Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1977 Mar;27(3):446–453.

Human polymorphonuclear leucocytes as mediators of antibody-dependent cellular cytotoxicity to herpes simplex virus-infected cells.

J M Oleske, R B Ashman, S Kohl, S L Shore, S E Starr, P Wood, A J Nahmias
PMCID: PMC1540941  PMID: 193661

Abstract

Human polymorphonuclear leucocytes (PML) were able to mediate antibody-dependent cellular cytotoxicity (ADCC) against target cells acutely infected with type 1 Herpes simplex virus. The reaction mediated by PML occurred more slowly and required higher concentrations of immune serum than that mediated by human mononuclear cells (MC). At the same ratio of effector cells to target cells, PML-mediated ADCC was less than MC-mediated ADCC. The observed relationship between the number of effector cells added, and the number of target cells lysed, showed that cytolysis mediated by both PML and MC was consistent with 'one hit' probability predictions. This suggested that target cell death resulted from an interaction with a single effector cell. The calculated frequency of effector cells in PML preparation was similar to that in MC, approximately 3-5%. Preliminary examination of the nature of the effector cells suggested that they did not comprise a morphologically distinct subclass of PML. These experiments demonstrate a possible new role for PML in host defence against viral infections.

Full text

PDF
446

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Butterworth A. E., Sturrock R. F., Houba V., Mahmoud A. A., Sher A., Rees P. H. Eosinophils as mediators of antibody-dependent damage to schistosomula. Nature. 1975 Aug 28;256(5520):727–729. doi: 10.1038/256727a0. [DOI] [PubMed] [Google Scholar]
  2. Calder E. A., Urbaniak S. J., Penhale W. J., Irvine W. J. Characterization of human lymphoid cell-mediated antibody-dependent cytotoxicity (LDAC). Clin Exp Immunol. 1974 Dec;18(4):579–593. [PMC free article] [PubMed] [Google Scholar]
  3. Gale R. P., Zighelboim J. Modulation of polymorphonuclear leukocyte-mediated antibody-dependent cellular cytotoxicity. J Immunol. 1974 Dec;113(6):1793–1800. [PubMed] [Google Scholar]
  4. Holm G., Hammarström S. Haemolytic activity of human blood monocytes. Lysis of human erythrocytes treated with anti-A serum. Clin Exp Immunol. 1973 Jan;13(1):29–43. [PMC free article] [PubMed] [Google Scholar]
  5. Holm G. Lysis of antibody-treated human erythrocytes by human leukocytes and macrophages in tissue culture. Int Arch Allergy Appl Immunol. 1972;43(5):671–682. doi: 10.1159/000230883. [DOI] [PubMed] [Google Scholar]
  6. Larsson A., Perlmann P. Study of Fab and F(ab') 2 from rabbit IgG for capacity to induce lymphocyte-mediated target cell destruction in vitro. Int Arch Allergy Appl Immunol. 1972;43(1):80–88. doi: 10.1159/000230823. [DOI] [PubMed] [Google Scholar]
  7. Nahmias A. J., Josey W. E., Naib Z. M., Luce C. F., Duffey A. Antibodies to Herpesvirus hominis types 1 and 2 in humans. I. Patients with genital herpetic infections. Am J Epidemiol. 1970 Jun;91(6):539–546. doi: 10.1093/oxfordjournals.aje.a121165. [DOI] [PubMed] [Google Scholar]
  8. Penfold P. L., Greenberg A. H., Roitt I. M. Characteristics of the effector cells mediating cytotoxicity against antibody-coated target cells. III. Ultrastructural studies. Clin Exp Immunol. 1976 Jan;23(1):91–97. [PMC free article] [PubMed] [Google Scholar]
  9. Perlmann P., Perlmann H., Wigzell H. Lymphocyte mediated cytotoxicity in vitro. Induction and inhibition by humoral antibody and nature of effector cells. Transplant Rev. 1972;13:91–114. doi: 10.1111/j.1600-065x.1972.tb00061.x. [DOI] [PubMed] [Google Scholar]
  10. Rager-Zisman B., Bloom B. R. Immunological destruction of herpes simplex virus I infected cells. Nature. 1974 Oct 11;251(5475):542–543. doi: 10.1038/251542a0. [DOI] [PubMed] [Google Scholar]
  11. Ralph P., Prichard J., Cohn M. Reticulum cell sarcoma: an effector cell in antibody-dependent cell-mediated immunity. J Immunol. 1975 Feb;114(2 Pt 2):898–905. [PubMed] [Google Scholar]
  12. Ramshaw I. A. Lysis of herpesvirus-infected cells by immune spleen cells. Infect Immun. 1975 Apr;11(4):767–769. doi: 10.1128/iai.11.4.767-769.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Russell A. S., Percy J. S., Kovithavongs T. Cell-mediated immunity to Herpes simplex in humans: lymphocyte cytotoxicity measured by 51-Cr release from infected cells. Infect Immun. 1975 Feb;11(2):355–359. doi: 10.1128/iai.11.2.355-359.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shore S. L., Black C. M., Melewicz F. M., Wood P. A., Nahmias A. J. Antibody-dependent cell-mediated cytotoxicity to target cells infected with type 1 and type 2 herpes simplex virus. J Immunol. 1976 Jan;116(1):194–201. [PubMed] [Google Scholar]
  15. Shore S. L., Nahmias A. J., Starr S. E., Wood P. A., McFarlin D. E. Detection of cell-dependent cytotoxic antibody to cells infected with herpes simplex virus. Nature. 1974 Sep 27;251(5473):350–352. doi: 10.1038/251350a0. [DOI] [PubMed] [Google Scholar]
  16. Snyderman R., Wohlenberg C., Notkins A. L. Inflammation and viral infection: chemotactic activity resulting from the interaction of antiviral antibody and complement with cells infected with herpes simplex virus. J Infect Dis. 1972 Aug;126(2):207–209. doi: 10.1093/infdis/126.2.207. [DOI] [PubMed] [Google Scholar]
  17. Trinchieri G., Bauman P., De Marchi M., Tökés Z. Antibody-dependent cell-mediated cytotoxicity in humans. I. Characterization of the effector cell. J Immunol. 1975 Jul;115(1):249–255. [PubMed] [Google Scholar]
  18. Walker W. S., Demus A. Antibody-dependent cytolysis of chicken erythrocytes by an in vitro-established line of mouse peritoneal macrophages. J Immunol. 1975 Feb;114(2 Pt 2):765–769. [PubMed] [Google Scholar]
  19. Wisloff F., Froland S. S., Michaelsen T. E. Antibody-dependent cytotoxicity mediated by human Fc-receptor-bearing cells lacking markers for B- and T-lymphocytes. Int Arch Allergy Appl Immunol. 1974;47(1):139–154. doi: 10.1159/000231208. [DOI] [PubMed] [Google Scholar]
  20. Ziegler H. K., Henney C. S. Antibody-dependent cytolytically active human leukocytes: an analysis of inactivation following in vitro interaction with antibody-coated target cells. J Immunol. 1975 Dec;115(6):1500–1504. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES