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. 1978 May;32(2):361–365.

A new approach to the autoradiographic study of proliferating lymphocytes.

B Zweiman, R P Lisak
PMCID: PMC1541260  PMID: 668206

Abstract

An adaptation of a cell filtration method for the autoradiographic study of cultured lymphocytes has been developed. The percentages of labelled cells are very similar in the filtered cell population to those obtained from replicate cultures processed by centrifugation. The filter method permitted a higher recovery of cells from small numbers in culture with better cytological detail than seen when cells were washed by repeated centrifugation, then suspended and smeared.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baringer J. R. A simplified procedure for spinal fluid cytology. Arch Neurol. 1970 Apr;22(4):305–308. doi: 10.1001/archneur.1970.00480220019004. [DOI] [PubMed] [Google Scholar]
  2. Levinson A. I., Lisak R. P., Zweiman B. A microtechnique for PHA transformation of 5000 separated lymphoyctes. Cell Immunol. 1974 Nov;14(2):321–326. doi: 10.1016/0008-8749(74)90216-0. [DOI] [PubMed] [Google Scholar]
  3. Levinson A. I., Lisak R. P., Zweiman B. Immunologic characterization of cerebrospinal fluid lymphocytes: preliminary report. Neurology. 1976 Jul;26(7):693–695. doi: 10.1212/wnl.26.7.693. [DOI] [PubMed] [Google Scholar]
  4. Lisak R. P., Zweiman B. Immune responses to myelin basic protein in mycobacterial-induced suppression of experimental allergic encephalomyelitis. Cell Immunol. 1974 Nov;14(2):242–254. doi: 10.1016/0008-8749(74)90209-3. [DOI] [PubMed] [Google Scholar]
  5. Sandberg-Wollheim M. Immunoglobulin synthesis in vitro by cerebrospinal fluid cells in patients with multiple sclerosis. Scand J Immunol. 1974;3(6):717–730. doi: 10.1111/j.1365-3083.1974.tb01307.x. [DOI] [PubMed] [Google Scholar]
  6. Stokes H. B., O'Hara C. M., Buchanan R. D., Olson W. H. An improved method for examination of cerebrospinal fluid cells. Neurology. 1975 Oct;25(10):901–906. doi: 10.1212/wnl.25.10.901. [DOI] [PubMed] [Google Scholar]

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