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. 1978 Jun;32(3):387–391.

Immune complexes in peripheral blood polymorphonuclear leucocytes of malignant melanoma patients.

T H The, M van der Giessen, H A Huiges, H Schraffordt Koops, I van Wingerden
PMCID: PMC1541312  PMID: 688694

Abstract

Immune complexes in PMN cells of sixty-five patients with malignant melanoma were studied by scoring the cells for IgG and complement inclusions. Group (a), consisting of thirty-four patients free from tumour after surgical therapy, showed a median IgG score of 17, which was not statistically different from the control group (n = 11), with a median score of 10. However, the tumour-bearing group (b) (n = 31) showed a significantly higher median score of 22. When group (a) was subidivided, it was shown that the patients who had an invasive growing melanoma with a bad prognosis (n = 11) showed a significantly higher median score of 25, while the patients who had a superficial melanoma with good prognosis were not different from the controls. After subdividing group (b), it was shown that patients with localized disease (n = 13) had a median score of 12, which not different from that of the controls. However, the patients with regional lymphnode involvement (n = 9) or distant metastases (n = 9) showed significantly higher median scores of 69 and 24 respectively. Six out of sixteen patients showed a significant drop in PMN inclusions after surgery. Complement scores gave similar patterns.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boggs D. R. The kinetics of neutrophilic leukocytes in health and in disease. Semin Hematol. 1967 Oct;4(4):359–386. [PubMed] [Google Scholar]
  2. COCHRANE C. G., WEIGLE W. O., DIXON F. J. The role of polymorphonuclear leukocytes in the initiation and cessation of the Arthus vasculitis. J Exp Med. 1959 Sep 1;110:481–494. doi: 10.1084/jem.110.3.481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cats A., Lafeber G. J., Klein F. Immunoglobulin phagocytosis by granulocytes from sera and synovial fluids in various rheumatoid and nonrheumatoid diseases. Ann Rheum Dis. 1975 Apr;34(2):146–155. doi: 10.1136/ard.34.2.146. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clark W. H., Jr, From L., Bernardino E. A., Mihm M. C. The histogenesis and biologic behavior of primary human malignant melanomas of the skin. Cancer Res. 1969 Mar;29(3):705–727. [PubMed] [Google Scholar]
  5. Clark W. H., Jr, Mastrangelo M. J., Ainsworth A. M., Berd D., Bellet R. E., Bernardino E. A. Current concepts of the biology of human cutaneous malignant melanoma. Adv Cancer Res. 1977;24:267–338. doi: 10.1016/s0065-230x(08)61017-9. [DOI] [PubMed] [Google Scholar]
  6. Currie G. A., Basham C. Serum mediated inhibition of the immunological reactions of the patient to his own tumour: a possible role for circulating antigen. Br J Cancer. 1972 Dec;26(6):427–438. doi: 10.1038/bjc.1972.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hellström K. E., Hellström I. Lymphocyte-mediated cytotoxicity and blocking serum activity to tumor antigens. Adv Immunol. 1974;18:209–277. doi: 10.1016/s0065-2776(08)60311-9. [DOI] [PubMed] [Google Scholar]
  8. Hersey P., Isbister J., Edwards A., Murray E., Adams E., Biggs J., Milton G. W. Antibody-dependent cell-mediated cytotoxicity against melanoma cells induced by plasmapheresis. Lancet. 1976 Apr 17;1(7964):825–828. doi: 10.1016/s0140-6736(76)90478-5. [DOI] [PubMed] [Google Scholar]
  9. Hoffken K., Meredith I. D., Robins R. A., Baldwin R. W., Davies C. J., Blamey R. W. Circulating immune complexes in patients with breast cancer. Br Med J. 1977 Jul 23;2(6081):218–220. doi: 10.1136/bmj.2.6081.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jansen H. M., The T. H., de Gast G. C., Huiges H. A., Esselink M. T., van der Wal A. M., Orie N. G. Immunoglobulin and complement inclusions in peripheral blood polymorphonuclear leucocytes of patients with bronchial carcinoma. Thorax. 1977 Dec;32(6):706–710. doi: 10.1136/thx.32.6.706. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jerry L. M., Rowden G., Cano P. O., Phillips T. M., Deutsch G. F., Capek A., Hartmann D., Lewis M. G. Immune complexes in human melanoma: a consequence of deranged immune regulation. Scand J Immunol. 1976;5(6-7):845–859. doi: 10.1111/j.1365-3083.1976.tb03034.x. [DOI] [PubMed] [Google Scholar]
  12. Jose D. G., Seshadri R. Circulating immune complexes in human neuroblastoma: direct assay and role in blocking specific cellular immunity. Int J Cancer. 1974 Jun 15;13(6):824–838. doi: 10.1002/ijc.2910130610. [DOI] [PubMed] [Google Scholar]
  13. Ploem J. S. A study of filters and light sources in immunofluorescence microscopy. Ann N Y Acad Sci. 1971 Jun 21;177:414–429. doi: 10.1111/j.1749-6632.1971.tb35070.x. [DOI] [PubMed] [Google Scholar]
  14. Poskitt P. K., Poskitt T. R., Wallace J. H. Renal deposition of soluble immune complexes in mice bearing B-16 melanoma. Characterization of complexes and relationship to tumor progress. J Exp Med. 1974 Aug 1;140(2):410–425. doi: 10.1084/jem.140.2.410. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Samayoa E. A., McDuffie F. C., Nelson A. M., Go V. L., Luthra H. S., Brumfield H. W. Immunoglobulin complexes in sera of patients with malignancy. Int J Cancer. 1977 Jan;19(1):12–17. doi: 10.1002/ijc.2910190103. [DOI] [PubMed] [Google Scholar]
  16. Steffelaar J. W., Ten Kate F. J., Nap M., Swaak A. J., De Graaffreitsma C. B., Van Elven E. H., Feltkamp-Vroom T. M. Immune complex detection by immunofluorescence on polymorphonuclear leucocytes. Clin Exp Immunol. 1977 Mar;27(3):391–396. [PMC free article] [PubMed] [Google Scholar]
  17. The T. H., Feltkamp T. E. Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation. Immunology. 1970 Jun;18(6):865–873. [PMC free article] [PubMed] [Google Scholar]
  18. Theofilopoulos A. N., Andrews B. S., Urist M. M., Morton D. L., Dixon F. J. The nature of immune complexes in human cancer sera. J Immunol. 1977 Aug;119(2):657–663. [PubMed] [Google Scholar]
  19. Theofilopoulos A. N., Wilson C. B., Dixon F. J. The Raji cell radioimmune assay for detecting immune complexes in human sera. J Clin Invest. 1976 Jan;57(1):169–182. doi: 10.1172/JCI108257. [DOI] [PMC free article] [PubMed] [Google Scholar]

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