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. 1978 Mar;31(3):426–435.

Modification of lymphocyte responsiveness in vitro by lambda carrageenan compared with colloidal silica and depletion of surface adherent cells.

G Pawelec, G Brons
PMCID: PMC1541245  PMID: 207475

Abstract

The effects of the immunosuppressive sulphated polygalactan lambda carrageenan on in vitro models of allograft immunity were compared with the effects of removing macrophages (surface adherent and/or phagocytic cells) by established methods. Carrageenan depressed the primary mixed lymphocyte reactions, but not to the same extent as the removal of macrophages. 2-Mercaptoethanol restored the response. Secondary mixed lymphocyte reactions and responses to phytohaemaglutinin were depressed by carrageenan but not by the removal of macrophages, and in these systems 2-mercaptoethanol failed to restore the responses of carrageenan-treated cultures. In contrast, cell-mediated cytolysis by presensitized lymphocytes was not affected by carrageenan or by colloidal silica. Carrageenan depressed cell-mediated cytolysis only if it was present during the sensitization of the effector cells. We conclude that carrageenan can have two dose-related effects in vitro: one on the macrophage and one on the responding lymphocyte.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison A. C., Harington J. S., Birbeck M. An examination of the cytotoxic effects of silica on macrophages. J Exp Med. 1966 Aug 1;124(2):141–154. doi: 10.1084/jem.124.2.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Alter B. J., Bach F. H. Lymphocyte reactivity in vitro. I. Cellular reconstitution of purified lymphocyte response. Cell Immunol. 1970 Jul;1(2):207–218. doi: 10.1016/0008-8749(70)90008-0. [DOI] [PubMed] [Google Scholar]
  3. Argyris B. The role of macrophages, thymus- and bone marrow-derived cells in the graft-versus-host reaction. Transplantation. 1974 Apr;17(4):387–391. doi: 10.1097/00007890-197404000-00008. [DOI] [PubMed] [Google Scholar]
  4. Aschheim L., Raffel S. The immunodepressant effect of carrageenin. J Reticuloendothel Soc. 1972 Mar;11(3):253–262. [PubMed] [Google Scholar]
  5. BORSOS T., RAPP H. J., CRISLER C. THE INTERACTION BETWEEN CARRAGEENAN AND THE FIRST COMPONENT OF COMPLEMENT. J Immunol. 1965 May;94:662–666. [PubMed] [Google Scholar]
  6. Bash J. A., Singer A. M., Waksman B. H. The suppressive effect of immunization on the proliferative responses of rat T cells in vitro. II. Abrogation of antigen-induced suppression by selective cytotoxic agents. J Immunol. 1976 May;116(5):1350–1353. [PubMed] [Google Scholar]
  7. Berke G., Sullivan K. A., Amos B. Rejection of ascites tumor allografts. I. Isolation, characterization, and in vitro reactivity of peritoneal lymphoid effector cells from BALB-c mice immune to EL4 leukosis. J Exp Med. 1972 Jun 1;135(6):1334–1350. doi: 10.1084/jem.135.6.1334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Bevan M. J., Epstein R., Cohn M. The effect of 2-mercaptoethanol on murine mixed lymphocyte cultures. J Exp Med. 1974 Apr 1;139(4):1025–1030. doi: 10.1084/jem.139.4.1025. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Bice D., Schwartz H. J., Lake W. W., Salvaggio J. The effect of carrageenan on the establishment of delayed hypersensitivity. Int Arch Allergy Appl Immunol. 1971;41(4):628–636. doi: 10.1159/000230555. [DOI] [PubMed] [Google Scholar]
  10. Blitstein-Willinger E., Schulz G., Diamantstein T. Changes in thymocyte reactivity to lectins induced by B-cell mitogens of the type of sulphated polyanions. Immunology. 1976 Apr;30(4):529–535. [PMC free article] [PubMed] [Google Scholar]
  11. Bradley B. A., Edwards J. M., Franks D. Histocompatibility phenotyping by the mixed lymphocyte reaction. Tissue Antigens. 1973;3(4):340–347. doi: 10.1111/j.1399-0039.1973.tb01011.x. [DOI] [PubMed] [Google Scholar]
  12. Chen C., Hirsch J. G. The effects of mercaptoethanol and of peritoneal macrophages on the antibody-forming capacity of nonadherent mouse spleen cells in vitro. J Exp Med. 1972 Sep 1;136(3):604–617. doi: 10.1084/jem.136.3.604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Chused T. M., Kassan S. S., Mosier D. E. Macrophage requirement for the in vitro response to TNP Ficoll: a thymic independent antigen. J Immunol. 1976 Jun;116(6):1579–1581. [PubMed] [Google Scholar]
  14. Daniel M. R., Edwards M. J. Interaction of pig lymphocytes with allogenic kidney cells in vitro. Br J Exp Pathol. 1975 Aug;56(4):349–357. [PMC free article] [PubMed] [Google Scholar]
  15. DeBono D. P., Macintyre D. E., White D. J., Gordon J. L. Endothelial adenine uptake as an assay for cell- or complement-mediated cytotoxicity. Immunology. 1977 Feb;32(2):221–226. [PMC free article] [PubMed] [Google Scholar]
  16. Franks D., Bradley B. A. Statistics in cellular typing. Tissue Antigens. 1977 Jul;10(1):1–15. doi: 10.1111/j.1399-0039.1977.tb00746.x. [DOI] [PubMed] [Google Scholar]
  17. Golstein P., Blomgren H. Further evidence for autonomy of T cells mediating specific in vitro cytotoxicity: efficiency of very small amounts of highly purified T cells. Cell Immunol. 1973 Oct;9(1):127–141. doi: 10.1016/0008-8749(73)90174-3. [DOI] [PubMed] [Google Scholar]
  18. Hanna N., Leskowitz S. Structural requirements for in vivo and in vitro immunogenicity in hapten-specific delayed hypersensitivity. Cell Immunol. 1973 May;7(2):189–197. doi: 10.1016/0008-8749(73)90241-4. [DOI] [PubMed] [Google Scholar]
  19. Lemke H., Opitz H. G. Function of 2-mercaptoethanol as a macrophage substitute in the primary immune response in vitro. J Immunol. 1976 Aug;117(2):388–395. [PubMed] [Google Scholar]
  20. Lotzová E., Cudkowicz G. Abrogation of resistance to bone marrow grafts by silica particles. Prevention of the silica effect by the macrophage stabilizer poly-2-vinylpyridine N-oxide. J Immunol. 1974 Sep;113(3):798–803. [PubMed] [Google Scholar]
  21. Pawelec G., Bradley B. A., Puntis M. C. Is there a significant gene dose effect in the primed lymphocyte test? Tissue Antigens. 1977 Nov;10(5):353–360. doi: 10.1111/j.1399-0039.1977.tb00770.x. [DOI] [PubMed] [Google Scholar]
  22. Rios A., Simmons R. L. Poly-2-vinylpyridine N-oxide reverses the immunosuppressive effects of silica and carrageenan. Transplantation. 1972 Mar;13(3):343–345. doi: 10.1097/00007890-197203000-00026. [DOI] [PubMed] [Google Scholar]
  23. Sawicki J. E., Catanzaro P. J. Selective macrophage cytotoxicity of carrageenan in vivo. Int Arch Allergy Appl Immunol. 1975;49(5):709–714. doi: 10.1159/000231451. [DOI] [PubMed] [Google Scholar]
  24. Schwartz H. J., Catanzaro P. J. The differential suppression of antigen, lymphokine and mitogen-induced delayed hypersensitivity-type skin reactions by carrageenan. Int Arch Allergy Appl Immunol. 1973;44(3):409–421. doi: 10.1159/000230948. [DOI] [PubMed] [Google Scholar]
  25. Schwartz H. J., Kellermeyer R. W. Carrageenan and delayed hypersensitivity. II. Activation of Hageman factor by carrageenan and its possible significance. Proc Soc Exp Biol Med. 1969 Dec;132(3):1021–1024. doi: 10.3181/00379727-132-34358. [DOI] [PubMed] [Google Scholar]
  26. Schwartz H. J., Leskowitz S. The effect of carrageenan on delayed hypersensitivity reactions. J Immunol. 1969 Jul;103(1):87–91. [PubMed] [Google Scholar]
  27. Sondel P. M., Chess L., MacDermott R. P., Schlossman S. F. Immunologic functions of isolated human lymphocyte subpopulations. III. Specific allogeneic lympholysis mediated by human T cells alone. J Immunol. 1975 Mar;114(3):982–987. [PubMed] [Google Scholar]
  28. Thomson A. W., Horne C. H. Failure of carrageenan to affect graft-versus-host reactivity in the rat. Transplantation. 1975 Nov;20(5):435–437. [PubMed] [Google Scholar]
  29. Twomey J. J., Sharkey O., Jr, Brown J. A., Laughter A. H., Jordan P. H., Jr Cellular requirements for the mitotic response in allogeneic mixed leukocyte cultures. J Immunol. 1970 Apr;104(4):845–853. [PubMed] [Google Scholar]
  30. Wagner H., Feldmann M., Boyle W., Schrader J. W. Cell-mediated immune response in vitro. 3. The requirement for macrophages in cytotoxic reactions against cell-bound and subcellular alloantigens. J Exp Med. 1972 Aug 1;136(2):331–343. doi: 10.1084/jem.136.2.331. [DOI] [PMC free article] [PubMed] [Google Scholar]

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