Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1978 Jun;32(3):392–398.

Haemolytic complement in peripheral lymph of normal men.

W L Olszewski, A Engeset
PMCID: PMC1541330  PMID: 99278

Abstract

The haemolytic activity of nine individual components of complement and the concentrations of C1q, C1s, C4, C3, C3PA and C9 proteins were measured in the leg lymph and serum of four normal men. The mean lymph/serum ratio for total haemolytic complement was 0.257, for C1H50 it was 0.138, for C4H50 0.105, for C2H50 0.279, for C3H50 0.063, for C5H50 0.266, for C6H50 0.145, for C7H50 0.25, for C8H50 0.244 and for C9H50 0.253. The mean lymph/serum ratio for complement proteins was: for C1q 0.048, for C1s 0.06, for C4 0.199, for C3 0.225, for C9 0.244 and for C3PA 0.263. The low haemolytic activity of total complement and of all components in lymph seems to be dependent on the low complement protein concentration. The remarkably low lymph C1q protein concentration may play a physiological role in controlling total complement activity in lymph and interstitial fluid. The total complement haemolytic activity in lymph exceeded the haemolytic activity of C1 and C3, which may indicate the possibility of independent activation of C5-7 and C8-9. The considerably low C3H50 level, with high C3 protein concentration, at present lacks a proper explanation.

Full text

PDF
392

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bergström K., Werner B. Proteins in human thoracic duct lymph. Studies on the distribution of some proteins between lymph and blood. Acta Chir Scand. 1966 May;131(5):413–422. [PubMed] [Google Scholar]
  2. Brown P. C., Glynn L. E., Holborow E. J. The dual necessity for delayed hypersensitivity and circulating antibody in the pathogenesis of experimental allergic orchitis in guinea-pigs. Immunology. 1967 Sep;13(3):307–314. [PMC free article] [PubMed] [Google Scholar]
  3. Day N. K., Geiger H., Stroud R., DeBracco M., Mancaido B., Windhorst D., Good R. A. C1r deficiency: an inborn error associated with cutaneous and renal disease. J Clin Invest. 1972 May;51(5):1102–1108. doi: 10.1172/JCI106902. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Engeset A., Hager B., Nesheim A., Kolbenstvedt A. Studies on human peripheral lymph. I. Sampling method. Lymphology. 1973 Mar;6(1):1–5. [PubMed] [Google Scholar]
  5. Engeset A., Olszewski W., Jaeger P. M., Sokolowski J., Theodorsen L. Twenty-four hour variation in flow and composition of leg lymph in normal men. Acta Physiol Scand. 1977 Feb;99(2):140–148. doi: 10.1111/j.1748-1716.1977.tb10364.x. [DOI] [PubMed] [Google Scholar]
  6. Fearon D. T., Ruddy S., Schur P. H., McCabe W. R. Activation of the properdin pathway of complement in patients with gram-negative of bacteremia. N Engl J Med. 1975 May 1;292(18):937–940. doi: 10.1056/NEJM197505012921802. [DOI] [PubMed] [Google Scholar]
  7. Garlick D. G., Renkin E. M. Transport of large molecules from plasma to interstitial fluid and lymph in dogs. Am J Physiol. 1970 Dec;219(6):1595–1605. doi: 10.1152/ajplegacy.1970.219.6.1595. [DOI] [PubMed] [Google Scholar]
  8. Jullien-Vitoux D., Voisin G. A. Studies in vascular permeability. II. Comparative extravasation of different immunoglobulin classes in normal guinea pig skin. Eur J Immunol. 1973 Nov;3(11):663–667. doi: 10.1002/eji.1830031102. [DOI] [PubMed] [Google Scholar]
  9. Lachmann P. J., Halbwachs L. The influence of C3b inactivator (KAF) concentration on the ability of serum to support complement activation. Clin Exp Immunol. 1975 Jul;21(1):109–114. [PMC free article] [PubMed] [Google Scholar]
  10. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  11. McClelland D. B., Furth R. V. In vitro synthesis of beta1C/beta1A globulin (the C3 component of complement) by tissues and leucocytes of mice. Immunology. 1976 Dec;31(6):855–861. [PMC free article] [PubMed] [Google Scholar]
  12. NISHIOKA K. Measurements of complement by agglutination of human erythrocytes reacting in immune-adherence. J Immunol. 1963 Jan;90:86–97. [PubMed] [Google Scholar]
  13. Nelson R. A., Jr, Biro C. E. Complement components of a haemolytically deficient strain of rabbits. Immunology. 1968 Apr;14(4):527–540. [PMC free article] [PubMed] [Google Scholar]
  14. Olszewski W. L., Engeset A., Lukasiewicz H. Immunoglobulins, complement and lysozyme in leg lymph of normal men. Scand J Clin Lab Invest. 1977 Dec;37(8):669–674. doi: 10.3109/00365517709101847. [DOI] [PubMed] [Google Scholar]
  15. Olszewski W., Engeset A., Jaeger P. M., Sokolowski J., Theodorsen L. Flow and composition of leg lymph in normal men during venous stasis, muscular activity and local hyperthermia. Acta Physiol Scand. 1977 Feb;99(2):149–155. doi: 10.1111/j.1748-1716.1977.tb10365.x. [DOI] [PubMed] [Google Scholar]
  16. Sjöholm A. G. Complement components in normal serum and plasma quantitated by electroimmunoassay. Scand J Immunol. 1975;4(1):25–30. doi: 10.1111/j.1365-3083.1975.tb02596.x. [DOI] [PubMed] [Google Scholar]
  17. Taylor A. E., Gibson W. H., Granger H. J., Guyton A. C. The interaction between intracapillary and tissue forces in the overall regulation of interstitial fluid volume. Lymphology. 1973 Dec;6(4):192–208. [PubMed] [Google Scholar]
  18. Taylor A., Gibson H. Concentrating ability of lymphatic vessels. Lymphology. 1975 Jun;8(2):43–49. [PubMed] [Google Scholar]
  19. Teisberg P. The conversion rate of human C3 under different storage conditions. Vox Sang. 1971 Mar;20(3):230–238. doi: 10.1111/j.1423-0410.1971.tb00434.x. [DOI] [PubMed] [Google Scholar]
  20. Thompson R. A., Rowe D. S. Reactive haemolysis--a distinctive form of red cell lysis. Immunology. 1968 May;14(5):745–762. [PMC free article] [PubMed] [Google Scholar]
  21. Vroon D. H., Schultz D. R., Zarco R. M. The separation of nine components and two inactivators of components of complement in humansserum. Immunochemistry. 1970 Jan;7(1):43–61. doi: 10.1016/0019-2791(70)90029-7. [DOI] [PubMed] [Google Scholar]
  22. Wellek B., Opferkuch W. A case of deficiency of the seventh component of complement in man. Biological properties of a C7-deficient serum and description of a C7-inactivating principle. Clin Exp Immunol. 1975 Feb;19(2):223–235. [PMC free article] [PubMed] [Google Scholar]
  23. Wyatt H. V., Colten H. R., Borsos T. Production of the second (C2) and fourth (C4) components of guinea pig complement by single peritoneal cells: evidence that one cell may produce both components. J Immunol. 1972 Jun;108(6):1609–1614. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES