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. 1977 Jan;27(1):178–182.

Drug-induced immunological unresponsiveness: selective inhibition of T-cell 'helper function' by cyclophosphamide in mice pretreated with phytohaemagglutinin.

G Schwarze
PMCID: PMC1540908  PMID: 321157

Abstract

Studies involving the combined use of phytohaemagglutinin (PHA) and cyclophosphamide (CY) indicate that both agents can act together to produce immunological unresponsiveness: following injection of PHA into mice, splenic DNA synthetic responses [14C]thymidine incorporation) and haemolysin plaque formation against sheep red blood cells were determined in daily intervals. Both immunosuppression and DNA synthetic activity were maximally developed 5 days after treatment with PHA. Administration of CY at this time resulted in immunological unresponsiveness lasting for about 18 days. Antibody production could be completely restored with antigen-activated T cells (but not with B cells), thus indicating a selective inhibition of T-cell 'helper function' in mice treated with PHA and CY. This observation is consistent with the general assumption that cells involved in the response to PHA are predominantly T cells. Apparently, these cells are highly sensitive to an inactivation by CY after stimulation with PHA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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