Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1977 Mar;27(3):469–477.

A radiomicroassay for cytotoxic antibody to human spermatozoa. Quantification by tritiated actinomycin d.

J S Sung, H Shizuya, D D Black, D M Mumford
PMCID: PMC1540921  PMID: 862233

Abstract

A radiomicroassay for titration of spermocytotoxic antibody is described. The assay used [3H]AACTINOMYCIN D ([3H]Act D) to label damaged spermatozoa in a fashion analogous to penetration by vital dye. Optimal conditions for and some kinetics of the assay are presented. The assay is sensitive, reliable, simple to perform and uses only small amounts of serum and spermatozoa. Applied to sperm antibody positive human postvasectomy sera, the assay compared favourably in sensitivity eith vital dye microscopic observations and with parallel titration by the Isojima's immobilization tests.

Full text

PDF
469

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brannen G. E., Coffey D. S. Immunologic implications of vasectomy. II. Serum-mediated immunity. Fertil Steril. 1974 Jun;25(6):515–520. doi: 10.1016/s0015-0282(16)40456-5. [DOI] [PubMed] [Google Scholar]
  2. DELAAT A. N. THE COMPLEMENT FIXATION TEST IN VIRUS ANTIBODY STUDIES. Can J Med Technol. 1964 Apr;26:35–41. [PubMed] [Google Scholar]
  3. Fjällbrant B. Interrelation between high levels of sperm antibodies, reduced penetration of cervical mucus by spermatozoa, and sterility in men. Acta Obstet Gynecol Scand. 1968;47(1):102–118. doi: 10.3109/00016346809157471. [DOI] [PubMed] [Google Scholar]
  4. Harrison M. R., Thurman G. B., Thomas G. M. A simple and versatile harvesting device for processing radioactive label incorporated into and-or released from cells in microculture. J Immunol Methods. 1974 Jan;3(1):11–16. doi: 10.1016/0022-1759(74)90027-1. [DOI] [PubMed] [Google Scholar]
  5. Husted S., Hjort T. Microtechnique for simultaneous determination of immobilizing and cytotoxic sperm antibodies. Methodological and clinical studies. Clin Exp Immunol. 1975 Nov;22(2):256–264. [PMC free article] [PubMed] [Google Scholar]
  6. Li T. S. Sperm immunology, infertility, and fertility control. Obstet Gynecol. 1974 Oct;44(4):607–623. [PubMed] [Google Scholar]
  7. Noyes R. W. Antibody binding of spermatozoa. Fertil Steril. 1969 Jan-Feb;20(1):43–49. doi: 10.1016/s0015-0282(16)36903-5. [DOI] [PubMed] [Google Scholar]
  9. Russo J., Metz C. B. The ultrastructural lesions induced by antibody and complement in rabbit spermatozoa. Biol Reprod. 1974 Apr;10(3):293–308. doi: 10.1095/biolreprod10.3.293. [DOI] [PubMed] [Google Scholar]
  10. Sobell H. M., Jain S. C. Stereochemistry of actinomycin binding to DNA. II. Detailed molecular model of actinomycin-DNA complex and its implications. J Mol Biol. 1972 Jul 14;68(1):21–34. doi: 10.1016/0022-2836(72)90259-8. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES