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. 1977 Nov;30(2):299–304.

Immunosuppressive effects of lymphocyte (type II) and leucocyte (type I) interferon on primary antibody responses in vivo and in vitro.

J L Virelizier, E L Chan, A C Allison
PMCID: PMC1541117  PMID: 203424

Abstract

We have tested the hypothesis that type II interferon (IF), released by immune lymphocytes after in vivo stimulation with tuberculin, has immunosuppressive effects. Mycobacterium bovis (BCG) infected mice injected with tuberculin showed a very intense suppression of antibody response to sheep erythrocytes. Sera containing lymphocyte IF strongly inhibited primary immune responses to sheep erythrocytes in cultures. Addition of macrophages could not counteract the in vitro immunosuppressive effects of lymphocyte IF, suggesting that the main effect is exerted directly on lymphocytes. Sendai virus-induced leucocyte (type I) IF was also shown to have suppressive effects in vivo and in vitro. However, lymphocyte IF was shown to be much more immunosuppressive than a preparation of type I interferon with equivalent antiviral potency. Thus type II IF, as a product of activated lymphocytes, may have a major immunoregulatory role.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Braun W., Levy H. B. Interferon preparations as modifiers of immune responses. Proc Soc Exp Biol Med. 1972 Dec;141(3):769–773. doi: 10.3181/00379727-141-36868. [DOI] [PubMed] [Google Scholar]
  2. Brodeur B. R., Merigan T. C. Suppressive effect of interferon on the humoral immune response to sheep red blood cells in mice. J Immunol. 1974 Oct;113(4):1319–1325. [PubMed] [Google Scholar]
  3. Chan E. L., Mishell R. I., Mitchell G. F. Cell interaction in an immune response in vitro: requirement for theta-carrying cells. Science. 1970 Dec 11;170(3963):1215–1217. doi: 10.1126/science.170.3963.1215. [DOI] [PubMed] [Google Scholar]
  4. Chester T. J., Paucker K., Merigan T. C. Suppression of mouse antibody producing spleen cells by various interferon preparations. Nature. 1973 Nov 9;246(5428):92–94. doi: 10.1038/246092a0. [DOI] [PubMed] [Google Scholar]
  5. De Maeyer E., De Maeyer-Guignard J., Vandeputte M. Inhibition by interferon of delayed-type hypersensitivity in the mouse. Proc Natl Acad Sci U S A. 1975 May;72(5):1753–1757. doi: 10.1073/pnas.72.5.1753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Egan H. S., Reeder W. J., Ekstedt R. D. Effect of concanavalin A in vivo in suppressing the antibody response in mice. J Immunol. 1974 Jan;112(1):63–69. [PubMed] [Google Scholar]
  7. Gifford G. E., Tibor A., Peavy D. L. Interferon production in mixed lymphocyte cell cultures. Infect Immun. 1971 Jan;3(1):164–166. doi: 10.1128/iai.3.1.164-166.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gisler R. H., Lindahl P., Gresser I. Effects of interferon on antibody synthesis in vitro. J Immunol. 1974 Aug;113(2):438–444. [PubMed] [Google Scholar]
  9. Golub E. S. Brain-associated theta antigen: reactivity of rabbit anti-mouse brain with mouse lymphoid cells. Cell Immunol. 1971 Aug;2(4):353–361. doi: 10.1016/0008-8749(71)90070-0. [DOI] [PubMed] [Google Scholar]
  10. Green J. A., Cooperband S. R., Kibrick S. Immune specific induction of interferon production in cultures of human blood lymphocytes. Science. 1969 Jun 20;164(3886):1415–1417. doi: 10.1126/science.164.3886.1415. [DOI] [PubMed] [Google Scholar]
  11. Gresser I., Tovey M. G., Bandu M. E., Maury C., Brouty-Boyé D. Role of interferon in the pathogenesis of virus diseases in mice as demonstrated by the use of anti-interferon serum. I. Rapid evolution of encephalomyocarditis virus infection. J Exp Med. 1976 Nov 2;144(5):1305–1315. doi: 10.1084/jem.144.5.1305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Heron I., Berg K., Cantell K. Regulatory effect of interferon on T cells in vitro. J Immunol. 1976 Oct;117(4):1370–1373. [PubMed] [Google Scholar]
  13. Johnson H. M., Smith B. G., Baron S. Inhibition of the primary in vitro antibody response by interferon preparations. J Immunol. 1975 Jan;114(1 Pt 2):403–409. [PubMed] [Google Scholar]
  14. Katz D. H., Benacerraf B. The regulatory influence of activated T cells on B cell responses to antigen. Adv Immunol. 1972;15:1–94. doi: 10.1016/s0065-2776(08)60683-5. [DOI] [PubMed] [Google Scholar]
  15. Mobraaten L. E., De Maeyer E., De Maeyer-Guignard J. Prolongation of allograft survival in mice by inducers of interferon. Transplantation. 1973 Nov;16(5):415–420. doi: 10.1097/00007890-197311000-00005. [DOI] [PubMed] [Google Scholar]
  16. Rasmussen L. E., Jordan G. W., Stevens D. A., Merigan T. C. Lymphocyte interferon production and transformation after Herpes simplex infections in humans. J Immunol. 1974 Feb;112(2):728–736. [PubMed] [Google Scholar]
  17. Rich R. R., Pierce C. W. Biological expressions of lymphocyte activation. 3. Suppression of plaque-forming cell responses in vitro by supernatant fluids from concanavalin A-activated spleen cell cultures. J Immunol. 1974 Apr;112(4):1360–1368. [PubMed] [Google Scholar]
  18. Virelizier J. L., Allison A. C., de Maeyer E. Production by mixed lymphocyte cultures of a type II interferon able to protect macrophages against virus infection. Infect Immun. 1977 Aug;17(2):282–285. doi: 10.1128/iai.17.2.282-285.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Virelizier J. L., Virelizier A. M., Allison A. C. The role of circulating interferon in the modifications of immune responsiveness by mouse hepatitis virus (MHV-3). J Immunol. 1976 Sep;117(3):748–753. [PubMed] [Google Scholar]
  20. Wheelock E. F. Interferon-like virus-inhibitor induced in human leukocytes by phytohemagglutinin. Science. 1965 Jul 16;149(3681):310–311. [PubMed] [Google Scholar]
  21. Youngner J. S., Salvin S. B. Production and properties of migration inhibitory factor and interferon in the circulation of mice with delayed hypersensitivity. J Immunol. 1973 Dec;111(6):1914–1922. [PubMed] [Google Scholar]

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