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. 1978 Jun;32(3):510–516.

Nephrotoxic potency of antisera to three rat glomerular basement membrane glycoproteins.

B Devulder, P Bardos, B Plouvier, J C Martin, J P Muh, A Tacquet
PMCID: PMC1541335  PMID: 357054

Abstract

In a previous article, we cited studies which have allowed us to isolate diverse glycoproteins of the rat glomerular basement membrane (GMB) and to study their biochemical structures and antigenicity. This present study attempts to examine, using the heterologous nephrotoxic nephritis model (Masugi's nephritis) the nephrotoxicity of immune sera prepared from three of these glycoproteins: one fairly rich in collagen-like structures (A3), another lacking collagen-like structures (A1), and a third of intermediate composition (A2). The results obtained are discussed in relation to those already published concerning the nature of the GBM antigen(s) responsible for the nephrotoxicity of the sera.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bardos P., Lanson M., Degand P., Gutman N., Garrigue M. A., Muh J. P. Study of glomerular basement membrane glycoproteins resistant to hydrolysis by collagenase. FEBS Lett. 1976 May 1;64(2):385–390. doi: 10.1016/0014-5793(76)80333-x. [DOI] [PubMed] [Google Scholar]
  2. Bardos P., Lanson M., Degand P., Muh J. P. Etude biochimique des glycoprotéides de membrane basale glomérulaire du rein humain solubilisés par la collagénase. Clin Chim Acta. 1973 Sep 28;48(1):27–35. doi: 10.1016/0009-8981(73)90214-3. [DOI] [PubMed] [Google Scholar]
  3. Boesken W. H., Hammer D. K. Purification and chemical characterization of a basement membrane glycoprotein present in the urine of nephritic rabbits. Hoppe Seylers Z Physiol Chem. 1972 Sep;353(9):1429–1439. doi: 10.1515/bchm2.1972.353.2.1429. [DOI] [PubMed] [Google Scholar]
  4. COONS A. H., KAPLAN M. H. Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody. J Exp Med. 1950 Jan 1;91(1):1–13. doi: 10.1084/jem.91.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DUNCAN D. A., DRUMMOND K. N., MICHAEL A. F., VERNIER R. L. PULMONARY HEMORRHAGE AND GLOMERULONEPHRITIS. REPORT OS SIX CASES AND STUDY OF THE RENAL LESION BY THE FLUORESCENT ANTIBODY TECHNIQUE AND ELECTRON MICROSCOPY. Ann Intern Med. 1965 May;62:920–938. doi: 10.7326/0003-4819-62-5-920. [DOI] [PubMed] [Google Scholar]
  6. Devulder B., Bardos P., Plouvier B., Gerfaux J., Muh J. P., Tacquet A. Etude de la néphrotoxicité d'anticorps anti-glycoprotéines de membrane basale glomérulaire du rat Wistar. C R Acad Sci Hebd Seances Acad Sci D. 1976 Nov 8;283(11 500):1373–1376. [PubMed] [Google Scholar]
  7. Hartmann L., Ollier M. P., Lagrue G. Néphrite hétéro-immune de Masugi chez le rat. I. Syndrome lipido-protidique. Ann Biol Clin (Paris) 1969 Jul-Sep;27(7):407–423. [PubMed] [Google Scholar]
  8. Hawking K. M., Milgrom F. Basement membrane antigens combining with antisera to human glomerular asement membrane and lymphocytes. Int Arch Allergy Appl Immunol. 1972;43(5):641–656. doi: 10.1159/000230881. [DOI] [PubMed] [Google Scholar]
  9. Hirbec G., Gouygou C., Lagrue G. Une nouvelle coloration histologique: l'hexaméthylènététramine argentique-chromotrope. Ses résultats en pathologie rénale. Sem Hop. 1974 Feb 14;50(8):523–529. [PubMed] [Google Scholar]
  10. Huang F., Kalant N. Isolation and characterization of antigenic components of rat glomerular basement membrane. Can J Biochem. 1968 Dec;46(12):1523–1532. doi: 10.1139/o68-226. [DOI] [PubMed] [Google Scholar]
  11. KRAKOWER C. A., GREENSPON S. A. Localization of the nephrotoxic antigen within the isolated renal glomerulus. AMA Arch Pathol. 1951 Jun;51(6):629–639. [PubMed] [Google Scholar]
  12. Kefalides N. A. Structure and biosynthesis of basement membranes. Int Rev Connect Tissue Res. 1973;6:63–104. doi: 10.1016/b978-0-12-363706-2.50008-8. [DOI] [PubMed] [Google Scholar]
  13. Levin A., Killander D., Klein E., Nordenskjöld B., Inoue M. Applications of microspectofluorometry in quantitation of immunofluorescence on single cells. Ann N Y Acad Sci. 1971 Jun 21;177:481–489. doi: 10.1111/j.1749-6632.1971.tb35078.x. [DOI] [PubMed] [Google Scholar]
  14. Marquardt H., Wilson C. B., Dixon F. J. Isolation and immunological characterization of human glomerular basement membrane antigens. Kidney Int. 1973 Feb;3(2):57–65. doi: 10.1038/ki.1973.12. [DOI] [PubMed] [Google Scholar]
  15. McIntosh R. M., Griswold W. Antigen identification in Goodpasture's syndrome. Arch Pathol. 1971 Nov;92(5):329–333. [PubMed] [Google Scholar]
  16. Misra R. P. Glomerular basement membrane antigens of Masugi nephritis. Immunology. 1973 Dec;25(6):967–980. [PMC free article] [PubMed] [Google Scholar]
  17. Mohos S. C., Skoza L. Glomerular sialoprotein. Science. 1969 Jun 27;164(3887):1519–1521. doi: 10.1126/science.164.3887.1519. [DOI] [PubMed] [Google Scholar]
  18. Mohos S. C., Skoza L. Variations in the sialic acid concentration of glomerular basement membrane preparations obtained by ultrasonic treatment. J Cell Biol. 1970 May;45(2):450–455. doi: 10.1083/jcb.45.2.450. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nagi A. H., Barabas A. Z., Alexander F., Lannigan R. Induction of progressive glomerulonephritis in rats by eluted -globulin. Br J Exp Pathol. 1971 Aug;52(4):371–377. [PMC free article] [PubMed] [Google Scholar]
  20. Nicholes B. K., Krakower C. A., Greenspon S. A. The chemically isolated lamina densa of the renal glomerulus. Proc Soc Exp Biol Med. 1973 Apr;142(4):1316–1321. doi: 10.3181/00379727-142-37231. [DOI] [PubMed] [Google Scholar]
  21. Passos H. C., Siqueira M., Martinez O. C., Bier O. G. Studies on the nephrotoxic activity of guinea-pig gamma-1 and gamma-2 antibodies. Immunology. 1974 Feb;26(2):407–416. [PMC free article] [PubMed] [Google Scholar]
  22. Rothbard S., Watson R. F. Comparison of reactions of antibodies to rat collagen and to rat kidney in the basement membranes of rat renal glomeruli. J Exp Med. 1969 Jun 1;129(6):1145–1161. doi: 10.1084/jem.129.6.1145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shibata S., Miyakawa Y., Naruse T., Nagasawa T., Takuma T. A glycoprotein that induces nephrotoxic antibody: its isolation and purification from rat glomerular basement membrane. J Immunol. 1969 Mar;102(3):593–601. [PubMed] [Google Scholar]
  24. Skoza L., Mohos S. C. Nephritogenic antigenicity of chromatographic fractions of trypsin and pronase glomerular digests. Proc Soc Exp Biol Med. 1969 Sep;131(4):1189–1193. doi: 10.3181/00379727-131-34067. [DOI] [PubMed] [Google Scholar]
  25. Sternberg M., Lagrue G. Role hapténique et métabolisme des unités disaccharidiques de la membrane basale glomérulaire au cours de la glomérulo-néphrite par anticorps néphrotoxiques hétérologues chez le rat. Ann Immunol (Paris) 1977 Jul-Oct;128C(4-5):905–908. [PubMed] [Google Scholar]
  26. UNANUE E. R., DIXON F. J. EXPERIMENTAL GLOMERULONEPHRITIS. V. STUDIES ON THE INTERACTION OF NEPHROTOXIC ANTIBODIES WITH TISSUE OF THE RAT. J Exp Med. 1965 May 1;121:697–714. doi: 10.1084/jem.121.5.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Unanue E. R., Dixon F. J. Experimental glomerulonephritis: immunological events and pathogenetic mechanisms. Adv Immunol. 1967;6:1–90. doi: 10.1016/s0065-2776(08)60521-0. [DOI] [PubMed] [Google Scholar]
  28. Vogt A., Bockhorn H., Kozima K., Sasaki M. Electron microscopic localization of the nephrotoxic antibody in the glomeruli of the rat after intravenous application of purified nephritogenic antibody-ferritin conjugates. J Exp Med. 1968 May 1;127(5):867–878. doi: 10.1084/jem.127.5.867. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Westberg N. G., Michael A. F. Human glomerular basement membrane. Preparation and composition. Biochemistry. 1970 Sep 15;9(19):3837–3846. doi: 10.1021/bi00821a025. [DOI] [PubMed] [Google Scholar]
  30. Westberg N. G., Michael A. F. Human glomerular basement membrane: chemical composition in diabetes mellitus. Acta Med Scand. 1973 Jul-Aug;1-2(1):39–47. doi: 10.1111/j.0954-6820.1973.tb19411.x. [DOI] [PubMed] [Google Scholar]

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