Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1976 Dec;26(3):520–527.

Binding of phytohaemagglutinin to serum substances and inhibition of lymphocyte transformation in Hodgkin's disease.

P L Amlot, A Unger
PMCID: PMC1540991  PMID: 1009684

Abstract

Serum from eleven of fifty-four patients with Hodgkin's disease inhibited phytohaemagglutinin (PHA) stimulation of both autologous and homologous lymphocytes. The inhibitory effect resided in the macromolecular fraction of these sera. PHA was shown to bind to serum substances in inhibitory sera and the degree of PHA binding in the macromolecular fraction correlated directly with the extent of inhibition.

Full text

PDF
520

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BECKMAN L. Effect of phytohaemagglutinin on human serum and cell proteins. Nature. 1962 Aug 11;195:582–583. doi: 10.1038/195582a0. [DOI] [PubMed] [Google Scholar]
  2. Chase P. S. The effects of human serum fractions on phytohemagglutinin- and concanavalin A-stimulated human lymphocyte cultures. Cell Immunol. 1972 Dec;5(4):544–554. doi: 10.1016/0008-8749(72)90104-9. [DOI] [PubMed] [Google Scholar]
  3. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Garrioch D. B., Good R. A., Gatti R. A. Lymphocyte response to P.H.A. in patients with non-lymphoid tumours. Lancet. 1970 Mar 21;1(7647):618–618. doi: 10.1016/s0140-6736(70)91658-2. [DOI] [PubMed] [Google Scholar]
  5. Grifoni V. Recent immunological findins in Hodgkin's disease. Tumori. 1973 Sep-Oct;59(5):363–373. doi: 10.1177/030089167305900508. [DOI] [PubMed] [Google Scholar]
  6. Hersh E. M., Oppenheim J. J. Impaired in vitro lymphocyte transformation in Hodgkin's disease. N Engl J Med. 1965 Nov 4;273(19):1006–1012. doi: 10.1056/NEJM196511042731903. [DOI] [PubMed] [Google Scholar]
  7. Holland N. H., Holland P. Haemagglutinating, precipitating and lymphocyte-stimulating factors of phytohaemagglutinin. Nature. 1965 Sep 18;207(5003):1307–1308. doi: 10.1038/2071307a0. [DOI] [PubMed] [Google Scholar]
  8. Horne C. H., McLay A. L., Tavadia H. B., Carmichael I., Mallinson A. C., Laiwah A. A., Thomas M. A., MacSween R. N. Studies on pregnancy-associated globulin. Clin Exp Immunol. 1973 Apr;13(4):603–611. [PMC free article] [PubMed] [Google Scholar]
  9. Huber H., Pastner D., Dittrich P., Braunsteiner H. In vitro reactivity of human lymphocytes in uraemia--a comparison with the impairment of delayed hypersensitivity. Clin Exp Immunol. 1969 Jul;5(1):75–82. [PMC free article] [PubMed] [Google Scholar]
  10. Levene G. M., Turk J. L., Wright D. J., Grimble A. G. Reduced lymphocyte transformation due to a plasma factor in patients with active syphilis. Lancet. 1969 Aug 2;2(7614):246–247. doi: 10.1016/s0140-6736(69)90010-5. [DOI] [PubMed] [Google Scholar]
  11. MacSween R. N., Thomas M. A. Lymphocyte transformation by phytohaemagglutinin (PHA) and purified protein derivative (PPD) in primary biliary cirrhosis. Evidence of serum inhibitory factors. Clin Exp Immunol. 1973 Dec;15(4):523–533. [PMC free article] [PubMed] [Google Scholar]
  12. Sample W. F., Gertner H. R., Jr, Chretien P. B. Inhibition of phytohemagglutinin-induced in vitro lymphocyte transformation by serum from patients with carcinoma. J Natl Cancer Inst. 1971 Jun;46(6):1291–1297. [PubMed] [Google Scholar]
  13. Suciu-Foca N., Buda J., McManus J., Thiem T., Reemtsma K. Impaired responsiveness of lymphocytes and serum-inhibitory factors in patients with cancer. Cancer Res. 1973 Oct;33(10):2373–2377. [PubMed] [Google Scholar]
  14. Tognella S., Mantovani G., Del Giacco G. S., Manconi P. E., Cengiarotti L., Floris C., Grifoni V. Effetto del siero citotossico di pazienti con malattia di Hodgkin sulla PHA-responsività in vitro di linfociti periferici umani. Tumori. 1975 Jan-Feb;61(1):53–62. doi: 10.1177/030089167506100107. [DOI] [PubMed] [Google Scholar]
  15. Trubowitz S., Masek B., Del Rosario A. Lymphocyte response to phytohemagglutinin in Hodgkin's disease, lymphatic leukemia and lymphosarcoma. Cancer. 1966 Dec;19(12):2019–2023. doi: 10.1002/1097-0142(196612)19:12<2019::aid-cncr2820191228>3.0.co;2-h. [DOI] [PubMed] [Google Scholar]
  16. Whittaker M. G., Rees K., Clark C. G. Reduced lymphocyte transformation in breast cancer. Lancet. 1971 May 1;1(7705):892–893. doi: 10.1016/s0140-6736(71)92448-2. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES