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. 1976 Jul;25(1):144–151.

An extractable nuclear antigen not attaching to tannic acid-treated erythrocytes.

J Jonsson, M Steiner, E Klein
PMCID: PMC1541385  PMID: 791543

Abstract

One of the antigens present in isotonic phosphate buffer extract of calf thymus nuclei against which antibodies are produced in collagen diseases, was shown not to attach to tanned sheep erythrocytes. It is, however, well exposed on slides coated with smears of ENA-extract and fixed with methanol-acetone. Reactions with the antigen can be demonstrated by the mixed haemadsorption technique. Preliminary results suggest that it may be a ribonucloprotein closely related to the 'soluble ribonucleoprotein" (sRNP) attachable to tanned erythrocytes. It may also have a similar relationship to clinical symptoms.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOYDEN S. V. The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera. J Exp Med. 1951 Feb;93(2):107–120. doi: 10.1084/jem.93.2.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. COOMBS R. R., BEDFORD D., ROUILLARD L. M. A and B blood-group antigens on human epidermal cells demonstrated by mized agglutination. Lancet. 1956 Apr 21;270(6921):461–463. doi: 10.1016/s0140-6736(56)90528-1. [DOI] [PubMed] [Google Scholar]
  3. Fagraeus A., Espmark J. A., Jonsson J. Mixed haemadsorption: a mixed antiglobulin reaction applied to antigens on a glass surface. Preparation and evaluation of indicator red cells; survey of present applications. Immunology. 1965 Aug;9(2):161–175. [PMC free article] [PubMed] [Google Scholar]
  4. JOHNSON J. APPLICATION OF MIXED HAEMADSORPTION--A MIXED ANTIGLOBULIN REACTION--TO SOLUBLE ANTIGENS FIXED ON GLASS SLIDES. I. DESCRIPTION OF TECHNICAL PROCEDURE AND SOME EXAMPLES OF ITS APPLICATION. Int Arch Allergy Appl Immunol. 1965;27:157–174. [PubMed] [Google Scholar]
  5. Jonsson J. Application of mixed haemadsorption--a mixed antiglobulin reaction--to soluble antigens fixed on glass slides. Acta Pathol Microbiol Scand. 1969;(Suppl):1+–1+. doi: 10.1159/000229609. [DOI] [PubMed] [Google Scholar]
  6. Jonsson J. The incidence of antibodies to a soluble nuclear antigen in the sera of patients with systemic lupus erythematosus and rheumatoid arthritis estimated with the mixed haemadsorption technique. Clin Exp Immunol. 1967 Jul;2(4):415–426. [PMC free article] [PubMed] [Google Scholar]
  7. Reedman B. M., Klein G. Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines. Int J Cancer. 1973 May;11(3):499–520. doi: 10.1002/ijc.2910110302. [DOI] [PubMed] [Google Scholar]
  8. SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
  9. Seiner M., Klein E., Klein G. Antinuclear reactivity of sera in patients with leukemia and other neoplastic diseases. Clin Immunol Immunopathol. 1975 Sep;4(3):374–381. doi: 10.1016/0090-1229(75)90006-9. [DOI] [PubMed] [Google Scholar]
  10. Sharp G. C., Irvin W. S., LaRoque R. L., Velez C., Daly V., Kaiser A. D., Holman H. R. Association of autoantibodies to different nuclear antigens with clinical patterns of rheumatic disease and responsiveness to therapy. J Clin Invest. 1971 Feb;50(2):350–359. doi: 10.1172/JCI106502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sharp G. C., Irvin W. S., Tan E. M., Gould R. G., Holman H. R. Mixed connective tissue disease--an apparently distinct rheumatic disease syndrome associated with a specific antibody to an extractable nuclear antigen (ENA). Am J Med. 1972 Feb;52(2):148–159. doi: 10.1016/0002-9343(72)90064-2. [DOI] [PubMed] [Google Scholar]
  12. Tan E. M. Relationship of nuclear staining patterns with precipitating antibodies in systemic lupus erythematosus. J Lab Clin Med. 1967 Nov;70(5):800–812. [PubMed] [Google Scholar]
  13. WELLER T. H., COONS A. H. Fluorescent antibody studies with agents of varicella and herpes zoster propagated in vitro. Proc Soc Exp Biol Med. 1954 Aug-Sep;86(4):789–794. doi: 10.3181/00379727-86-21235. [DOI] [PubMed] [Google Scholar]
  14. Yasuda-Yasaki Y., Yoshida T. O. Isolation of "speckled" nuclear antigen reactive with autoantibodies in patients with cancer and autoimmune diseases. Scand J Immunol. 1975;4(4):357–367. doi: 10.1111/j.1365-3083.1975.tb02637.x. [DOI] [PubMed] [Google Scholar]

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