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. 1976 Aug;25(2):227–233.

Sialoproteinaemia: lack of correlation with inhibition of in vitro lymphoblastosis induced by phytohaemagglutinin or alloantigen.

B N Gray, R R Kopito, L L Anderson, O L Baralt, C K Connery, E Watkins Jr
PMCID: PMC1541366  PMID: 133776

Abstract

Elevation of serum-bound sialic acid concentration in different disease states fails to correlate significantly with suppressive serum actions in mixed allogeneic lymphocyte cultures or phyto-haemagglutinin cultures. Heat-decomplemented serum from patients with abnormal levels of bound sialic acid was added to parallel cultures containing similar blood lymphocyte populations derived from normal humans. Wide fluctuations of the rate of incorporation of tritiated thymidine into nucleoprotein indicated presence of suppressive elements other than sialoprotein in the added serum components. Serum with rising sialyl concentration derived from patients with cancer showed slight tendency to augment mixed lymphocyte and phytohaemagglutinin responses. The findings suggest that the previously documented nonspecific suppressive action of serum sialoprotein on human host lymphoblastic response to neuraminidase-treated cancer cells represents a mechanism unique to that culture system rather than a manifestation of a general immunoregulatory function of serum sialoprotein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Apffel C. A., Peters J. H. Regulation of antigenic expression. J Theor Biol. 1970 Jan;26(1):47–59. doi: 10.1016/s0022-5193(70)80031-5. [DOI] [PubMed] [Google Scholar]
  2. Apffel C. A., Peters J. H. Tumors and serum glycoproteins. The 'symbodies'. Prog Exp Tumor Res. 1969;12:1–54. [PubMed] [Google Scholar]
  3. Ashikawa K., Inoue K., Shimizu T., Ishibashi Y. An increase of serum alpha-globulin in tumor-bearing hosts and its immunological significance. Jpn J Exp Med. 1971 Aug;41(4):339–355. [PubMed] [Google Scholar]
  4. Bogden A. E., Brown M. D., Neville G. A., Gray M. Primary (methylcholanthrene-induced) fibrosarcomas and glycoprotein synthesis. Cancer Res. 1967 Feb;27(2):230–236. [PubMed] [Google Scholar]
  5. Burger D. R., Lilley D. P., Vetto R. M. Lymphocyte suppression associated with alpha globulin. Changes in cellular immunity. Cell Immunol. 1974 Mar 15;10(3):432–441. doi: 10.1016/0008-8749(74)90134-8. [DOI] [PubMed] [Google Scholar]
  6. Chase P. S. The effects of human serum fractions on phytohemagglutinin- and concanavalin A-stimulated human lymphocyte cultures. Cell Immunol. 1972 Dec;5(4):544–554. doi: 10.1016/0008-8749(72)90104-9. [DOI] [PubMed] [Google Scholar]
  7. Cooperband S. R., Bondevik H., Schmid K., Mannick J. A. Transformation of human lymphocytes: inhibition by homologous alpha globulin. Science. 1968 Mar 15;159(3820):1243–1244. doi: 10.1126/science.159.3820.1243. [DOI] [PubMed] [Google Scholar]
  8. El-Ghaffar Y. A., Assad S. Serum glycoprotein level at different stages of tumour growth. Br J Cancer. 1967 Sep;21(3):601–605. doi: 10.1038/bjc.1967.70. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gray B. N., Watkins E., Jr Immunologic approach to cancer therapy. Med Clin North Am. 1975 Mar;59(2):327–337. doi: 10.1016/s0025-7125(16)32035-1. [DOI] [PubMed] [Google Scholar]
  10. Hsu C. C., Leevy C. M. Inhibition of PHA-stimulated lymphocyte transformation by plasma from patients with advanced alcoholic cirrhosis. Clin Exp Immunol. 1971 May;8(5):749–760. [PMC free article] [PubMed] [Google Scholar]
  11. Hsu C. C., LoGerfo P. Correlation between serum alpha-globulin and plasma inhibitory effect on PHA-stimulated lymphocytes in colon cancer patients. Proc Soc Exp Biol Med. 1972 Feb;139(2):575–578. doi: 10.3181/00379727-139-36189. [DOI] [PubMed] [Google Scholar]
  12. MARTENSSON E., RAAL A., SVENNERHOLM L. Sialic acids in blood serum. Biochim Biophys Acta. 1958 Oct;30(1):124–129. doi: 10.1016/0006-3002(58)90248-8. [DOI] [PubMed] [Google Scholar]
  13. MCNEIL C., BERRETT C. R., SU L. Y., TRENTELMAN E. F., HELMICK W. M. SIALIC ACID AS A MEASURE OF SERUM MUCOPROTEINS. Am J Clin Pathol. 1965 Feb;43:130–133. doi: 10.1093/ajcp/43.2.130. [DOI] [PubMed] [Google Scholar]
  14. Nimberg R. B., Glasgow A. H., Menzoian J. O., Constantian M. B., Cooperband S. R., Mannick J. A., Schmid K. Isolation of an immunosuppressive peptide fraction from the serum of cancer patients. Cancer Res. 1975 Jun;35(6):1489–1494. [PubMed] [Google Scholar]
  15. SAIFER A., GERSTENFELD S. Photometric determination of sialic acids in serum and cerebrospinal fluid with the thiobarbituric acid method. Clin Chim Acta. 1962 Jul;7:467–475. doi: 10.1016/0009-8981(62)90086-4. [DOI] [PubMed] [Google Scholar]
  16. Silk M. R. The effect of uremic plasma on lymphocyte transformation. Invest Urol. 1967 Sep;5(2):195–199. [PubMed] [Google Scholar]
  17. WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]
  18. WEIMER H. E., QUINN F. A., REDLICHMOSHIN J., NISHIHARA H. Effects of tumor growth on serum glycoprotein concentration in the rat. J Natl Cancer Inst. 1957 Sep;19(3):409–417. doi: 10.1093/jnci/19.3.409. [DOI] [PubMed] [Google Scholar]
  19. WINZLER R. J. Determination of serum glycoproteins. Methods Biochem Anal. 1955;2:279–311. doi: 10.1002/9780470110188.ch10. [DOI] [PubMed] [Google Scholar]
  20. Wands J. R., Perrotto J. L., Alpert E., Isselbacher K. J. Cell-mediated immunity in acute and chronic hepatitis. J Clin Invest. 1975 May;55(5):921–929. doi: 10.1172/JCI108021. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Watkins E., Jr, Gray B. N., Anderson L. L., Baralt O. L., Nebril L. R., Waters M. F., Connery C. K. Neuraminidase-mediated augmentation of in vitro immune response of patients with solid tumors. Int J Cancer. 1974 Dec 15;14(6):799–807. doi: 10.1002/ijc.2910140614. [DOI] [PubMed] [Google Scholar]
  22. Whang H. Y., Chun D., Neter E. Immunosuppression by antigen-associated human serum alpha-one protein. J Immunol. 1969 Oct;103(4):824–827. [PubMed] [Google Scholar]
  23. Zacharia T. P., Pollard M. Elevated levels of alpha-globulins in sera from germfree rats with 3-methylcholanthrene-induced tumors. J Natl Cancer Inst. 1969 Jan;42(1):35–38. [PubMed] [Google Scholar]

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