Abstract
We have examined the effects of complement depletion, brought about by cobra venom factor (CVF), on the clearance of radiolabelled BSA from the kidney in acute serum sickness in rabbits. The decomplemented group showed a significant decrease in the rate of clearance of renal-bound antigen with a mean half-clearance time of 19.5 days (range 9.3--60) vs 11.3 (range 8.7--15) for the control group. C3 was rapidly cleared from the glomerulus (as assessed by immunofluorescence) when further deposition was prevented by treatment with CVF. Glomerular immune complexes apparently activated the alternative pathway in vitro. These findings suggest that in immune complex nephritis, the clearance of deposited complexes from the kidney is complement-dependent.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ballow M., Cochrane C. G. Two anticomplementary factors in cobra venom: hemolysis of guinea pig erythrocytes by one of them. J Immunol. 1969 Nov;103(5):944–952. [PubMed] [Google Scholar]
- Bartolotti S. R. Quantitative elution studies in experimental immune complex and nephrotoxic nephritis. Clin Exp Immunol. 1977 Aug;29(2):334–341. [PMC free article] [PubMed] [Google Scholar]
- Cochrane C. G., Koffler D. Immune complex disease in experimental animals and man. Adv Immunol. 1973;16(0):185–264. doi: 10.1016/s0065-2776(08)60298-9. [DOI] [PubMed] [Google Scholar]
- Czop J., Nussenzweig V. Studies on the mechanism of solubilization of immune precipitates by serum. J Exp Med. 1976 Mar 1;143(3):615–630. doi: 10.1084/jem.143.3.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans D. J., Williams D. G., Peters D. K., Sissons J. G., Boulton-Jones J. M., Ogg C. S., Cameron J. S., Hoffbrand B. I. Glomerular deposition of properdin in Henoch-Schönlein syndrome and idiopathic focal nephritis. Br Med J. 1973 Aug 11;3(5875):326–328. doi: 10.1136/bmj.3.5875.326. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fehr J., Jacob H. S. In vitro granulocyte adherence and in vivo margination: two associated complement-dependent functions. Studies based on the acute neutropenia of filtration leukophoresis. J Exp Med. 1977 Sep 1;146(3):641–652. doi: 10.1084/jem.146.3.641. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fish A. J., Michael A. F., Vernier R. L., Good R. A. Acute serum sickness nephritis in the rabbit. An immune deposit disease. Am J Pathol. 1966 Dec;49(6):997–1022. [PMC free article] [PubMed] [Google Scholar]
- LAURELL C. B. ANTIGEN-ANTIBODY CROSSED ELECTROPHORESIS. Anal Biochem. 1965 Feb;10:358–361. doi: 10.1016/0003-2697(65)90278-2. [DOI] [PubMed] [Google Scholar]
- MATSUMOTO T., YAMAMOTO N., MAENO K. Effect of egg passage on the reaction between HVJ (hemagglutinating virus of Japan) and chicken erythrocytes. J Immunol. 1961 Nov;87:590–598. [PubMed] [Google Scholar]
- McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
- Miller G. W., Nussenzweig V. A new complement function: solubilization of antigen-antibody aggregates. Proc Natl Acad Sci U S A. 1975 Feb;72(2):418–422. doi: 10.1073/pnas.72.2.418. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Valdes A. J., Senterfit L. B., Pollack A. D., Germuth F. G., Jr The effect of antigen excess on chronic immune complex glomerulonephritis. Johns Hopkins Med J. 1969 Jan;124(1):9–17. [PubMed] [Google Scholar]
- Wilson C. B., Dixon F. J. Antigen quantitation in experimental immune complex glomerulonephritis. I. Acute serum sickness. J Immunol. 1970 Aug;105(2):279–290. [PubMed] [Google Scholar]