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. 1977 Oct;30(1):70–76.

Biosynthesis and characterization of intracellular IgDkappa in a case of CLL.

J Gordon, J L Smith, D Newell, M Chisholm, G Corte, A Warley, N Richardson
PMCID: PMC1541168  PMID: 414863

Abstract

A case of chronic lymphocytic leukaemia (CLL) with diffuse intracellular IgDkappa is reported. No serum paraprotein or urinary Bence-Jones protein were detected. No surface immunoglobulin was found on the neoplastic lymphocytes, but the cells had receptors for Fcgamma and the C3 component of complement consistent with other cases of CLL. Biosynthetic studies confirmed that the cells synthesized IgDkappa but there was no evidence for secretion of IgD into the culture medium. The cells did not produce Ig of any other class. The intracellular IgD occurred predominantly as deltakappa units with no covalent links between the chains. These findings are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cawley J. C., Smith J., Goldstone A. H., Emmines J., Hamblin J., Hough L. IgA and IgM cytoplastic inclusions in a series of cases of chronic lymphocytic leukaemia. Clin Exp Immunol. 1976 Jan;23(1):78–82. [PMC free article] [PubMed] [Google Scholar]
  2. Corte G., Ferrarini M., Tonda P., Bargellesi A. Increased serum IgD concentrations in patients with Hodgkin's disease. Clin Exp Immunol. 1977 May;28(2):359–362. [PMC free article] [PubMed] [Google Scholar]
  3. Ferrarini M., Bargellesi A., Corte G., Viale G., Pernis B. Comparative study of membrane and intracytoplasmic immunoglobulin classes in human lymphoid cells. Ann N Y Acad Sci. 1975 Jun 30;254:243–253. doi: 10.1111/j.1749-6632.1975.tb29174.x. [DOI] [PubMed] [Google Scholar]
  4. Ferrarini M., Viale G., Risso A., Pernis B. A study of immunoglobulin classes present on the membrane and in the cytoplasm of human tonsil plasma cells. Eur J Immunol. 1976 Aug;6(8):562–565. doi: 10.1002/eji.1830060807. [DOI] [PubMed] [Google Scholar]
  5. Fu S. M., Winchester R. J., Kunkel H. G. Occurrence of surface IgM, IgD, and free light chains of human lymphocytes. J Exp Med. 1974 Feb 1;139(2):451–456. doi: 10.1084/jem.139.2.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hurez D., Flandrin G., Preud'homme J. L., Seligmann M. Unreleased intracellular monoclonal macroglobulin in chronic lymphocytic leukaemia. Clin Exp Immunol. 1972 Feb;10(2):223–234. [PMC free article] [PubMed] [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  8. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  9. Maizel J. V., Jr Acrylamide-gel electrophorograms by mechanical fractionation: radioactive adenovirus proteins. Science. 1966 Feb 25;151(3713):988–990. doi: 10.1126/science.151.3713.988. [DOI] [PubMed] [Google Scholar]
  10. Marchalonis J. J., Cone R. E., Santer V. Enzymic iodination. A probe for accessible surface proteins of normal and neoplastic lymphocytes. Biochem J. 1971 Oct;124(5):921–927. doi: 10.1042/bj1240921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Melchers F., Andersson J. Synthesis, surface deposition and secretion of immunoglobulin M in bone marrow-derived lymphocytes before and after mitogenic stimulation. Transplant Rev. 1973;14:76–130. doi: 10.1111/j.1600-065x.1973.tb00103.x. [DOI] [PubMed] [Google Scholar]
  12. Nies K. M., Marshall J., Oberlin M. A., Halpern M. S., Brown J. C. Chronic lymphocytic leukemia with gamma chain cytoplasmic inclusions. Am J Clin Pathol. 1976 Jun;65(6):948–956. doi: 10.1093/ajcp/65.6.948. [DOI] [PubMed] [Google Scholar]
  13. Payne S. V., Jones D. B., Haegert D. G., Smith J. L., Wright D. H. T and B lymphocytes and Reed-Sternberg cells in Hodgkin's disease lymph nodes and spleens. Clin Exp Immunol. 1976 May;24(2):280–286. [PMC free article] [PubMed] [Google Scholar]
  14. Pernis B., Governa M., Rowe D. S. Light chain types in plasma cells that produce IgD. Immunology. 1969 May;16(5):685–689. [PMC free article] [PubMed] [Google Scholar]
  15. Rowe D. S., Hug K., Forni L., Pernis B. Immunoglobulin D as a lymphocyte receptor. J Exp Med. 1973 Oct 1;138(4):965–972. doi: 10.1084/jem.138.4.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rudders R. A. B lymphocyte subpopulations in chronic lymphocytic leukemia. Blood. 1976 Feb;47(2):229–235. [PubMed] [Google Scholar]
  17. Smith J. L., Haegert D. B- and T-lymphocyte markers on transformed lymphocytes from mitogen-stimulated cultures of normal and CLL lymphocytes and on tonsil blasts. Clin Exp Immunol. 1974 Aug;17(4):547–560. [PMC free article] [PubMed] [Google Scholar]

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