-forming cell as a marker of a population of thymus-derived cells. J Clin Invest. 1972 Oct;51(10):2537–2543. doi: 10.1172/JCI107069. [DOI] [PMC free article] [PubMed] [Google Scholar]

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