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. 1977 Mar;27(3):407–415.

Immunosuppression by sex steroid hormones. The effect upon PHA- and PPD-stimulated lymphocytes.

F A Wyle, J R Kent
PMCID: PMC1540928  PMID: 862230

Abstract

Progesterone, estradiol, testosterone, cortisol, and 11-desoxycortisol (compound S) were added to cultures of human lymphocytes stimulated with phytohaemagglutinin (PHA) and purified protein derivative (PPD). The immunosuppressive effect of cortisol was verified and the three sex-steroid hormones also were found to inhibit lymphocyte transformation although at concentrations higher than for cortisol. Compound S, a steroid of low biological potency, also had immunosuppressive activity. At concentrations (0-01-1-0 microng/ml), progesterone, oestrogen, testosterone, and Compound S augmented the transformation response to PPD but not to PHA. Marked variation from individual to individual in the suppressive effects of all the steroids were noted. The clinical implications of immunosuppression by the sex steroid hormones are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ablin R. J., Bruns G. R., Guinan P., Bush I. M. The effect of estrogen on the incorporation of 3H-thymidine by PHA-stimulated human peripheral blood lymphocytes. J Immunol. 1974 Aug;113(2):705–707. [PubMed] [Google Scholar]
  2. Ameriks J. A., Thompson N. W., Frey C. F., Appelman H. D., Walter J. F. Hepatic cell adenomas, spontaneous liver rupture, and oral contraceptives. Arch Surg. 1975 May;110(5):548–557. doi: 10.1001/archsurg.1975.01360110094017. [DOI] [PubMed] [Google Scholar]
  3. Barnes E. W., MacCuish A. C., Loudon N. B., Jordan J., Irvine W. J. Phytohaemagglutinin-induced lymphocyte transformation and circulating autoantibodies in women taking oral contraceptives. Lancet. 1974 May 11;1(7863):898–900. doi: 10.1016/s0140-6736(74)90348-1. [DOI] [PubMed] [Google Scholar]
  4. Claman H. N. Corticosteroids and lymphoid cells. N Engl J Med. 1972 Aug 24;287(8):388–397. doi: 10.1056/NEJM197208242870806. [DOI] [PubMed] [Google Scholar]
  5. Cohen J. J. The effects of hydrocortisone on the immune response. Ann Allergy. 1971 Jul;29(7):358–361. [PubMed] [Google Scholar]
  6. Epifanova O. I. Mitotic cycles in estrogen-treated mice: a radioautographic study. Exp Cell Res. 1966 Jun;42(3):562–577. doi: 10.1016/0014-4827(66)90269-2. [DOI] [PubMed] [Google Scholar]
  7. Fitzgerald M. G. The establishment of a normal human population dose-response curve for lymphocytes cultured with PHA (phytohaemagglutinin). Clin Exp Immunol. 1971 Mar;8(3):421–425. [PMC free article] [PubMed] [Google Scholar]
  8. Fudenberg H., Good R. A., Goodman H. C., Hitzig W., Kunkel H. G., Roitt I. M., Rosen F. S., Rowe D. S., Seligmann M., Soothill J. R. Primary immunodeficiencies. Report of a World Health Organization Committee. Pediatrics. 1971 May;47(5):927–946. [PubMed] [Google Scholar]
  9. Heilman D. H., Gambrill M. R., Leichner J. P. The effect of hydrocortisone on the incorporation of tritiated thymidine by human blood lymphocytes cultured with phytohaemagglutinin and pokeweed mitogen. Clin Exp Immunol. 1973 Oct;15(2):203–212. [PMC free article] [PubMed] [Google Scholar]
  10. Hosking C. S., Fitzgerald M. G., Simons M. J. Quantified deficiency of lymphocyte response to phytohaemagglutinin in immune deficiency diseases. Clin Exp Immunol. 1971 Oct;9(4):467–476. [PMC free article] [PubMed] [Google Scholar]
  11. Hulka J. F., Mohr K., Lieberman M. W. Effect of synthetic progestational agents on allograft rejection and circulating antibody production. Endocrinology. 1965 Nov;77(5):897–901. doi: 10.1210/endo-77-5-897. [DOI] [PubMed] [Google Scholar]
  12. KROHN P. L. The effect of ACTH on the reaction to skin homografts in rabbits. J Endocrinol. 1954 Jul;11(1):71–77. doi: 10.1677/joe.0.0110071. [DOI] [PubMed] [Google Scholar]
  13. Kenny J. F., Pangburn P. C., Trail G. Effect of estradiol on immune competence: in vivo and in vitro studies. Infect Immun. 1976 Feb;13(2):448–456. doi: 10.1128/iai.13.2.448-456.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kissling M., Speck B., Goselink H. Effects of hydrocortisone on lymphocytes stimulated by phytohaemagglutinin and pokeweed mitogen. Vox Sang. 1972;23(4):344–349. doi: 10.1111/j.1423-0410.1972.tb03469.x. [DOI] [PubMed] [Google Scholar]
  15. Leibowitz S., Schwartz R. S. Malignancy as a complication of immunosuppressive therapy. Adv Intern Med. 1971;17:95–123. [PubMed] [Google Scholar]
  16. MEDAWAR P. B., SPARROW E. M. The effects of adrenocortical hormones, adrenocorticotrophic hormone and pregnancy on skin transplantation immunity in mice. J Endocrinol. 1956 Nov;14(3):240–256. doi: 10.1677/joe.0.0140240. [DOI] [PubMed] [Google Scholar]
  17. NOWELL P. C. Inhibition of human leukocyte mitosis by prednisolone in vitro. Cancer Res. 1961 Dec;21:1518–1521. [PubMed] [Google Scholar]
  18. Oppenheim J. J., Blaese R. M., Waldmann T. A. Defective lymphocyte transformation and delayed hypersensitivity in Wiskott-Aldrich syndrome. J Immunol. 1970 Apr;104(4):835–844. [PubMed] [Google Scholar]
  19. PELNER L., RHOADES M. G. HOST-TUMOR ANTAGONISM. 34. THE USE OF PROGESTATIONAL AGENTS TO RETARD HOMOGRAFT REJECTION IN RABBITS; A PILOT STUDY. J Am Geriatr Soc. 1965 Aug;13:765–771. doi: 10.1111/j.1532-5415.1965.tb00634.x. [DOI] [PubMed] [Google Scholar]
  20. Rifkind D., Frey J. A., Davis J. R. Influence of gonadal factors on skin test reactivity of CFW mice to Candida albicans. Infect Immun. 1973 Mar;7(3):322–328. doi: 10.1128/iai.7.3.322-328.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Smith D. C., Prentice R., Thompson D. J., Herrmann W. L. Association of exogenous estrogen and endometrial carcinoma. N Engl J Med. 1975 Dec 4;293(23):1164–1167. doi: 10.1056/NEJM197512042932302. [DOI] [PubMed] [Google Scholar]
  22. Stockman G. D., Mumford D. M., Wilbur J. R. A micro-mixed leukocyte culture technique using whole blood. Int Arch Allergy Appl Immunol. 1972;43(5):759–765. doi: 10.1159/000230891. [DOI] [PubMed] [Google Scholar]
  23. Tormey D. C., Fudenberg H. H., Kamin R. M. Effect of prednisolone on synthesis of DNA and RNA by human lymphocytes in vitro. Nature. 1967 Jan 21;213(5073):281–282. doi: 10.1038/213281a0. [DOI] [PubMed] [Google Scholar]
  24. Turcotte J. G., Haines R. F., Brody G. L., Meyer T. J., Schwartz S. A. Immunosuppression with medroxyprogesterone acetate. Transplantation. 1968 Mar;6(2):248–260. doi: 10.1097/00007890-196803000-00010. [DOI] [PubMed] [Google Scholar]
  25. Waltman S. R., Burde R. M., Berrios J. Prevention of corneal homograft rejection by estrogens. Transplantation. 1971 Feb;11(2):194–196. doi: 10.1097/00007890-197102000-00016. [DOI] [PubMed] [Google Scholar]

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