Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1976 Dec;26(3):449–456.

Evidence for the involvement of IgE-basophil system in acute serum sickness.

J Benveniste, J Egido, V Gutierrez-Millet
PMCID: PMC1540955  PMID: 1009682

Abstract

The role of the basophils in acute serum sickness of rabbits was examined by monitoring daily the absolute number of basophils before, during and after the disease period. After antigen (bovine serum albumin, BSA) elimination, levels of serum IgE and in vitro basophil degranulation in the presence of BSA were determined. The results showed that the onset of glomerular lesions depends upon the simultaneous occurrence of circulating immune complexes greater than 19 S and of an in vivo basophil depletion--probably equivalent to degranulation--reaching 70% of the pre-disease number. Post-disease antigen-dependent in vitro degranulation of the basophils and levels of serum IgE anti BSA did not prove to be good indexes of basophil sensitization. Our data suggest that basophils are instrumental at early stages of the deposition of immune complexes, most probably through their sensitization by membrane-bound IgE antibodies.

Full text

PDF
449

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENACERRAF B., McCLUSKEY R. T., PATRAS D. Localization of colloidal substances in vascular endothelium: a mechanism of tissue damage. I. Factors causing the pathologic deposition of colloidal carbon. Am J Pathol. 1959 Jan-Feb;35(1):75–91. [PMC free article] [PubMed] [Google Scholar]
  2. Barbaro J. F., Zvaifler N. J. Antigen induced histamine release from platelets of rabbits producing homologous PCA antibody. Proc Soc Exp Biol Med. 1966 Aug-Sep;122(4):1245–1247. doi: 10.3181/00379727-122-31371. [DOI] [PubMed] [Google Scholar]
  3. Becker E. L. Nature and classification of immediate-type allergic reactions. Adv Immunol. 1971;13:267–313. doi: 10.1016/s0065-2776(08)60186-8. [DOI] [PubMed] [Google Scholar]
  4. Benveniste J., Henson P. M., Cochrane C. G. Leukocyte-dependent histamine release from rabbit platelets. The role of IgE, basophils, and a platelet-activating factor. J Exp Med. 1972 Dec 1;136(6):1356–1377. doi: 10.1084/jem.136.6.1356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Benveniste J., Le Couedic J. P., Kamoun P. Letter: Aggregation of human platelets by platelet-activating factor. Lancet. 1975 Feb 8;1(7902):344–345. doi: 10.1016/s0140-6736(75)91264-7. [DOI] [PubMed] [Google Scholar]
  6. Benveniste J. Platelet-activating factor, a new mediator of anaphylaxis and immune complex deposition from rabbit and human basophils. Nature. 1974 Jun 7;249(457):581–582. doi: 10.1038/249581a0. [DOI] [PubMed] [Google Scholar]
  7. Bokisch V. A., Müller-Eberhard H. J. Anaphylatoxin inactivator of human plasma: its isolation and characterization as a carboxypeptidase. J Clin Invest. 1970 Dec;49(12):2427–2436. doi: 10.1172/JCI106462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. COCHRANE C. G. STUDIES ON THE LOCALIZATION OF CIRCULATING ANTIGEN-ANTIBODY COMPLEXES AND OTHER MACROMOLECULES IN VESSELS. II. PATHOGENETIC AND PHARMACODYNAMIC STUDIES. J Exp Med. 1963 Oct 1;118:503–513. doi: 10.1084/jem.118.4.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cochrane C. G., Hawkins D. Studies on circulating immune complexes. 3. Factors governing the ability of circulating complexes to localize in blood vessels. J Exp Med. 1968 Jan 1;127(1):137–154. doi: 10.1084/jem.127.1.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GERMUTH F. G., Jr A comparative histologic and immunologic study in rabbits of induced hypersensitivity of the serum sickness type. J Exp Med. 1953 Feb 1;97(2):257–282. doi: 10.1084/jem.97.2.257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Grant J. A., Dupree E., Goldman A. S., Schultz D. R., Jackson A. L. Complement-mediated release of histamine from human leukocytes. J Immunol. 1975 Mar;114(3):1101–1106. [PubMed] [Google Scholar]
  12. Halonen M., Pinckard R. N. Intravascular effects of IgE antibody upon basophils,, neutrophils, platelets and blood coagulation in the rabbit. J Immunol. 1975 Aug;115(2):519–524. [PubMed] [Google Scholar]
  13. Henson P. M., Cochrane C. G. Acute immune complex disease in rabbits. The role of complement and of a leukocyte-dependent release of vasoactive amines from platelets. J Exp Med. 1971 Mar 1;133(3):554–571. doi: 10.1084/jem.133.3.554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Henson P. M., Cochrane C. G. Immunological induction of increased vascular permeability. II. Two mechanisms of histamine release from rabbit platelets involving complement. J Exp Med. 1969 Jan 1;129(1):167–184. doi: 10.1084/jem.129.1.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Henson P. M. Release of vasoactive amines from rabbit platelets induced by sensitized mononuclear leukocytes and antigen. J Exp Med. 1970 Feb;131(2):287–306. doi: 10.1084/jem.131.2.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. KNIKER W. T., COCHRANE C. G. PATHOGENIC FACTORS IN VASULAR LESIONS OF EXPERIMENTAL SERUM SICKNESS. J Exp Med. 1965 Jul 1;122:83–98. doi: 10.1084/jem.122.1.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kniker W. T., Cochrane C. G. The localization of circulating immune complexes in experimental serum sickness. The role of vasoactive amines and hydrodynamic forces. J Exp Med. 1968 Jan 1;127(1):119–136. doi: 10.1084/jem.127.1.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lepow I. H., Willms-Kretschmer K., Patrick R. A., Rosen F. S. Gross and ultrastructural observations on lesions produced by intradermal injection of human C3a in man. Am J Pathol. 1970 Oct;61(1):13–23. [PMC free article] [PubMed] [Google Scholar]
  19. Levine B. B., Vaz N. M. Effect of combinations of inbred strain, antigen, and antigen dose on immune responsiveness and reagin production in the mouse. A potential mouse model for immune aspects of human atopic allergy. Int Arch Allergy Appl Immunol. 1970;39(2-3):156–171. doi: 10.1159/000230343. [DOI] [PubMed] [Google Scholar]
  20. MOTA I. The mechanism of action of anaphylatoxin. Its effect on guinea pig mast cells. Immunology. 1959 Oct;2:403–413. [PMC free article] [PubMed] [Google Scholar]
  21. McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
  22. Okumura K., Tada T. Regulation of homocytotropic antibody formation in the rat. VI. Inhibitory effect of thymocytes on the homocytotropic antibody response. J Immunol. 1971 Dec;107(6):1682–1689. [PubMed] [Google Scholar]
  23. Osler A. G., Lichtenstein L. M., Levy D. A. In vitro studies of human reaginic allergy. Adv Immunol. 1968;8:183–231. doi: 10.1016/s0065-2776(08)60467-8. [DOI] [PubMed] [Google Scholar]
  24. RHYNE M. B., GERMUTH F. G., Jr The relationships between serum complement activity and the development of allergic lesions in rabbits. J Exp Med. 1961 Nov 1;114:633–646. doi: 10.1084/jem.114.5.633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Schoenbechler M. J., Barbaro J. F. The requirement for sensitized lymphocytes in one form of antigen-induced histamine release from rabbit platelets. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1247–1251. doi: 10.1073/pnas.60.4.1247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Siraganian R. P., Osler A. G. Destruction of rabbit platelets in the allergic response of sensitized leukocytes. I. Demonstration of a fluid phase intermediate. J Immunol. 1971 May;106(5):1244–1251. [PubMed] [Google Scholar]
  27. Siraganian R. P., Osler A. G. Destruction of rabbit platelets in the allergic response of sensitized leukocytes. II. Evidence for basophil involvement. J Immunol. 1971 May;106(5):1252–1259. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES