Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1978 Jun;32(3):554–562.

Inhibition of polyclonal B-cell activation by suppressor monocytes in patients with sarcoidosis.

P Katz, A S Fauci
PMCID: PMC1541315  PMID: 357055

Abstract

The present study employed a direct plaque-forming cell (PGC) assay following pokeweed mitogen (PWM) induced polyclonal activation of B lymphocytes in sarcoidosis to evaluate the in vitro humoral immune response in this disease and to delineate the immunoregulation of this response. Sarcoidosis lymphocytes had a suppressed PFC response to polyclonal activation, but were unable to suppress normal B-cell PFC responses in allogeneic co-cultures. Removal of a cell type, which was corticosteroid-resistant, radio-resistant, adherent and a non-T cell, from sarcoidosis mononuclear cell suspensions reversed the suppressed PFC response, indicating the presence of a suppressor monocyte. Thus in vitro suppressor cell activity has now been demonstrated in this disease, which is characterized by multiple immunological aberrancies.

Full text

PDF
554

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berlinger N. T., Lopez C., Good R. A. Facilitation or attenuation of mixed leukocyte culture responsiveness by adherent cells. Nature. 1976 Mar 11;260(5547):145–146. doi: 10.1038/260145a0. [DOI] [PubMed] [Google Scholar]
  2. Broder S., Humphrey R., Durm M., Blackman M., Meade B., Goldman C., Strober W., Waldmann T. Impaired synthesis of polyclonal (non-paraprotein) immunoglobulins by circulating lymphocytes from patients with multiple myeloma Role of suppressor cells. N Engl J Med. 1975 Oct 30;293(18):887–892. doi: 10.1056/NEJM197510302931801. [DOI] [PubMed] [Google Scholar]
  3. Douglas S. D., Schmidt M. E., Siltzbach L. E. Monocyte receptor activity in normal individuals and patients with sarcoidosis. Immunol Commun. 1972;1(1):25–38. doi: 10.3109/08820137209022890. [DOI] [PubMed] [Google Scholar]
  4. Fauci A. S., Balow J. E., Pratt K. R. Human bone marrow lymphocytes. Cytotoxic effector cells in the bone marrow of normal individuals. J Clin Invest. 1976 Apr;57(4):826–835. doi: 10.1172/JCI108358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fauci A. S., Pratt K. R. Activation of human B lymphocytes. I. Direct plaque-forming cell assay for the measurement of polyclonal activation and antigenic stimulation of human B lymphocytes. J Exp Med. 1976 Sep 1;144(3):674–684. doi: 10.1084/jem.144.3.674. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fauci A. S., Pratt K. R. Polyclonal activation of bone-marrow-derived lymphocytes from human peripheral blood measured by a direct plaque-forming cell assay. Proc Natl Acad Sci U S A. 1976 Oct;73(10):3676–3679. doi: 10.1073/pnas.73.10.3676. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fauci A. S., Pratt K. R., Whalen G. Activation of human B lymphocytes. II. Cellular interactions in the PFC response of human tonsillar and peripheral blood B lymphocytes to polyclonal activation by pokeweed mitogen. J Immunol. 1976 Dec;117(6):2100–2104. [PubMed] [Google Scholar]
  8. Fauci A. S., Pratt K. R., Whalen G. Activation of human B lymphocytes. IV. Regulatory effects of corticosteroids on the triggering signal in the plaque-forming cell response of human peripheral blood B lymphocytes to polyclonal activation. J Immunol. 1977 Aug;119(2):598–603. [PubMed] [Google Scholar]
  9. HIRSCHHORN K., SCHREIBMAN R. R., BACH F. H., SILTZBACH L. E. IN-VITRO STUDIES OF LYMPHOCYTES FROM PATIENTS WITH SARCOIDOSIS AND LYMPHOPROLIFERATIVE DISEASES. Lancet. 1964 Oct 17;2(7364):842–843. doi: 10.1016/s0140-6736(64)90691-9. [DOI] [PubMed] [Google Scholar]
  10. Haynes B. F., Fauci A. S. Activation of human B lymphocytes. III. Concanavalin A-induced generation of suppressor cells of the plaque-forming cell response of normal human B lymphocytes. J Immunol. 1977 Jun;118(6):2281–2287. [PubMed] [Google Scholar]
  11. Hedfors E., Norberg R. Evidence for circulating immune complexes in sarcoidosis. Clin Exp Immunol. 1974 Mar;16(3):493–496. [PMC free article] [PubMed] [Google Scholar]
  12. Hirshaut Y., Glade P., Vieira B. D., Ainbender E., Dvorak B., Siltzbach L. E. Sarcoidosis, another disease associated with serologic evidence for herpes-like virus infection. N Engl J Med. 1970 Sep 3;283(10):502–506. doi: 10.1056/NEJM197009032831002. [DOI] [PubMed] [Google Scholar]
  13. Jondal M., Holm G., Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972 Aug 1;136(2):207–215. doi: 10.1084/jem.136.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Jones J. V. Development of sensitivity oto dinitrochlorobenzene in patients with sarcoidosis. Clin Exp Immunol. 1967 Jul;2(4):477–487. [PMC free article] [PubMed] [Google Scholar]
  15. Kirchner H., Chused T. M., Herberman R. B., Holden H. T., Lavrin D. H. Evidence of suppressor cell activity in spleens of mice bearing primary tumors induced by Moloney sarcoma virus. J Exp Med. 1974 Jun 1;139(6):1473–1487. doi: 10.1084/jem.139.6.1473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Laughter A. H., Twomey J. J. Suppression of lymphoproliferation by high concentrations of normal human mononuclear leukocytes. J Immunol. 1977 Jul;119(1):173–179. [PubMed] [Google Scholar]
  17. Moretta L., Webb S. R., Grossi C. E., Lydyard P. M., Cooper M. D. Functional analysis of two human T-cell subpopulations: help and suppression of B-cell responses by T cells bearing receptors for IgM or IgG. J Exp Med. 1977 Jul 1;146(1):184–200. doi: 10.1084/jem.146.1.184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Oreskes I., Siltzbach L. E. Changes in rheumatoid factor activity during the course of sarcoidosis. Am J Med. 1968 Jan;44(1):60–67. doi: 10.1016/0002-9343(68)90237-4. [DOI] [PubMed] [Google Scholar]
  19. Schmidt M. E., Douglas S. D. Monocyte IgG receptor activity, dynamics, and modulation-normal individuals and patients with granulomatous diseases. J Lab Clin Med. 1977 Feb;89(2):332–340. [PubMed] [Google Scholar]
  20. Siegal F. P., Siegal M. Enhancement by irradiated T cells of human plasma cell production: dissection of helper and suppressor functions in vitro. J Immunol. 1977 Feb;118(2):642–647. [PubMed] [Google Scholar]
  21. Stobo J. D., Paul S., Van Scoy R. E., Hermans P. E. Suppressor thymus-derived lymphocytes in fungal infection. J Clin Invest. 1976 Feb;57(2):319–328. doi: 10.1172/JCI108283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Twomey J. J., Laughter A. H., Farrow S., Douglass C. C. Hodgkin's disease. An immunodepleting and immunosuppressive disorder. J Clin Invest. 1975 Aug;56(2):467–475. doi: 10.1172/JCI108113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Waldmann T. A., Durm M., Broder S., Blackman M., Blaese R. M., Strober W. Role of suppressor T cells in pathogenesis of common variable hypogammaglobulinaemia. Lancet. 1974 Sep 14;2(7881):609–613. doi: 10.1016/s0140-6736(74)91940-0. [DOI] [PubMed] [Google Scholar]
  24. Weiner M. S., Bianco C., Nussenzweig V. Enhanced binding of neuraminidase-treated sheep erythrocytes to human T lymphocytes. Blood. 1973 Dec;42(6):939–946. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES