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. 1978 Jun;32(3):381–386.

Influence of continuous-flow blood separator leucophoresis on in vitro functions of human lymphocytes.

H C Siewertsen, H H Peter, J R Kalden, J Wysk, G Eckert, S Oehl, U W Schäfer, H Deicher
PMCID: PMC1541314  PMID: 688693

Abstract

The influence of continuous-flow blood separator leucophoresis on these in vitro functions of human lymphocytes was studied. In ten separation experiments, lymphocyte functions were examined in the presence and absence of mononuclear phagocytes before, during and at the end of continuous flow leucophoresis. Cell surface markers and effector functions in spontaneous cell-mediated cytotoxicity (SCMC) and antibody-dependent cellular cytotoxicity (ADCC) assays did not change significantly under the conditions of leucophoresis. By contrast, separator lymphocytes exhibited a significantly increased mitogenic response to pokeweed mitogen and PPD. Removal of mononuclear phagocytes corrected for this phenomenon. PHA and Con A responses were not affected by leucophoresis, nor was the number of circulating haemopoietic stem cells (CFU-C) significantly altered during a 4 hr continuous flow blood separator run.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blomgren H. Role of B cells in the expression of the PPD response of human lymphocytes in vitro. Scand J Immunol. 1975 Sep;4(5-6):499–510. doi: 10.1111/j.1365-3083.1975.tb02655.x. [DOI] [PubMed] [Google Scholar]
  2. Buckner D., Graw R. G., Jr, Eisel R. J., Henderson E. S., Perry S. Leukapheresis by continuous flow centrifugation (CFC) in patients with chronic myelocytic leukemia (CML). Blood. 1969 Feb;33(2):353–369. [PubMed] [Google Scholar]
  3. Dicke K. A., Platenburg M. G., van Bekkum D. W. Colony formation in agar: in vitro assay for haemopoietic stem cells. Cell Tissue Kinet. 1971 Sep;4(5):463–477. doi: 10.1111/j.1365-2184.1971.tb01554.x. [DOI] [PubMed] [Google Scholar]
  4. Freireich E. J., Judson G., Levin R. H. Separation and collection of leukocytes. Cancer Res. 1965 Oct;25(9):1516–1520. [PubMed] [Google Scholar]
  5. Herzig G. P., Graw R. G., Jr Granulocyte transfusion for bacterial infections. Prog Hematol. 1975;9:207–228. [PubMed] [Google Scholar]
  6. Jondal M., Holm G., Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972 Aug 1;136(2):207–215. doi: 10.1084/jem.136.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jones A. L. Continuous-flow blood cell separation. Transfusion. 1968 Mar-Apr;8(2):94–103. doi: 10.1111/j.1537-2995.1968.tb02402.x. [DOI] [PubMed] [Google Scholar]
  8. Kalden J. R., Peter H. H., Roubin R., Cesarini J. P. Human peripheral Null lymphocytes. I. Isolation, immunological and functional characterization. Eur J Immunol. 1977 Aug;7(8):537–543. doi: 10.1002/eji.1830070809. [DOI] [PubMed] [Google Scholar]
  9. Kurnick J. E., Robison W. A. Clony growth of human peripherl white blood cells in vitro. Blood. 1971 Feb;37(2):136–141. [PubMed] [Google Scholar]
  10. Lani K., Pfisterer H., Ruppelt W., Bolland H., Stich W. Leuko- und Thrombophorese bei Gesunden mit dem NCI-IBM Blutzellseparator. Klin Wochenschr. 1971 Mar 15;49(6):327–332. doi: 10.1007/BF01496452. [DOI] [PubMed] [Google Scholar]
  11. MacLennan I. C. Antibody in the induction and inhibition of lymphocyte cytotoxicity. Transplant Rev. 1972;13:67–90. doi: 10.1111/j.1600-065x.1972.tb00060.x. [DOI] [PubMed] [Google Scholar]
  12. Mellstedt H. In vitro activation of human T and B lymphocytes by pokeweed mitogen. Clin Exp Immunol. 1975 Jan;19(1):75–82. [PMC free article] [PubMed] [Google Scholar]
  13. Oehl S., Schaefer U. W., Boecker W. R., Kalden J. R., Peter H. H. Human peripheral blood "null-lymphocytes", a highly enriched pool for granulocytic stem cells (CFU-C). Z Immunitatsforsch Immunobiol. 1977 Feb;152(5):423–430. [PubMed] [Google Scholar]
  14. Oldham R. K., Dean J. H., Cannon G. B., Ortaldo J. R., Dunston G., Applebaum F., McCoy J. L., Djeu J., Herberman R. B. Cryopreservation of human lymphocyte function as measured by in vitro assays. Int J Cancer. 1976 Aug 15;18(2):145–155. doi: 10.1002/ijc.2910180203. [DOI] [PubMed] [Google Scholar]
  15. Oon C. J., Hobbs J. R. Clinical applications of the continuous flow blood separator machine. Clin Exp Immunol. 1975 Apr;20(1):1–16. [PMC free article] [PubMed] [Google Scholar]
  16. Pentycross C. R., Walden P. A., Bagshawe K. D. Collection of lymphocytes on a continuous-flow blood cell separator. Vox Sang. 1976;31(6):446–450. doi: 10.1111/j.1423-0410.1976.tb04460.x. [DOI] [PubMed] [Google Scholar]
  17. Peter H. H., Knoop F., Kalden J. R. Spontaneous and antibody-dependent cellular cytotoxicity in melanoma patients and healthy control presons. Z Immunitatsforsch Exp Klin Immunol. 1976 Apr;151(3):263–281. [PubMed] [Google Scholar]
  18. Peter H. H., Pavie-Fischer J., Fridman W. H., Aubert C., Cesarini J. P., Roubin R., Kourilsky F. M. Cell-mediate cytotoxicity in vitro of human lymphocytes against a tissue culture melanoma cell line (igr3). J Immunol. 1975 Aug;115(2):539–548. [PubMed] [Google Scholar]
  19. Pross H. F., Jondal M. Cytotoxic lymphocytes from normal donors. A functional marker of human non-T lymphocytes. Clin Exp Immunol. 1975 Aug;21(2):226–235. [PMC free article] [PubMed] [Google Scholar]
  20. Segel G. B., Lichtman M. A., Gordon B. R., MacPherson J. L., Nusbacher J. Plateletpheresis residues: a source of large quantities of human blood lymphocytes. Transfusion. 1976 Sep-Oct;16(5):455–459. doi: 10.1046/j.1537-2995.1976.16577039302.x. [DOI] [PubMed] [Google Scholar]
  21. West W. H., Cannon G. B., Kay H. D., Bonnard G. D., Herberman R. B. Natural cytotoxic reactivity of human lymphocytes against a myeloid cell line: characterization of effector cells. J Immunol. 1977 Jan;118(1):355–361. [PubMed] [Google Scholar]

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