Abstract
A direct macrophage migration test is described, which proved to be useful for the detection of cellular hypersensitivity in man. We used a modification of the method described by Hughes & Paty (1972). In this method the MLR is abolished by 100 rad gamma-irradiation of the peritoneal exudate cells prior to pooling with human lymphocytes. Experiments with various intensity of irradiation, PPD, muscle antigen and encephalitogenic factor were performed to check this method. In a pilot study lymphocytes of patients with diseases of the central or peripheral nervous system or of muscle were tested. This proved that a hypersensitivity of Ef was present in various diseases of the CNS, while in muscle diseases positive tests were found using muscle antigen.
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Selected References
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- Bartfeld H., Atoynatan T. Activity and properties of macrophage migration inhibitory factor produced by mixed lymphocyte cultures. Nat New Biol. 1971 Apr 21;230(16):246–247. doi: 10.1038/newbio230246a0. [DOI] [PubMed] [Google Scholar]
- DAVID J. R., AL-ASKARI S., LAWRENCE H. S., THOMAS L. DELAYED HYPERSENSITIVITY IN VITRO. I. THE SPECIFICITY OF INHIBITION OF CELL MIGRATION BY ANTIGENS. J Immunol. 1964 Aug;93:264–273. [PubMed] [Google Scholar]
- Deibler G. E., Martenson R. E., Kies M. W. Large scale preparation of myelin basic protein from central nervous tissue of several mammalian species. Prep Biochem. 1972;2(2):139–165. doi: 10.1080/00327487208061467. [DOI] [PubMed] [Google Scholar]
- Hughes D. Macrophage migration inhibition test: a critical examination of the technique using a polythene capillary tubing micromethod. J Immunol Methods. 1972 Aug;1(4):403–424. doi: 10.1016/0022-1759(72)90034-8. [DOI] [PubMed] [Google Scholar]
- Hughes D., Paty D. W. Macrophage migration inhibition test applied to delayed hypersensitivity in man. I. A mixed lymphocyte reaction between human and guinea-pig lymphocytes demonstrated using a sensitive micro-capillary method. Z Immunitatsforsch Exp Klin Immunol. 1972;142(5):478–487. [PubMed] [Google Scholar]
- Rajapakse D. A., Glynn L. E. Macrophage migration inhibition test using guinea-pig macrophages and human lymphocytes. Nature. 1970 May 30;226(5248):857–858. doi: 10.1038/226857a0. [DOI] [PubMed] [Google Scholar]
- Rocklin R. E., Meyers O. L., David J. R. An in vitro assay for cellular hypersensitivity in man. J Immunol. 1970 Jan;104(1):95–102. [PubMed] [Google Scholar]
- Soborg M., Bendixen G. Human lymphocyte migration as a parameter of hypersensitivity. Acta Med Scand. 1967 Feb;181(2):247–256. doi: 10.1111/j.0954-6820.1967.tb07255.x. [DOI] [PubMed] [Google Scholar]
- Thor D. E., Jureziz R. E., Veach S. R., Miller E., Dray S. Cell migration inhibition factor released by antigen from human peripheral lymphocytes. Nature. 1968 Aug 17;219(5155):755–757. doi: 10.1038/219755a0. [DOI] [PubMed] [Google Scholar]
- Visakorpi R. The effect of irradiation on macrophage migration inhibition. Immunology. 1974 Jul;27(1):145–149. [PMC free article] [PubMed] [Google Scholar]