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. 1977 Jun;28(3):394–399.

Histamine-receptor leucocytes (HRL). Organ and lymphoid subpopulation distribution in man.

A Saxon, V D Morledge, B Bonavida
PMCID: PMC1541015  PMID: 330055

Abstract

The frequency of lymphoid cells with a membrane receptor for histamine was determined in various lymphoid organs in man using a histamine-rosette assay. Thymus had very low numbers of histamine-receptor cells while lymph node and peripheral blood had increasing percentages. Through a combination of cell separation techniques, we demonstrated that about one third (1/3) of peripheral blood B lymphocytes and macrophages carry histamine receptors. Immature B cells or null cells (E-rosette and membrane-immunoglobulin-negative) do not have this receptor. Only 10% of peripheral blood T lymphocytes formed histamine rosettes. That these histamine receptor T lymphocytes are a subpopulation representing the differentiated suppressor/cytotoxic T cells is suggested by evidence showing complete removal of histamine receptor T lymphocytes on nylon wool adherence columns. Thus, the histamine receptor is expressed on differentiated B and T lymphocytes and may serve as a marker for developed suppressor/cytotoxic T cells in man.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ballet J. J., Merler E. The separation and reactivity in vitro of a subpopulation of human lymphocytes which bind histamine. Correlation of histamine reactivity with cellular maturation. Cell Immunol. 1976 Jun 15;24(2):250–269. doi: 10.1016/0008-8749(76)90210-0. [DOI] [PubMed] [Google Scholar]
  2. Bourne H. R., Lichtenstein L. M., Melmon K. L., Henney C. S., Weinstein Y., Shearer G. M. Modulation of inflammation and immunity by cyclic AMP. Science. 1974 Apr 5;184(4132):19–28. doi: 10.1126/science.184.4132.19. [DOI] [PubMed] [Google Scholar]
  3. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  4. Cantor H., Boyse E. A. Functional subclasses of T-lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of antigen. J Exp Med. 1975 Jun 1;141(6):1376–1389. doi: 10.1084/jem.141.6.1376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chess L., Levine H., MacDermott R. P., Schlossman S. F. Immunologic functions of isolated human lymphocyte subpopulations. VI. Further characterization of the surface Ig negative, E rosette negative (null cell) subset. J Immunol. 1975 Dec;115(6):1483–1487. [PubMed] [Google Scholar]
  6. Feldmann M., Kontiainen S. Suppressor cell induction in vitro. II. Cellular requirements of suppressor cell induction. Eur J Immunol. 1976 Apr;6(4):302–305. doi: 10.1002/eji.1830060413. [DOI] [PubMed] [Google Scholar]
  7. Greaves M. F., Brown G. Purification of human T and B lymphocytes. J Immunol. 1974 Jan;112(1):420–423. [PubMed] [Google Scholar]
  8. Huber Ch, Wigzell H. A simple rosette assay for demonstration of complement receptor sites using complement-coated zymosan beads. Eur J Immunol. 1976 Jun;5(6):432–435. doi: 10.1002/eji.1830050616. [DOI] [PubMed] [Google Scholar]
  9. Jondal M., Holm G., Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972 Aug 1;136(2):207–215. doi: 10.1084/jem.136.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kedar E., Bonavida B. Histamine receptor-bearing leukocytes (HRL). I. Detection of histamine receptor-bearing cells by rosette formation with histamine-coated erythrocytes. J Immunol. 1974 Nov;113(5):1544–1552. [PubMed] [Google Scholar]
  11. Melmon K. L., Bourne H. R., Weinstein J., Sela M. Receptors for histamine can be detected on the surface of selected leukocytes. Science. 1972 Aug 25;177(4050):707–709. doi: 10.1126/science.177.4050.707. [DOI] [PubMed] [Google Scholar]
  12. Plaut M., Lichtenstein L. M., Henney C. S. Increase in histamine receptors on thymus-derived effector lymphocytes during the primary immune response to alloantigens. Nature. 1973 Aug 3;244(5414):284–287. doi: 10.1038/244284a0. [DOI] [PubMed] [Google Scholar]
  13. Samarut C., Brochier J., Revillard J. P. Distribution of cells binding erythrocyte-antibody (EA) complexes in human lymphoid populations. Scand J Immunol. 1976;5(3):221–231. doi: 10.1111/j.1365-3083.1976.tb00273.x. [DOI] [PubMed] [Google Scholar]
  14. Saxon A., Feldhaus J., Robins R. A. Single step separation of human T and B cells using AET treated srbc rosettes. J Immunol Methods. 1976;12(3-4):285–288. doi: 10.1016/0022-1759(76)90050-8. [DOI] [PubMed] [Google Scholar]
  15. Seeger R. C., Robins R. A., Stevens R. H., Klein R. B., Waldman D. J., Zeltzer P. M., Kessler S. W. Severe combined immunodeficiency with B lymphocytes: in vitro correction of defective immunoglobulin production by addition of normal T lymphocytes. Clin Exp Immunol. 1976 Oct;26(1):1–10. [PMC free article] [PubMed] [Google Scholar]
  16. Shearer G. M., Melmon K. L., Weinstein Y., Sela M. Regulation of antibody response by cells expressing histamine receptors. J Exp Med. 1972 Nov 1;136(5):1302–1307. doi: 10.1084/jem.136.5.1302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Siegal F. P., Siegal M., Good R. A. Suppression of B-cell differentiation by leukocytes from hypogammaglobulinemic patients. J Clin Invest. 1976 Jul;58(1):109–122. doi: 10.1172/JCI108439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Winchester R. J., Fu S. M., Hoffman T., Kunkel H. G. IgG on lymphocyte surfaces; technical problems and the significance of a third cell population. J Immunol. 1975 Apr;114(4):1210–1212. [PubMed] [Google Scholar]
  19. Yam L. T., Li C. Y., Crosby W. H. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971 Mar;55(3):283–290. doi: 10.1093/ajcp/55.3.283. [DOI] [PubMed] [Google Scholar]
  20. Zighelboim J., Gale R. P., Chiu A., Bonavida B., Ossorio R. C., Fahey J. L. Antibody dependent cellular cytotoxicity: cytotoxicity mediated by non t-lymphocytes. Clin Immunol Immunopathol. 1974 Nov;3(2):193–200. doi: 10.1016/0090-1229(74)90005-1. [DOI] [PubMed] [Google Scholar]
  21. Zucker-Franklin D. The percentage of monocytes among "mononuclear" cell fractions obtained from normal human blood. J Immunol. 1974 Jan;112(1):234–240. [PubMed] [Google Scholar]

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