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. 1978 Mar;31(3):357–366.

A new pattern of non-organ- and non-species-specific anti-organelle antibody detected by immunofluorescence: the mitochondrial antibody number 5.

M T Labro, M C Andrieu, M Weber, J C Homberg
PMCID: PMC1541239  PMID: 95913

Abstract

About 0.1% of the sera in human pathology produce a peculiar, cytoplasmic, non-organ- and non-species-specific fluorescence. This may easily be differentiated from the already described anti-organelle antibodies and, more particularly, from the mitochondrial antibodies of primary biliary cirrhosis. Should rat tissues be used in the immunofluorescence test, fluorescence predominates over the first two portions of the renal proximal tubules (P1 and P2) and the mucous neck cells of the stomach. This pattern may be atrributed to mitochondria, and in particular to their inner membranes by fluorescent staining of the ellipsoid region of the rods and cones of the eyes, and by absorption with purified organelles. To distinguish this antibody from the already described mitochondrial antibodies, this one will be called mitochondrial antibody number 5 (M5). The seven carriers of this antibody suffer from systemic lupus erythematosus or autoimmune haemolytic anaemia. In these cases no diseases of the liver were observed, contrary to other classical mitochondrial antibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ben-Yoseph Y., Shapira E., Doniach D. Further purification of the mitochondrial inner membrane autoantigen reacting with primary biliary cirrhosis sera. Immunology. 1974 Feb;26(2):311–321. [PMC free article] [PubMed] [Google Scholar]
  2. Berg P. A., Binder T. Demonstration of mitochondrial antibodies in sera from patients with chronic liver diseases reacting with two different types of complement-fixing antigens. Minerva Gastroenterol. 1976 Jul-Sep;22(3):185–189. [PubMed] [Google Scholar]
  3. Berg P. A. Chronisch-aktive (aggressive) Hepatitis. Zur Differentialdiagnose der lupoiden (ANA-positiven) und cholestatisch verlaufenden (AMA-positiven) Hepatitis. Dtsch Med Wochenschr. 1976 Oct 15;101(42):1536–1543. doi: 10.1055/s-0028-1104369. [DOI] [PubMed] [Google Scholar]
  4. Bianchi F. B., Rizzetto M., Penfold P., Swana G. T., Doniach D. Ultrastructural localization and characterization of a ribosomal antibody detected by immunofluorescence in systemic lupus erythematosus. Clin Exp Immunol. 1974 Aug;17(4):629–636. [PMC free article] [PubMed] [Google Scholar]
  5. DeHeer D. H., Pinckard R. N., Olson M. S. Autoantigenic specificities of liver mitochondrial membranes. Clin Exp Immunol. 1972 Jan;10(1):77–87. [PMC free article] [PubMed] [Google Scholar]
  6. Doniach D., Delhanty J., Lindqvist H. J., Catterall R. D. Mitochondrial and other tissue autoantibodies in patients with biological false positive reactions for syphilis. Clin Exp Immunol. 1970 Jun;6(6):871–884. [PMC free article] [PubMed] [Google Scholar]
  7. Doniach D., Lindqvist H. L., Berg P. A. Non-organ-specific cytoplasmic antibodies detected by immunofluorescence. Int Arch Allergy Appl Immunol. 1971;41(4):501–513. doi: 10.1159/000230545. [DOI] [PubMed] [Google Scholar]
  8. Godin C., Kruh J., Dreyfus J. C. Reconstituted reticulocyte ribosomes active in hemoglobin synthesis. Biochim Biophys Acta. 1969 May 20;182(1):175–179. doi: 10.1016/0005-2787(69)90532-2. [DOI] [PubMed] [Google Scholar]
  9. Homberg J. C., Micouin C., Peltier A., Salmon C., Caroli J. Un nouvel anticorps non spécifique d'organe au cours d'hépatite chronique. Med Chir Dig. 1974;3(2):85–92. [PubMed] [Google Scholar]
  10. Homberg J. C., Rizzetto M., Doniach D. Ribosomal antibodies detected by immunofluorescence in systemic lupus erythematosus and other collagenoses. Clin Exp Immunol. 1974 Aug;17(4):617–628. [PMC free article] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Maas D., Schubothe H. Ein lupus-erythematodes-ähnliches Syndrom mit antimitochondrialen Antikörpern. Dtsch Med Wochenschr. 1973 Jan 26;98(4):131–139. doi: 10.1055/s-0028-1106763. [DOI] [PubMed] [Google Scholar]
  13. Maas D., Schubothe H., Sennekamp J., Genth E., Maerker-Alzer G., Droese M., Hartl P. W., Schumacher K. Zur Frage einer Induzierbarkeit des Pseudo-LE-Syndroms durch Arzneimittel. Vorläufige Ergebnisse von Erhebungen bei 58 Fällen. Dtsch Med Wochenschr. 1975 Jul 25;100(30):1555–1557. doi: 10.1055/s-0028-1106420. [DOI] [PubMed] [Google Scholar]
  14. Parsons D. F., Williams G. R., Chance B. Characteristics of isolated and purified preparations of the outer and inner membranes of mitochondria. Ann N Y Acad Sci. 1966 Jul 14;137(2):643–666. doi: 10.1111/j.1749-6632.1966.tb50188.x. [DOI] [PubMed] [Google Scholar]
  15. Rizzetto M., Bianchi F. B., Doniach D. Characterization of the microsomal antigen related to a subclass of active chronic hepatitis. Immunology. 1974 Mar;26(3):589–601. [PMC free article] [PubMed] [Google Scholar]
  16. Rizzetto M., Swana G., Doniach D. Microsomal antibodies in active chronic hepatitis and other disorders. Clin Exp Immunol. 1973 Nov;15(3):331–344. [PMC free article] [PubMed] [Google Scholar]
  17. STERNBERG W. H., FARBER E., DUNLAP C. E. Histochemical localization of specific oxidative enzymes. II. Localization of diphosphopyridine nucleotide and triphosphopyridine nucleotide diaphorases and the succindehydrogenase system in the kidney. J Histochem Cytochem. 1956 May;4(3):266–283. doi: 10.1177/4.3.266. [DOI] [PubMed] [Google Scholar]
  18. WALKER J. G., DONIACH D., ROITT I. M., SHERLOCK S. SEROLOGICAL TESTS IN DIAGNOSIS OF PRIMARY BILIARY CIRRHOSIS. Lancet. 1965 Apr 17;1(7390):827–831. doi: 10.1016/s0140-6736(65)91372-3. [DOI] [PubMed] [Google Scholar]
  19. WELLER T. H., COONS A. H. Fluorescent antibody studies with agents of varicella and herpes zoster propagated in vitro. Proc Soc Exp Biol Med. 1954 Aug-Sep;86(4):789–794. doi: 10.3181/00379727-86-21235. [DOI] [PubMed] [Google Scholar]
  20. Wright D. J., Doniach D., Lessof M. H., Turk J. L., Grimble A. S., Catterall R. D. New antibody in early syphilis. Lancet. 1970 Apr 11;1(7650):740–743. doi: 10.1016/s0140-6736(70)90972-4. [DOI] [PubMed] [Google Scholar]

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