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. 1980 Jun;40(3):620–626.

Leucocyte motility in the newborn: determination of spontaneous movement is essential in the in vitro assessment of neutrophil chemotaxis.

H Repo, A M Jokipii, M Leirisalo, T U Kosunen
PMCID: PMC1538952  PMID: 7418269

Abstract

Polymorphonuclear leucocytes (PMNs) of the newborn show poor movement in vitro toward a chemotactic stimulus, such as zymosan-activated human serum (ZAS). This may result from a defect either in spontaneous movement or in the response of the cells to the stimulus. To identify the defect we studied chemotaxis, chemokinesis and spontaneous movement of cord blood PMNs by agarose assay and by the leading front modification of the Boyden chamber technique. Under agarose, cord PMNs showed much spontaneous movement. This, associated with poor stimulated movement, indicated a defect in the responsiveness of cord PMNs to ZAS. In the membrane filter, cord PMNs migrated spontaneously, and in the presence of ZAS, significantly less than adult cells. The weak spontaneous movement most probably resulted from poor deformability of cord PMNs and contributed to the weak stimulated movement of these cells in the filter. Our results indicate that the impaired chemotaxis was due to a defect in both responsiveness and deformability of cord PMNs rather than to a defect in their intrinsic ability to move.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOYDEN S. The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. J Exp Med. 1962 Mar 1;115:453–466. doi: 10.1084/jem.115.3.453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boyum A. Separation of blood leucocytes, granulocytes and lymphocytes. Tissue Antigens. 1974;4(4):269–274. [PubMed] [Google Scholar]
  3. John T. J., Sieber O. F., Jr Chemotactic migration of neutrophils under agarose. Life Sci. 1976 Jan 15;18(2):177–181. doi: 10.1016/0024-3205(76)90022-9. [DOI] [PubMed] [Google Scholar]
  4. Keller H. U., Wilkinson P. C., Abercrombie M., Becker E. L., Hirsch J. G., Miller M. E., Scottramsey W., Zigmond S. H. A proposal for the definition of terms related to locomotion of leucocytes and other cells. Clin Exp Immunol. 1977 Mar;27(3):377–380. [PMC free article] [PubMed] [Google Scholar]
  5. Keller H. U., Wissler J. H., Hess M. W., Cottier H. Distinct chemokinetic and chemotactic responses in neutrophil granulocytes. Eur J Immunol. 1978 Jan;8(1):1–7. doi: 10.1002/eji.1830080102. [DOI] [PubMed] [Google Scholar]
  6. Miler I., Vondrácek J., Hromádková L. The decreased in vitro chemotactic activity of polymorphonuclear leukocytes of newborns and infants. Folia Microbiol (Praha) 1979;24(3):247–252. doi: 10.1007/BF02926456. [DOI] [PubMed] [Google Scholar]
  7. Miller M. E., Oski F. A., Harris M. B. Lazy-leucocyte syndrome. A new disorder of neutrophil function. Lancet. 1971 Apr 3;1(7701):665–669. doi: 10.1016/s0140-6736(71)92679-1. [DOI] [PubMed] [Google Scholar]
  8. Nelson R. D., Quie P. G., Simmons R. L. Chemotaxis under agarose: a new and simple method for measuring chemotaxis and spontaneous migration of human polymorphonuclear leukocytes and monocytes. J Immunol. 1975 Dec;115(6):1650–1656. [PubMed] [Google Scholar]
  9. Pahwa S. G., Pahwa R., Grimes E., Smithwick E. Cellular and humoral components of monocyte and neutrophil chemotaxis in cord blood. Pediatr Res. 1977 May;11(5):677–680. doi: 10.1203/00006450-197705000-00010. [DOI] [PubMed] [Google Scholar]
  10. Repo H., Kostiala A. A., Kosunen T. U. Use of leucocyte migration under agarose to study spontaneous and directed locomotion of leucocytes. Immunology. 1978 Sep;35(3):539–548. [PMC free article] [PubMed] [Google Scholar]
  11. Repo H. Leukocyte migration agarose test for the assessment of human neutrophil chemotaxis. I. Effects of environmental factors on neutrophil migration under agarose. Scand J Immunol. 1977;6(3):203–209. doi: 10.1111/j.1365-3083.1977.tb00385.x. [DOI] [PubMed] [Google Scholar]
  12. Repo H., Vuopio P., Leirisalo M., Jansson S. E., Kosunen T. U. Impaired neutrophil chemotaxis in Pelger-Huët anomaly. Clin Exp Immunol. 1979 May;36(2):326–333. [PMC free article] [PubMed] [Google Scholar]
  13. Zigmond S. H., Hirsch J. G. Leukocyte locomotion and chemotaxis. New methods for evaluation, and demonstration of a cell-derived chemotactic factor. J Exp Med. 1973 Feb 1;137(2):387–410. doi: 10.1084/jem.137.2.387. [DOI] [PMC free article] [PubMed] [Google Scholar]

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