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. 1976 Jul;25(1):50–58.

Studies on J chain and binding site for secretory component in circulating human B cells.

P Brandtzaeg
PMCID: PMC1541377  PMID: 62630

Abstract

Lymphocyte-enriched mononuclear cell fractions were obtained from the peripheral blood of four healthy young adults. Living B cells with membrane immunoglobulin (Ig) were studied by immunofluorescence to reveal exposed J-chain determinants or surface affinity for the secretory component (SC). Alcohol-fixed cell smears were similarly studied with and without prior denaturation in acid urea. It was concluded that J chain-containing polymeric Ig of endogenous origin is generally not present on the surface of circulating B cells. If occasional cells bear IgM or IgA polymers rather than monomers, their SC-binding site must be concealed to a degree that it is not functional. Affinity for SC is therefore unlikely to be involved in a selective homing of IgM- and IgA-immunocyte precursors from peripheral blood to glandular regions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beh K. J., Lascelles A. K. Class specificity of intracellular and surface immunoglobulin of cells in popliteal and intestinal lymph from sheep. Aust J Exp Biol Med Sci. 1974 Jun;52(Pt 3):505–514. doi: 10.1038/icb.1974.49. [DOI] [PubMed] [Google Scholar]
  2. Brandtzaeg P., Baklien K. Immunohistochemical studies of the formation and epithelial transport of immunoglobulins in normal and diseased human intestinal mucosa. Scand J Gastroenterol Suppl. 1976;36:1–45. [PubMed] [Google Scholar]
  3. Brandtzaeg P. Blocking effect of J chain and J-chain antibody on the binding of secretory component to human IgA and IgM. Scand J Immunol. 1975;4(8):837–842. doi: 10.1111/j.1365-3083.1975.tb03725.x. [DOI] [PubMed] [Google Scholar]
  4. Brandtzaeg P. Characteristics of SC-Ig complexes formed in vitro. Adv Exp Med Biol. 1974;45(0):87–97. doi: 10.1007/978-1-4613-4550-3_10. [DOI] [PubMed] [Google Scholar]
  5. Brandtzaeg P. Human secretory component. I. Purification of free secretory component from colostrum. Scand J Immunol. 1974;3(5):579–588. doi: 10.1111/j.1365-3083.1974.tb01291.x. [DOI] [PubMed] [Google Scholar]
  6. Brandtzaeg P. Immunochemical studies on free and bound J chain of human IgA and IgM. Scand J Immunol. 1975 Sep;4(5-6):439–450. doi: 10.1111/j.1365-3083.1975.tb02649.x. [DOI] [PubMed] [Google Scholar]
  7. Brandtzaeg P. Mucosal and glandular distribution of immunoglobulin components. Immunohistochemistry with a cold ethanol-fixation technique. Immunology. 1974 Jun;26(6):1101–1114. [PMC free article] [PubMed] [Google Scholar]
  8. Brandtzaeg P. Purification of J chain after mild reduction of human immunoglobulins. Scand J Immunol. 1975;4(4):309–320. doi: 10.1111/j.1365-3083.1975.tb02631.x. [DOI] [PubMed] [Google Scholar]
  9. Craig S. W., Cebra J. J. Peyer's patches: an enriched source of precursors for IgA-producing immunocytes in the rabbit. J Exp Med. 1971 Jul 1;134(1):188–200. doi: 10.1084/jem.134.1.188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Craig S. W., Cebra J. J. Rabbit Peyer's patches, appendix, and popliteal lymph node B lymphocytes: a comparative analysis of their membrane immunoglobulin components and plasma cell precursor potential. J Immunol. 1975 Jan;114(1 Pt 2):492–502. [PubMed] [Google Scholar]
  11. Eskeland T., Brandtzaeg P. Does J chain mediate the combination of 19S IgM and dimeric IgA with the secretory component rather than being necessary for their polymerization? Immunochemistry. 1974 Mar;11(3):161–163. doi: 10.1016/0019-2791(74)90214-6. [DOI] [PubMed] [Google Scholar]
  12. Eskeland T. IgM reassociation in Vitro: no influence of J chain on the amount of polymers. Scand J Immunol. 1974;3(6):757–768. doi: 10.1111/j.1365-3083.1974.tb01311.x. [DOI] [PubMed] [Google Scholar]
  13. Eskeland T., Klein E., Inoue M., Johansson B. Characterization of immunoglobulin structures from the surface of chronic lymphocytic leukemia cells. J Exp Med. 1971 Jul 1;134(1):265–280. doi: 10.1084/jem.134.1.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Fu S. M., Kunkel H. G. Membrane immunoglobulins of B lymphocytes: inability to detect certain characteristic IgM and IgD antigens. J Exp Med. 1974 Oct 1;140(4):895–903. doi: 10.1084/jem.140.4.895. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Guy-Grand D., Griscelli C., Vassalli P. Gut-associated lymphoblasts and intestinal IgA plasma cells. Adv Exp Med Biol. 1974;45(0):41–46. doi: 10.1007/978-1-4613-4550-3_5. [DOI] [PubMed] [Google Scholar]
  16. Jubert A. V., Mc Bride C. M., Mavligit G., Gutterman J. U., Hersh E. M. Immunoglobulins on the surface of human appendix lymphocytes. Immunol Commun. 1974;3(1):1–9. doi: 10.3109/08820137409055741. [DOI] [PubMed] [Google Scholar]
  17. Knapp W., Bolhuis R. L., Rádl J., Hijmans W. Independent movement of IgD and IgM molecules on the surface of individual lymphocytes. J Immunol. 1973 Oct;111(4):1295–1298. [PubMed] [Google Scholar]
  18. Koshland M. E. Structure and function of the J chain. Adv Immunol. 1975;20:41–69. doi: 10.1016/s0065-2776(08)60206-0. [DOI] [PubMed] [Google Scholar]
  19. McWilliams M., Phillips-Quagliata J. M., Lamm M. E. Characteristics of mesenteric lymph node cells homing to gut-associated lymphoid tissue in syngeneic mice. J Immunol. 1975 Jul;115(1):54–58. [PubMed] [Google Scholar]
  20. Merler E., Gatien J., DeWilde G. Significance of immunofluorescent staining of lymphocytes with antisera to IgM immunoglobulins. Nature. 1974 Oct 18;251(5476):652–654. doi: 10.1038/251652a0. [DOI] [PubMed] [Google Scholar]
  21. Pernis B., Brouet J. C., Seligmann M. IgD and IgM on the membrane of lymphoid cells in macroglobulinemia. Evidence for identity of membrane IgD and IgM antibody activity in a case with anti-IgG receptors. Eur J Immunol. 1974 Nov;4(11):776–778. doi: 10.1002/eji.1830041114. [DOI] [PubMed] [Google Scholar]
  22. Ploem J. S. The use of a vertical illuminator with interchangeable dichroic mirrors for fluorescence microscopy with incidental light. Z Wiss Mikrosk. 1967 Nov;68(3):129–142. [PubMed] [Google Scholar]
  23. Preud'Homme J. L., Clauvel J. P., Seligmann M. Immunoglobulin D-bearing lymphocytes in primary immunodeficiencies. J Immunol. 1975 Jan;114(1 Pt 2):481–485. [PubMed] [Google Scholar]
  24. Rowe D. S., Hug K., Forni L., Pernis B. Immunoglobulin D as a lymphocyte receptor. J Exp Med. 1973 Oct 1;138(4):965–972. doi: 10.1084/jem.138.4.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rudzik O., Clancy R. L., Perey D. Y., Bienenstock J., Singal D. P. The distribution of a rabbit thymic antigen and membrane immunoglobulins in lymphoid tissue, with special reference to mucosal lymphocytes. J Immunol. 1975 Jan;114(1 Pt 1):1–4. [PubMed] [Google Scholar]
  26. Salsano F., Froland S. S., Natvig J. B., Michaelsen T. E. Same idiotype of B-lymphocyte membrane IgD and IgM. Formal evidence for monoclonality of chronic lymphocytic leukemia cells. Scand J Immunol. 1974;3(6):841–846. doi: 10.1111/j.1365-3083.1974.tb01321.x. [DOI] [PubMed] [Google Scholar]
  27. Uhr J. W., Vitetta E. S. Cell surface immunoglobulin. 8. Synthesis, secretion, and cell surface expression of immunoglobulin in murine thoracic duct lymphocytes. J Exp Med. 1974 Apr 1;139(4):1013–1018. doi: 10.1084/jem.139.4.1013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Vitetta E. S., Baur S., Uhr J. W. Cell surface immunoglobulin. II. Isolation and characterization of immunoglobulin from mouse splenic lymphocytes. J Exp Med. 1971 Jul 1;134(1):242–264. doi: 10.1084/jem.134.1.242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Williams A. F., Gowans J. L. The presence of IgA on the surface of rat thoractic duct lymphocytes which contain internal IgA. J Exp Med. 1975 Feb 1;141(2):335–345. doi: 10.1084/jem.141.2.335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Winchester R. J., Fu S. M., Hoffman T., Kunkel H. G. IgG on lymphocyte surfaces; technical problems and the significance of a third cell population. J Immunol. 1975 Apr;114(4):1210–1212. [PubMed] [Google Scholar]

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