Skip to main content
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1976 Jul;25(1):122–132.

Heterophile antibodies in pathologic human sera resembling antibodies stimulated by foreign species sera.

R Kasukawa, K Kano, M L Bloom, F Milgrom
PMCID: PMC1541393  PMID: 825336

Abstract

Studies were performed on heterphile antibodies originally described by Hanganutziu and Deicher and referred to as H-D antibodies. It was confirmed that these antibodies appear as a result of injections of foreign species sera. They differ from Forssman antibodies by combining with bovine erythrocytes and from Paul-Bunnell antibodies by reacting with guinea-pig kidney. it was demonstrated that H-D antibodies react in double diffusion gel precipitation tests with: (1) crude extract of bovine erythrocyte stromata; (2) purified fraction of this extract devoid of Paul-Bunnell antigen; (3) whole bovine serum and sera of several other species; and (4) thermostable ethanol-insoluble freactions of serum and organs of oxen and several other species. These various antigenic preparations gave usually reactions of complete or partial identity with each other. In several instances, two or even three precipitation lines could be detected. H-D negative human erythrocytes became coated with H-D antigen upon simple incubation with H-D-positive sera. H-D antibodies were also detected in some pathological human sera without any indication that the patients had ever received injections of foreign species sera. Such antibodies were undistinguishable from H-D antibodies engendered by injections of foreign sera.

Full text

PDF
122

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Juji T., Kano K., Milgrom F. Antibody-induced lysis of nucleated cells in agar gel. Int Arch Allergy Appl Immunol. 1971;41(5):739–753. doi: 10.1159/000230565. [DOI] [PubMed] [Google Scholar]
  2. KANO K., MILGROM F. REACTIONS OF SERUM SICKNESS AND INFECTIOUS MONONUCLEOSIS SERA IN TISSUE CULTURES. Proc Soc Exp Biol Med. 1964 Jan;115:172–173. doi: 10.3181/00379727-115-28861. [DOI] [PubMed] [Google Scholar]
  3. MILGROM F., TUGGAC Z. M., WITEBSKY E. ORGAN-SPECIFIC ANTIGENS OF LIVER, TESTICLE AND PITUITARY. J Immunol. 1965 Jan;94:157–163. [PubMed] [Google Scholar]
  4. MILGROM F., WITEBSKY E. Immunological studies on adrenal glands. I. Immunization with adrenals of foreign species. Immunology. 1962 Jan;5:46–66. [PMC free article] [PubMed] [Google Scholar]
  5. Malavé I., Kano K., Milgrom F. Reactions fo murine lymphoma cells with infectious mononucleosis sera. J Immunol. 1973 Feb;110(2):439–443. [PubMed] [Google Scholar]
  6. Malavé I., Milgrom F. Heterophile antigen detected by infectious mononucleosis sera on lymphoid cells. Int Arch Allergy Appl Immunol. 1973;44(5):601–610. doi: 10.1159/000230964. [DOI] [PubMed] [Google Scholar]
  7. Milgrom F., Loza U. Agglutination of particulate antigens in agar gel. J Immunol. 1967 Jan;98(1):102–109. [PubMed] [Google Scholar]
  8. Milgrom F., Loza U. Complement-mediated hemolysis in agar gel. J Immunol. 1966 Mar;96(3):415–423. [PubMed] [Google Scholar]
  9. Milgrom F., Loza U., Kano K. Double diffusion in gel tests with Paul-Bunnell antibodies of infectious mononucleosis sera. Int Arch Allergy Appl Immunol. 1975;48(1):82–93. doi: 10.1159/000231294. [DOI] [PubMed] [Google Scholar]
  10. Pirofsky B., Ramirez-Mateos J. C., August A. "Foreign serum" heterophile antibodies in patients receiving antithymocyte antisera. Blood. 1973 Sep;42(3):385–393. [PubMed] [Google Scholar]
  11. Rendel J, Sorensen A N, Irwin M R. Evidence for Epistatic Action of Genes for Antigenic Substances in Sheep. Genetics. 1954 May;39(3):396–408. doi: 10.1093/genetics/39.3.396. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SCHEIDEGGER J. J. Une micro-méthode de l'immuno-electrophorèse. Int Arch Allergy Appl Immunol. 1955;7(2):103–110. [PubMed] [Google Scholar]
  13. WITEBSKY E., ROSE N. R. Studies on organ specificity. IV. Production of rabbit thyroid antibodies in the rabbit. J Immunol. 1956 Jun;76(6):408–416. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES