Skip to main content
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1976 Jul;25(1):180–183.

A simple immunoenzymatic method for measuring IgE in human sera.

J L Gluesdon, R Thierry, S Avrameas
PMCID: PMC1541397  PMID: 791545

Abstract

A reliable and relatively simple method for the estimation of serum IgE by single radial immunodiffusion is described. The method requires glucose oxidase-labelled antibodies. The method permits the measurement of IgE concentrations ranging from 20 to 700 i.u./ml. The values obtained in unknown samples were in good agreement with those obtained by radioimmunoassay (correlation coefficient r=0-9557).

Full text

PDF
180

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arbesman C. E., Ito K., Wypych J. I., Wicher K. Measurement of serum IgE by a one-step single radial radiodiffusion method. J Allergy Clin Immunol. 1972 Feb;49(2):72–80. doi: 10.1016/0091-6749(72)90058-9. [DOI] [PubMed] [Google Scholar]
  2. Avrameas S. Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies. Immunochemistry. 1969 Jan;6(1):43–52. doi: 10.1016/0019-2791(69)90177-3. [DOI] [PubMed] [Google Scholar]
  3. Avrameas S., Ternynck T. The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immunochemistry. 1969 Jan;6(1):53–66. doi: 10.1016/0019-2791(69)90178-5. [DOI] [PubMed] [Google Scholar]
  4. Centifanto Y. M., Kaufman H. E. A simplified method for measuring human IgE. J Immunol. 1971 Aug;107(2):608–609. [PubMed] [Google Scholar]
  5. FAHEY J. L., MCKELVEY E. M. QUANTITATIVE DETERMINATION OF SERUM IMMUNOGLOBULINS IN ANTIBODY-AGAR PLATES. J Immunol. 1965 Jan;94:84–90. [PubMed] [Google Scholar]
  6. Guesdon J. L., Avrameas S. An immunoenzymatic method for measuring low concentrations of antigens by single radial diffusion. Immunochemistry. 1974 Sep;11(9):595–598. doi: 10.1016/0019-2791(74)90251-1. [DOI] [PubMed] [Google Scholar]
  7. Jalanti R., Henney C. S. Studies on single radial immunodiffusion techniques for the quantitation of antigen and antibody. J Immunol Methods. 1972 Jan;1(2):123–131. doi: 10.1016/0022-1759(72)90039-7. [DOI] [PubMed] [Google Scholar]
  8. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  9. Nezlin R. S., Zagyansky Y. A., Käiväräinen A. I., Stefani D. V. Properties of myeloma immunoglobulin E(Yu). Chemical, fluorescence polarisation and spin-labeled studies. Immunochemistry. 1973 Oct;10(10):681–688. doi: 10.1016/0019-2791(73)90211-5. [DOI] [PubMed] [Google Scholar]
  10. Rowe D. S. Radioactive single radial diffusion: a method for increasing the sensitivity of immunochemical quantification of proteins in agar gel. Bull World Health Organ. 1969 Apr;40(4):613–616. [PMC free article] [PubMed] [Google Scholar]
  11. Ternynck T., Avrameas S. Polyacrylamide-protein immunoadsorbents prepared with glutaraldehyde. FEBS Lett. 1972 Jun 1;23(1):24–28. doi: 10.1016/0014-5793(72)80274-6. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES