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. 1977 Aug;29(2):229–239.

Stimulation of human lymphocytes in vitro by leucocytes from patients with untreated acute myeloid leukaemia

G M Taylor, S V Jones, J C Ridway, R Harris
PMCID: PMC1541101  PMID: 143355

Abstract

Leucocytes from sixteen untreated acute myeloid leukaemia (AML) patients were tested for their ability to stimulate lymphocytes from each of twelve normal donors. Of 192 tests between the stimulating AML leucocytes, and responding lymphocytes, 42% resulted in positive lymphocyte stimulation, in contrast to one-way mixed lymphocyte culture (MLC) responses, involving the same lymphocyte donors, which were 100% positive. Lack of stimulation by AML leucocytes was significantly associated, in 11% of the tests, with two or more HLA-A and/or -B antigens common to the responding lymphocyte and stimulating AML leucocyte. The most stimulatory of the AML leucocytes were obtained from two high-leucocyte-count acute myelomonocytic leukaemia patients. The stimulatory capacity of AML leucocytes did not correlate with the clinical fate of the cell donor. Presence of contaminating lymphocytes from the patient in the AML leucocyte samples did not account for the differences in stimulatory capacity between AML leucocytes. Limited in vitro viability of AML leucocytes was ruled out as a factor causing poor lymphocyte stimulation. Kinetic studies showed that AML leucocytes induce either an MLC-type response, or no response at all. Differences in response kinetics were observed between two normal and two remission AML patients, the latter receiving active immunotherapy. Pronase treatment of AML leucocytes failed to increase their stimulatory capacity, but distilled H2O markedly reduced it. The significance of the results is discussed in relation to lymphocyte stimulation by other types of allogeneic cell, and in the context of active immunotherapy of AML.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Freeman C. B., Harris R., Geary C. G., Leyland M. J., MaCiver J. E., Delamore I. W. Active immunotherapy used alone for maintenance of patients with acute myeloid leukaemia. Br Med J. 1973 Dec 8;4(5892):571–573. doi: 10.1136/bmj.4.5892.571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Han T., Dadey B. T and B lymphocytes. Exclusive role as responders and stimulators in human one-way mixed lymphocyte reaction. Immunology. 1976 Oct;31(4):643–648. [PMC free article] [PubMed] [Google Scholar]
  3. Han T., Minowada J. A unique 'leukaemic' T lymphoid cell line: absence of stimulating effect in mixed lymphocyte reaction. Lack of MLR-S in leukaemic T lymphoid cells. Clin Exp Immunol. 1973 Dec;15(4):535–541. [PMC free article] [PubMed] [Google Scholar]
  4. Hersey P., MacLennan I. C., Campbell A. C., Harris R., Freeman C. B. Cytotoxicity against human leukaemic cells. I. Demonstration of antibody-dependent lymphocyte killing of human allogeneic myeloblasts. Clin Exp Immunol. 1973 Jun;14(2):159–167. [PMC free article] [PubMed] [Google Scholar]
  5. Klouda T. P., Lawler S. D., Powles R. L., Oliver R. T., Grant C. K. HL-A antibody response in patients with acute myelogenous leukaemia treated by immunotherapy. Transplantation. 1975 Mar;19(3):245–249. doi: 10.1097/00007890-197503000-00008. [DOI] [PubMed] [Google Scholar]
  6. Kuntz M. M., Innes J. B., Weksler M. E. Lymphocyte transformation induced by autologous cells. IV. Human T-lymphocyte proliferation induced by autologous or allogeneic non-T lymphocytes. J Exp Med. 1976 May 1;143(5):1042–1054. doi: 10.1084/jem.143.5.1042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lohrmann H. P., Novikovs L., Graw R. G., Jr Stimulatory capacity of human T and B lymphocytes in the mixed leukocyte culture. Nature. 1974 Jul 12;250(462):144–146. doi: 10.1038/250144a0. [DOI] [PubMed] [Google Scholar]
  8. MacLennan I. C., Gale D. G., Wood J. Resistance of certain leukaemic myeloblasts to immunological attack. Int J Cancer. 1975 Jun 15;15(6):995–999. doi: 10.1002/ijc.2910150616. [DOI] [PubMed] [Google Scholar]
  9. Pegrum G. D., Balfour I. C., Evans C. A., Middleton V. L. Mixed cell cultures of normal and leukaemic cells. J Pathol. 1973 May;110(1):59–66. doi: 10.1002/path.1711100107. [DOI] [PubMed] [Google Scholar]
  10. Potter M. R., Moore M. Human mixed lymphocyte culture using separated lymphocyte populations. Immunology. 1977 Mar;32(3):359–365. [PMC free article] [PubMed] [Google Scholar]
  11. Powles R. L., Crowther D., Bateman C. J., Beard M. E., McElwain T. J., Russell J., Lister T. A., Whitehouse J. M., Wrigley P. F., Pike M. Immunotherapy for acute myelogenous leukaemia. Br J Cancer. 1973 Nov;28(5):365–376. doi: 10.1038/bjc.1973.162. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rühl H., Vogt W., Bochert G., Schmidt S., Moelle R., Schaoua H. Mixed lymphocyte culture stimulatory and responding capacity of lymphocytes from patients with lymphoproliferative diseases. Clin Exp Immunol. 1975 Jan;19(1):55–65. [PMC free article] [PubMed] [Google Scholar]
  13. Taylor G. M., Freeman C. B., Harris R. Response of remission lymphocytes to autochthonous leukaemic myeloblasts. Br J Cancer. 1976 May;33(5):501–511. doi: 10.1038/bjc.1976.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Taylor G. M., Harris R., Freeman C. B. Cell-mediated cytotoxicity as a result of immunotherapy in patients with acute myeloid leukaemia. Br J Cancer. 1976 Feb;33(2):137–143. doi: 10.1038/bjc.1976.18. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Thurman G. B., Strong D. M., Ahmed A., Green S. S., Sell K. W., Hartzman R. J., Bach F. H. Human mixed lymphocyte cultures. Evaluation of a microculture technique utilizing the Multiple Automated Sample Harvester (MASH). Clin Exp Immunol. 1973 Oct;15(2):289–302. [PMC free article] [PubMed] [Google Scholar]
  16. Twomey J. J., Douglass C. C., Morris S. M. Inability of leukocytes to stimulate mixed leukocyte reactions. J Natl Cancer Inst. 1973 Aug;51(2):345–351. [PubMed] [Google Scholar]
  17. Zarling J. M., Raich P. C., McKeough M., Bach F. H. Generation of cytotoxic lymphocytes in vitro against autologous human leukaemia cells. Nature. 1976 Aug 19;262(5570):691–693. doi: 10.1038/262691a0. [DOI] [PubMed] [Google Scholar]

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