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. 1978 Apr;32(1):169–178.

Enzyme differentiation of lymphocyte subpopulations in sections of human lymph nodes, tonsils and periodontal disease.

G J Seymour, H M Dockrell, J S Greenspan
PMCID: PMC1541303  PMID: 307463

Abstract

Five enzymes have been studied to differentiate between T and B lymphocytes in sections of human lymph nodes, tonsils and chronic inflammatory periodontal disease. The presence of acid phosphatase, beta-glucuronidase N-acetyl-beta-glucosaminidase, non-specific esterase and fluoride-resistant esterase activity was determined histochemically. The results indicate that cells in the B area of both lymph nodes and tonsils are negative for enzyme activity, while those in the T area show a single intense granule of activity. These enzymes were unable to differentiate between T blasts, B blasts and plasma cells in the sections studied. The majority of the lymphoid cells in the lesions of chronic inflammatory periodontal disease are enzyme-negative and probably of B-cell origin.

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