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. 1977 Mar;27(3):531–537.

Autoantibodies against the inner aspect of erythrocyte membranes in NZB mice.

E Linder
PMCID: PMC1540936  PMID: 862240

Abstract

Erythrocyte autoantibodies in NZB mice react by hemagglutination methods with exposed and hidden red cell antigens. The hidden antigens can be exposed by treatment with proteolytic enzymes. By indirect immunofluorescence one antibody population can be shown to react with modified red cells. In the present study the location of the corresponding autoantigen within the membrane was studied. Mechanical or hypotonic lysis of the red cells exposed the antigen. Proteolytic digestion known to expose other erythrocyte autoantigens had no effect. The autoantigen was exposed on 'inside out' erythrocyte membrane vesicles, but not on 'right-side out' vesicles, prepared from isolated erythrocyte ghosts. Frezzing and thawing as well as mechanical disintergration of red cells liberated antigenically active material as saline-insuluble fibrillar material. The observations indicate that the autoantigen studied is located at the inner aspect of the erythrocyte membrane and suggest that it is associated with fibril-forming structural components. The observed reactivity distinguishes the described antibodies from previously identified erythrocyte autoantibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Costea N., Yakulis V., Heller P. Cold reacting antibody in NZB-B1 mice. Blood. 1970 May;35(5):583–590. [PubMed] [Google Scholar]
  2. DODGE J. T., MITCHELL C., HANAHAN D. J. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch Biochem Biophys. 1963 Jan;100:119–130. doi: 10.1016/0003-9861(63)90042-0. [DOI] [PubMed] [Google Scholar]
  3. East J., Prosser P. R. Autoimmunity and malignancy in New Zealand black mice. Proc R Soc Med. 1967 Aug;60(8):823–825. doi: 10.1177/003591576706000855. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Guidotti G. The composition of biological membranes. Arch Intern Med. 1972 Feb;129(2):194–201. [PubMed] [Google Scholar]
  5. Holborow E. J., Barnes R. D., Tuffrey M. A new red-cell autoantibody in NZB mice. Nature. 1965 Aug 7;207(997):601–604. doi: 10.1038/207601a0. [DOI] [PubMed] [Google Scholar]
  6. Howie J. B., Helyer B. J. The immunology and pathology of NZB mice. Adv Immunol. 1968;9:215–266. doi: 10.1016/s0065-2776(08)60444-7. [DOI] [PubMed] [Google Scholar]
  7. LONG G., HOLMES M. C., BURNET F. M. AUTOANTIBODIES PRODUCED AGAINST MOUSE ERYTHROCYTES IN NZB MICE. Aust J Exp Biol Med Sci. 1963 Aug;41:315–322. doi: 10.1038/icb.1963.31. [DOI] [PubMed] [Google Scholar]
  8. Lambert P. H., Dixon F. J. Pathogenesis of the glomerulonephritis of NZB/W mice. J Exp Med. 1968 Mar 1;127(3):507–522. doi: 10.1084/jem.127.3.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Linder E., Edgington T. S. Immunobiology of the autoantibody response. I. Circulating analogues of erythrocyte autoantigens and heterogeneity of the autoimmune response of NZB mice. Clin Exp Immunol. 1973 Feb;13(2):279–292. [PMC free article] [PubMed] [Google Scholar]
  10. Linder E., Edgington T. S. Immunobiology of the autoantibody response. II. The lipoprotein-associated soluble HB erythrocyte autoantigen of NZB mice. J Immunol. 1973 Jan;110(1):53–62. [PubMed] [Google Scholar]
  11. Linder E., Tallberg T. Preparation of anti-IgG conjugates for immunofluorescence using immunoadsorbents for purification. Ann Med Exp Biol Fenn. 1970;48(4):212–220. [PubMed] [Google Scholar]
  12. Marchesi S. L., Steers E., Marchesi V. T., Tillack T. W. Physical and chemical properties of a protein isolated from red cell membranes. Biochemistry. 1970 Jan 6;9(1):50–57. doi: 10.1021/bi00803a007. [DOI] [PubMed] [Google Scholar]
  13. Mellors R. C., Shirai T., Aoki T., Huebner R. J., Krawczynski K. Wild-type Gross leukemia virus and the pathogenesis of the glomerulonephritis of New Zealand mice. J Exp Med. 1971 Jan 1;133(1):113–132. doi: 10.1084/jem.133.1.113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Porter D. D., Porter H. G., Cox N. A. Immune complex glomerulonephritis in one-year-old C57BL-6 mice induced by endogenous murine leukemia virus and erythrocyte antigens. J Immunol. 1973 Dec;111(6):1626–1633. [PubMed] [Google Scholar]
  16. Steck T. L. The organization of proteins in the human red blood cell membrane. A review. J Cell Biol. 1974 Jul;62(1):1–19. doi: 10.1083/jcb.62.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Steck T. L., Weinstein R. S., Straus J. H., Wallach D. F. Inside-out red cell membrane vesicles: preparation and purification. Science. 1970 Apr 10;168(3928):255–257. doi: 10.1126/science.168.3928.255. [DOI] [PubMed] [Google Scholar]
  18. Tilney L. G., Detmers P. Actin in erythrocyte ghosts and its association with spectrin. Evidence for a nonfilamentous form of these two molecules in situ. J Cell Biol. 1975 Sep;66(3):508–520. doi: 10.1083/jcb.66.3.508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yu J., Fischman D. A., Steck T. L. Selective solubilization of proteins and phospholipids from red blood cell membranes by nonionic detergents. J Supramol Struct. 1973;1(3):233–248. doi: 10.1002/jss.400010308. [DOI] [PubMed] [Google Scholar]

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