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. 1977 Dec;30(3):379–383.

Multiple myeloma and monoclonal IgA with anti-actin reactivity.

B H Toh, R Ceredig, F N Cornell, F M Clarke
PMCID: PMC1541152  PMID: 342159

Abstract

Serum containing a monoclonal IgA protein from a patient with multiple myeloma gave intense immunofluorescent staining of smooth muscle fibres and the striations of skeletal muscle, cardiac muscle and thymic myoid cells. It also gave a weaker reaction with hepatocytes in a 'polygonal' pattern, and with renal glomeruli in a diffuse pattern. In culture fibroblasts, the serum stained long, parallel cytoplasmic filaments. Specificity of the staining reactions for actin was established by their prevention on serum absorption with skeletal muscle actin, but not by skeletal muscle myosin, tropomyosin or troponin, and by the demonstration that eluates obtained by acid dissociation of the serum-actin precipitates gave the same staining reactions as the original serum. Localization of the anti-actin reactivity to the monoclonal IgA protein was established by the observation that the same staining reactions were obtained with a monospecific IgA conjugate, with the eluate derived from the gamma globulin band of a serumel ectrophoretic strip, and with a purified euglobulin (IgA) fraction; also, the eluates obtained by acid dissociation of the serum actin precipitates contained monoclonal IgA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Biberfeld G., Fagraeus A., Lenkei R. Reaction of human smooth muscle antibody with thyroid cells. Clin Exp Immunol. 1974 Nov;18(3):371–377. [PMC free article] [PubMed] [Google Scholar]
  2. Clarke F. M., Lovell S. J., Masters C. J., Winzor D. J. Beef muscle troponin: evidence for multiple forms of troponin-T. Biochim Biophys Acta. 1976 Apr 14;427(2):617–626. doi: 10.1016/0005-2795(76)90205-1. [DOI] [PubMed] [Google Scholar]
  3. Gabbiani G., Ryan G. B., Lamelin J. P., Vassalli P., Majno G., Bouvier C. A., Cruchaud A., Lüscher E. F. Human smooth muscle autoantibody. Its identification as antiactin antibody and a study of its binding to "nonmuscular" cells. Am J Pathol. 1973 Sep;72(3):473–488. [PMC free article] [PubMed] [Google Scholar]
  4. Holborow E. J. Smooth-muscle autoantibodies, viral infections and malignant disease. Proc R Soc Med. 1972 May;65(5):481–484. doi: 10.1177/003591577206500524. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lidman K., Biberfeld G., Fagraeus A., Norberg R., Torstensson R., Utter G., Carlsson L., Luca J., Lindberg U. Anti-actin specificity of human smooth muscle antibodies in chronic active hepatitis. Clin Exp Immunol. 1976 May;24(2):266–272. [PMC free article] [PubMed] [Google Scholar]
  6. Roux M. E., Florin-Christensen A., Arana R. M., Doniach D. Paraproteins with antibody activity in acute viral hepatitis and chronic autoimmune liver diseases. Gut. 1974 May;15(5):396–400. doi: 10.1136/gut.15.5.396. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Salmon S. E., Seligmann M. B-cell neoplasia in man. Lancet. 1974 Nov 23;2(7891):1230–1233. doi: 10.1016/s0140-6736(74)90748-x. [DOI] [PubMed] [Google Scholar]
  8. Seligmann M., Brouet J. C. Antibody activity of human myeloma globulins. Semin Hematol. 1973 Apr;10(2):163–177. [PubMed] [Google Scholar]
  9. Strauss A. J., van der Geld H. W., Kemp P. G., Jr, Exum E. D., Goodman H. C. Immunological concomitants of myasthenia gravis. Ann N Y Acad Sci. 1965 Jun 30;124(2):744–766. doi: 10.1111/j.1749-6632.1965.tb18999.x. [DOI] [PubMed] [Google Scholar]
  10. Toh B. H., Gallichio H. A., Jeffrey P. L., Livett B. G., Muller H. K., Cauchi M. N., Clarke F. M. Anti-actin stains synapses. Nature. 1976 Dec 16;264(5587):648–650. doi: 10.1038/264648a0. [DOI] [PubMed] [Google Scholar]
  11. Toh B. H., Hard G. C., Cauchi M. N., Muller H. K. Smooth-muscle-associated contractile protein in renal mesenchymal tumour cells and in transformed cells from DMN-injected rats. Br J Cancer. 1976 Nov;34(5):533–545. doi: 10.1038/bjc.1976.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Toh B. H., Muller H. K., Elrick W. L. Smooth muscle associated antigen in astrocytes and astrocytomata. Br J Cancer. 1976 Feb;33(2):195–202. doi: 10.1038/bjc.1976.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wager O., Räsänen J. A., Haltia K., Wasastjerna C. M components with antibody activity. Anti-smooth muscle, anti-thyroglobulin and anti-streptolysin-O activity in five M component sera. Ann Clin Res. 1971 Apr;3(2):86–97. [PubMed] [Google Scholar]
  14. Whittingham S., Mackay I. R., Irwin J. Autoimmune hepatitis. Immunofluorescence reactions with cytoplasm of smooth muscle and renal glomerular cells. Lancet. 1966 Jun 18;1(7451):1333–1335. doi: 10.1016/s0140-6736(66)92131-3. [DOI] [PubMed] [Google Scholar]
  15. Wilson I. D., Williams R. C., Jr Two distinct groups of immunoglobulin A(IgA) revealed by peptic digestion. J Clin Invest. 1969 Dec;48(12):2409–2416. doi: 10.1172/JCI106207. [DOI] [PMC free article] [PubMed] [Google Scholar]

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